The chemopreventive effect of diallyl disulphide on N-nitrosodiethylamine induced heptocarcinogenesis

Manivasagam, Thamilarasan; Subramanian, Perumal; Ganapathy, Suthakar; Essa, Musthafa Mohamed

doi:10.32725/jab.2005.024

Cited by 6 publications

(1 citation statement)

References 25 publications

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“…Diallyl disulfide (DADS) is an oil-soluble organosulfur compound obtained from garlic and is known to be effective against carcinogenesis and chemically induced toxicity in humans (Manivasagam et al, 2005; Song et al, 2009). In addition to this, it has also been shown to inhibit CYP2E1 metabolism (Ehnert et al, 2012; Shimada et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Protective Role of CYP2E1 Inhibitor Diallyl Disulfide (DADS) on Alcohol-Induced Malondialdehyde-Deoxyguanosine (M1dG) Adduct Formation

Sapkota¹,

Hottor²,

DeVasure

et al. 2014

Alcohol Clin Exp Res

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Background Alcohol use disorders are often associated with lung disease. Alcohol exposure leads to the production of reactive oxygen species, lipid peroxidation, and formation of malondialdehyde (MDA) as well as induce the expression of cytochrome p450 2E1 (CYP2E1). Likewise, cigarette smoking can lead to lung lipid peroxidation and formation of MDA. MDA can bind to DNA forming MDA deoxyguanosine (M1dG) adducts, which have been implicated in alcohol-related cancers and cardiovascular disease. Because CYP2E1 regulates MDA production, and our previous studies have shown that alcohol and cigarette smoke can lead to MDA formation, we hypothesized that CYP2E1 would modulate M1dG adduct formation and single strand DNA damage in alcohol- and cigarette smoke-exposed lung cells and tissue. Methods Normal human bronchial epithelial cells (HBEC) were pre-treated with 10 μM DADS for 1h, and treated with 80 mM ethanol +/− 5% cigarette smoke extract (CSE) for 3 hrs for comet assay and 6 hrs for CYP2E1, MDA, and M1dG adduct assays. C57BL/6 mice were administered 20% ethanol ad libitum in drinking water for 8 wk and exposed to whole body cigarette smoke for 5 wk. Mice were also fed a CYP2E1 inhibitor, diallyl disulfide (DADS), at 1 μM/g of feed in their daily diet for 7 wk. Whole lung tissue homogenate was used for CYP2E1, MDA, and M1dG adduct assays. Results Ethanol exposure significantly increased HBEC olive tail moment. DADS pretreatment of HBEC attenuated this ethanol effect. Ethanol also induced MDA and M1dG adduct formation, which was also significantly reduced by DADS treatment. CSE +/− ethanol did not enhance these effects. In lung tissue homogenate of 8 wk alcohol-fed mice, MDA and M1dG adduct levels were significantly elevated in comparison to control mice and mice fed DADS while consuming alcohol. No increase in MDA and M1dG adduct formation was observed in 5 wk cigarette smoke-exposed mice. Conclusions These findings suggest that CYP2E1 plays a pivotal role in alcohol-induced M1dG adducts, and the use of DADS as dietary supplement can reverse the effects of alcohol on M1dG formation.

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Section: Introductionmentioning

confidence: 99%

Protective Role of CYP2E1 Inhibitor Diallyl Disulfide (DADS) on Alcohol-Induced Malondialdehyde-Deoxyguanosine (M1dG) Adduct Formation

Sapkota¹,

Hottor²,

DeVasure

et al. 2014

Alcohol Clin Exp Res

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Role of biological clocks in cancer processes and chronotherapy

Subramanian

Kumaravel

Manivasagam

2010

Biological Rhythm Research

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The genus Mansoa (Bignoniaceae): a source of organosulfur compounds

Zoghbi¹,

Oliveira²,

Guilhon³

2009

Rev. bras. farmacogn.

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RESUMO: "O gênero Mansoa (Bignoniaceae): uma fonte de compostos organosulfurados." O gênero Mansoa pertence à família Bignoniaceae e inclui onze espécies que ocorrem principalmente nas florestas secas e úmidas do Brasil e da Argentina até o Sudeste do México. Essas espécies na Amazônia brasileira são conhecidas como "cipó-de-alho", em referência ao forte cheiro de alho das folhas quando esmagadas. O "cipó-de-alho" tem vários usos na medicina tradicional e entre eles, os mais citados são para tratamento de gripe, febre, dor e inflamação de artrite e reumatismo. Apesar de todos os usos, ainda tem pequena aplicação como fitoterápico quando comparado ao alho (Allium sativum). Os óleos essenciais de Mansoa spp. contêm polissulfetos de alila que contribuem para o aroma e sabor característicos. A composição química dos extratos orgânicos de Mansoa incluiu alcanos, alcanóis, triterpenóides, flavonóides, derivados do lapachol e o derivado sulfurado aliína. Os usos, composição química, atividades biológicas e aspectos agrícolas de espécies de Mansoa e sua relação com A. sativum são apresentados.Unitermos: Mansoa, Bignoniaceae, composição química, atividades biológicas, aspectos agrícolas. ABSTRACT:The genus Mansoa belongs to the family Bignoniaceae and it includes eleven species that occur mainly in the dry and wet forests of Brazil and from Argentina to the Southeast of Mexico. These species in the Brazilian Amazon region are known as "cipó-de-alho", that means garlic vine, in reference to the pungent garlic-like smell of the leaves when crushed. "Cipó-dealho" has several uses in folk medicine and among them, the most cited are the treatment for cold, fever, pain and inflammation of arthritis and rheumatism. In spite of all those uses, it still has little application in phytotherapy when compared to garlic (Allium sativum). The essential oils of Mansoa spp. show the presence of allyl polysufides that contribute to the characteristic aroma and flavor. The chemical composition of the organic extracts of Mansoa has been reported and it includes alkanes, alkanols, triterpenoids, flavonoids, lapachol derivatives and organosulfur compound alliin. The uses, chemical composition, biological activities and agricultural aspects of Mansoa species and their relationship with A. sativum are presented.

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The chemopreventive effect of diallyl disulphide on N-nitrosodiethylamine induced heptocarcinogenesis

Cited by 6 publications

References 25 publications

Protective Role of CYP2E1 Inhibitor Diallyl Disulfide (DADS) on Alcohol-Induced Malondialdehyde-Deoxyguanosine (M1dG) Adduct Formation

Protective Role of CYP2E1 Inhibitor Diallyl Disulfide (DADS) on Alcohol-Induced Malondialdehyde-Deoxyguanosine (M1dG) Adduct Formation

Role of biological clocks in cancer processes and chronotherapy

The genus Mansoa (Bignoniaceae): a source of organosulfur compounds

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