The chemistry of lysozyme and its use as a food preservative and a pharmaceutical

Proctor, V. A.; Cunningham, F. E.

doi:10.1080/10408398809527473

Cited by 367 publications

(251 citation statements)

References 123 publications

Supporting

Mentioning

244

Contrasting

Unclassified

Order By: Relevance

“…27.6 g/ml) and cabbage (ca. 2.3 g/ml) (22). The use of these naturally occurring lysozymes at a concentration of 10 to 100 g/ml, as proposed in this work, should therefore not present a toxicological concern.…”

mentioning

confidence: 98%

Inactivation of Gram-Negative Bacteria by Lysozyme, Denatured Lysozyme, and Lysozyme-Derived Peptides under High Hydrostatic Pressure

Masschalck

Houdt

Haver

et al. 2001

Appl Environ Microbiol

140

View full text Add to dashboard Cite

We have studied the inactivation of six gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens, Salmonella enterica serovar Typhimurium, Salmonella enteritidis, Shigella sonnei, and Shigella flexneri) by high hydrostatic pressure treatment in the presence of hen egg-white lysozyme, partially or completely denatured lysozyme, or a synthetic cationic peptide derived from either hen egg white or coliphage T4 lysozyme. None of these compounds had a bactericidal or bacteriostatic effect on any of the tested bacteria at atmospheric pressure. Under high pressure, all bacteria except both Salmonella species showed higher inactivation in the presence of 100 g of lysozyme/ml than without this additive, indicating that pressure sensitized the bacteria to lysozyme. This extra inactivation by lysozyme was accompanied by the formation of spheroplasts. Complete knockout of the muramidase enzymatic activity of lysozyme by heat treatment fully eliminated its bactericidal effect under pressure, but partially denatured lysozyme was still active against some bacteria. Contrary to some recent reports, these results indicate that enzymatic activity is indispensable for the antimicrobial activity of lysozyme. However, partial heat denaturation extended the activity spectrum of lysozyme under pressure to serovar Typhimurium, suggesting enhanced uptake of partially denatured lysozyme through the serovar Typhimurium outer membrane. All test bacteria were sensitized by high pressure to a peptide corresponding to amino acid residues 96 to 116 of hen egg white, and all except E. coli and P. fluorescens were sensitized by high pressure to a peptide corresponding to amino acid residues 143 to 155 of T4 lysozyme. Since they are not enzymatically active, these peptides probably have a different mechanism of action than all lysozyme polypeptides.High hydrostatic pressure treatment is a promising technique for cold pasteurization of foods that allows better retention of product flavor, texture, color, and nutrient content than a comparable conventional heat pasteurization (17, 24). The main obstacles that prevent a commercial breakthrough of pressure-preserved foods are the high investment cost, due to the high pressures required for efficient microbe and enzyme destruction, and the paucity of knowledge on the sensitivity of various pathogenic and spoilage microorganisms to hydrostatic pressure and on the factors affecting this sensitivity.The application of hurdle technology has been proposed as an approach to increase the microbicidal effect of the process at lower pressures. Hurdle technology relies on the synergistic combination of moderate doses of two or more microbe-inactivating and/or growth-retarding factors (18). An interesting example of synergistic inactivation exists between high pressure and a number of antimicrobial peptides, including nisin, lysozyme, and pediocin (3,9,14,19). This synergistic inactivation was observed not only in intrinsically sensitive gram-positive bacteria but also in gram-negative bacteria, which a...

show abstract

mentioning

confidence: 98%

Inactivation of Gram-Negative Bacteria by Lysozyme, Denatured Lysozyme, and Lysozyme-Derived Peptides under High Hydrostatic Pressure

Masschalck

Houdt

Haver

et al. 2001

Appl Environ Microbiol

140

View full text Add to dashboard Cite

show abstract

“…Residual levels of 0.06-327 mg/L were found in lysozyme-treated wines [22,23], while in commercial cheeses, concentrations of 30.8-386.2 mg/kg were found to be present [24]. Lysozyme has also been used as an antibacterial agent during beer production [25], to extend the shelf-life of meat [26] and shrimp [27], as an alternative to antibiotics [28,29] in veterinary medicine or as anti-inflammatory drug in the treatment of wounds and infections [4,30].…”

