The chemistry and biochemistry of simple and complex lipids

Christie, William W.

doi:10.1039/np9840100499

Cited by 10 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipid degradation can occur by hydrolysis (release of FFA from acyl lipids through the enzymatic activity of lipases) or oxidation of carbon-carbon double bonds. In view of the labile nature of lipids, these processes often occur very rapidly, leading to analytical errors and increased sample variability (Christie, 1984). Handling and storage conditions and methods can help minimize sample degradation and analytical bias.…”

Section: Sample Handling and Storage Before Lipid Extractionmentioning

confidence: 99%

“…The rate of oxidation and hydrolysis processes (hereafter referred to as "lipid degradation") is likely to vary greatly across species and tissue types. These processes can be revealed by an increase in FFA and a decrease in glycerolipids and PUFA in samples (Christie, 1984, see section Indicators of sample degradation). Here, we provide an overview of different methods used in the literature and discuss their strengths and limitations.…”

Section: Sample Handling and Storage Before Lipid Extractionmentioning

confidence: 99%

“…It can lead to increased FFA and products of oxidation (e.g. Christie, 1984;Gullian-Klanian et al, 2017;Tenyang et al, 2019). Lower freezing temperatures and short-term storage are generally associated with lower lipid degradation (e.g.…”

Section: Storagementioning

confidence: 99%

“…One or several FA are linked either with an ester (or ether/vinyl ether) bond to an alcohol, mainly glycerol, or with an amide linkage to a long-chain nitrogen base, mainly sphingosine. All of these molecules are categorized as either neutral lipids or polar lipids, with reference to chromatographic methods of lipid class separation but also to their metabolic roles (Christie, 1984). Among neutral lipids, FA-containing compounds include TAG and WE, which are the main storage lipids, whereas DAG, free FA (FFA), and MAG are generally minor constituents.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

State of art and best practices for fatty acid analysis in aquatic sciences

Couturier

Michel

Amaro

et al. 2020

ICES Journal of Marine Science

View full text Add to dashboard Cite

Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.

show abstract

Section: Sample Handling and Storage Before Lipid Extractionmentioning

confidence: 99%

Section: Sample Handling and Storage Before Lipid Extractionmentioning

confidence: 99%

Section: Storagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

State of art and best practices for fatty acid analysis in aquatic sciences

Couturier

Michel

Amaro

et al. 2020

ICES Journal of Marine Science

View full text Add to dashboard Cite

show abstract

“…Furthermore, lipids can be regarded as simple (sterols, fatty acids, etc.) or complex (glycerophospholipids, glycolipids); the former yields, at most, two products after hydrolysis, and the latter results in three or more products [2,3].…”

Section: Introductionmentioning

confidence: 99%

A Gas Chromatography-Mass Spectrometry Method for the Determination of Fatty Acids and Sterols in Yeast and Grape Juice

et al. 2021

View full text Add to dashboard Cite

Lipids are essential components of all living cells. In an oenological context, the supply of unsaturated lipids in grape juice allows the yeasts to grow and ferment, despite very low levels of oxygen. The current study proposes a systematic optimization procedure for the analysis of fatty acids and sterols relevant to the grape fermentation process, including both extracellular and intracellular (i.e., yeast cells) lipids. Even though it was extensive, the sample preparation yielded reproducible results for all compounds of interest. The stability of the analyzed compounds was also tested to offer some implementation flexibility for the extensive procedure. The performance parameters (i.e., selectivity, linearity, limit of detection and quantitation, accuracy, and precision) indicated that the method was suitable for future practical implementation. The proof of concept also suggests that the list of compounds of interest can be expanded if additional peaks are identified. Given the large variation in concentrations, the dilution of the matrix needs to be carefully considered in order to ensure that the lipids of interest are still within the dynamic range and not below the limit of detection and/or quantification.

show abstract

ChemInform Abstract: THE CHEMISTRY AND BIOCHEMISTRY OF SIMPLE AND COMPLEX LIPIDS

Christie¹

1985

Chemischer Informationsdienst

View full text Add to dashboard Cite

The chemistry and biochemistry of simple and complex lipids

Cited by 10 publications

References 36 publications

State of art and best practices for fatty acid analysis in aquatic sciences

State of art and best practices for fatty acid analysis in aquatic sciences

A Gas Chromatography-Mass Spectrometry Method for the Determination of Fatty Acids and Sterols in Yeast and Grape Juice

ChemInform Abstract: THE CHEMISTRY AND BIOCHEMISTRY OF SIMPLE AND COMPLEX LIPIDS

Contact Info

Product

Resources

About