Section: Properties Of Lysozyme and Its Importance For Daily Lifementioning

confidence: 99%

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

View full text Add to dashboard Cite

Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

show abstract

“…7,8 Unlike many antimicrobials, LSZ has both enzymatic and nonenzymatic activity in both its native and denatured states and is useful even in processes which require heat treatment. The potential use of LSZ as an antimicrobial agent in pharmaceuticals, food preservatives, and packaging is an active area of research, 7,8 but the effective use of LSZ requires incorporating it with a more mechanically robust material. SWNTs are well-known for exceptional combination of mechanical, electrical, thermal, and optical properties.…”

mentioning

confidence: 99%

Strong Antimicrobial Coatings: Single-Walled Carbon Nanotubes Armored with Biopolymers

et al. 2008

View full text Add to dashboard Cite

Large scale biomimetic single-walled carbon nanotube (SWNT) coatings with significant antimicrobial activity, high Young's Modulus, and controlled morphology were fabricated using layer-by-layer assembly. Thickness was controlled within 1.6 nm and SWNT orientation was controlled using a directed air stream. This unique blend of multifunctionality and vertical and lateral control of a bottom-up assembly process is a significant advancement in developing macroscale assemblies with the combined attributes of SWNTs and natural materials.Concern about the spread of infections through contact with contaminated surfaces was once limited to specific groups of people including astronauts who are subject to confined living spaces and the virulence-enhancing effects of space flight 1 and people requiring surgery or implantable devices. 2 More recently, there has been growing concern about the role of contaminated surfaces in the spread of infections such as severe acute respiratory syndrome (SARS), 3,4 and Staphylococcus aureus, particularly methicillin-resistant Staphylococcus aureus (MSRA). 5 Antimicrobial surfaces are therefore desirable not only for the aerospace, defense, and medical industries but also for the consumer product and public transportation industries. We have used layer-by-layer assembly to produce coatings that combine the strength of single-walled carbon nanotubes (SWNTs) with the antimicrobial activity of lysozyme (LSZ).LSZ, a key member of ova-antimicrobials, is a powerful natural antibacterial protein. 6 It is in the class of enzymes which lyse the cell walls of Gram-positive bacteria by hydrolyzing the -1,4 linkage between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) of gigantic polymers in the peptidoglycan (murein). 7,8 Unlike many antimicrobials, LSZ has both enzymatic and nonenzymatic activity in both its native and denatured states and is useful even in processes which require heat treatment. The potential use of LSZ as an antimicrobial agent in pharmaceuticals, food preservatives, and packaging is an active area of research, 7,8 but the effective use of LSZ requires incorporating it with a more mechanically robust material. SWNTs are well-known for exceptional combination of mechanical, electrical, thermal, and optical properties. 9-11 However, the efficient transfer of SWNTs' inherent nanoscale properties to macroscopic structures and devices has been an ongoing research challenge comprised of three main issues: SWNT dispersion, controlled assembly, and efficient load transfer. There has been growing interest in using biological materials to stabilize dispersions of pristine SWNTs. DNA enables much higher concentrations of dispersions of individual and small bundles of SWNTs 11,12 than any other known material besides superacids; 13,14 DNA-SWNT dispersions have even been used to produce liquid crystalline dispersions for solution spinning. 15 Similarly, favorable intermolecular interactions enable dispersion of individual and small bundles of SWNTs in proteins such as LSZ...

show abstract

The chemistry of lysozyme and its use as a food preservative and a pharmaceutical

Cited by 367 publications

References 123 publications

Inactivation of Gram-Negative Bacteria by Lysozyme, Denatured Lysozyme, and Lysozyme-Derived Peptides under High Hydrostatic Pressure

Inactivation of Gram-Negative Bacteria by Lysozyme, Denatured Lysozyme, and Lysozyme-Derived Peptides under High Hydrostatic Pressure

Aptamer-Based Electrochemical Sensing of Lysozyme

Strong Antimicrobial Coatings: Single-Walled Carbon Nanotubes Armored with Biopolymers

Contact Info

Product

Resources

About