The chemopreventive properties of chlorogenic acid reveal a potential new role for the microsomal glucose-6-phosphate translocase in brain tumor progression

Belkaid, Anissa; Currie, Jean-Christophe; Desgagnés, Julie; Annabi, Borhane

doi:10.1186/1475-2867-6-7

Cited by 131 publications

(63 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gelatin Zymography-Gelatin zymography was used to assess the extent of pro-MMP-2 activation in the culture medium as described previously (19). Gelatinolytic activity was detected as unstained bands on a blue background.…”

Section: Methodsmentioning

confidence: 99%

“…One microgram of total RNA was used for first strand cDNA synthesis followed by specific gene product amplification with the One-Step RT-PCR kit (Invitrogen). Primer sequences for MT1-MMP, G6PT, glucose-6-phosphatase (G6Pase)-␤, and glyceraldehyde-3-phosphate dehydrogenase have been validated and published elsewhere (19). PCR conditions were optimized so that the gene products were examined at the exponential phase of their amplification, and the products were resolved on 1.5% agarose gels containing 1 g/ml ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

“…Given that impaired chemotaxis was recently observed in bone marrow cells isolated from a microsomal glucose 6-phos-phate transporter-deficient (G6PT Ϫ/Ϫ ) mouse model (17,18) and that G6PT was demonstrated to regulate cell migration (19), we hypothesized whether MT1-MMP and G6PT might share some common molecular and cellular impact on the regulation of BMSC chemotaxis and ECM degradation. Since its discovery, G6PT has been shown to integrate and regulate many metabolic functions such as glycemia, lipidmia, uricemia, and lactic acidemia (20).…”

mentioning

confidence: 99%

“…The originality of our research relies upon the combined evidence that cell surface MMP signaling is essential for BMSC chemotaxis (14) and that G6PT may function as an intracellular "bio-switch" in cell death versus cell mobilization in BMSC (19). We decided to investigate the potential molecular and functional link that may exist between MT1-MMP and G6PT functions.…”

mentioning

confidence: 99%

“…More importantly, its activity cannot be substituted as G6PT deficiencies lead to glycogen storage disease type Ib characterized, not only by disturbed glucose homeostatsis but by severe myeloid dysfunctions (20). Lack of G6PT function alters, for instance, neutrophil chemotaxis and calcium flux (21), and its expression inhibition decreases cell survival (19,22,23).…”

mentioning

confidence: 99%

See 4 more Smart Citations

MT1-MMP Down-regulates the Glucose 6-Phosphate Transporter Expression in Marrow Stromal Cells

Currie¹,

Fortier²,

Sina³

et al. 2007

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Recent advances in the understanding of stem cell mobilization, cell-matrix interaction, and biodistribution have enabled the development of new therapeutic strategies (1, 2). Although locally transplanted bone marrow-derived stromal cells (BMSC) 2 have already been used clinically (3-5), less invasive routes of BMSC transplantation have become the focus of recent attention (6 -8). In fact, several clinical applications now use intravenous administration of genetically engineered BMSC either as an ideal vehicle for gene transfer or as a platform for the systemic delivery of therapeutic recombinant proteins in vivo (6,9,10). This implies that these circulating, systemically infused cells must respond to serum-derived cues that direct their ultimate biodistribution. The molecular players regulating cellular mobilization, chemotaxis, and cell survival of BMSC have received little attention.Among the mediators known to exert potent cellular chemotactic effects, sphingosine 1-phosphate (S1P) is one of the most important bioactive lysophospholipids secreted in blood plasma either upon platelet activation (11) or from brain tumor-derived glioma cells (12). In fact, we have demonstrated that BMSC chemotaxis was very strong in response to S1P (13) and required reorganization of the actin cytoskeleton and remodeling of the extracellular matrix (ECM) through a complex, cooperative signal transduction network involving cell surface matrix metalloproteinase (MMP) activity (14). Currently, the molecular characterization and the nature of that MMP, regulating both BMSC chemotaxis and interaction with the ECM protein microenvironment, remain poorly understood. Recently, we highlighted functional cross-talk between the membrane type-1 MMP (MT1-MMP) and the S1P receptor EDG-1-mediated signaling in BMSC chemotaxis (13). Interestingly, aside from its classical role in ECM proteolysis, MT1-MMP is also involved in transducing crucial intracellular signaling that may control several processes related to BMSC mobilization and cell survival (13)(14)(15)(16).Given that impaired chemotaxis was recently observed in bone marrow cells isolated from a microsomal glucose 6-phos-* This work was supported by a grant of the Natural Sciences and EngineeringResearch Council of Canada (NSERC) (to B. A.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 2 The abbreviations used are: BMSC, bone marrow-derived stromal cell(s); ConA, concanavalin A; ECM, extracellular matrix; G6P, glucose 6-phosphate; G6Pase, glucose-6-phosphatase; G6PT, G6P transporter; MMP, matrix metalloproteinase; MT1-MMP, membrane type-1 MMP; PI, propidium iodine; siRNA, small interfering RNA; S1P, sphingosine 1-phosphate; PBS, phosphate-buffered saline; Wt, wild type; GFP, green fluorescent protein.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

MT1-MMP Down-regulates the Glucose 6-Phosphate Transporter Expression in Marrow Stromal Cells

Currie¹,

Fortier²,

Sina³

et al. 2007

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

PF‐8380 and Closely Related Analogs: Synthesis and Structure–Activity Relationship towards Autotaxin Inhibition and Glioma Cell Viability

St-Coeur

Ferguson

Morin

et al. 2013

Archiv der Pharmazie

View full text Add to dashboard Cite

A series of PF-8380 analogs, a recently developed autotaxin inhibitor, was explored. Inhibition of autotaxin by these analogs, as well as by all PF-8380 synthetic intermediates, shows the importance of meta-dichlorobenzyl and benzo[d]oxazol-2(3H)-one fragments. However, analogs 8 and 9, bearing only the benzo[d]oxazol-2(3H)-one moiety, are more cytotoxic on the LN229 glioblastoma cell line than PF-8380 and temozolomide (TMZ).

show abstract

IL‐1β microenvironment promotes proliferation, migration, and invasion of human glioma cells

Hurmath

Ramaswamy

Nandakumar

2014

Cell Biology International

View full text Add to dashboard Cite

Among the primary brain tumors, glioblastoma is the most common and severe. Glioblastoma have poor prognosis because of their highly diffusive growth pattern and invasion into surrounding brain tissue. Human glioblastoma cells overexpress interleukin-1β (IL-1β) and also the levels of IL-1β in U87MG glioma cells are significantly higher than in U373, T98G, A172 glioma cell lines. Malignant tumors are characterized by unlimited proliferation, migration and invasion. This study examines the effect of IL-1β microenvironment on proliferation, migration and invasion in human glioma cell line U87MG that expresses wild-type p53 protein, and U251MG which expresses mutant p53. Proliferation was investigated by MTT assay, migration by wound-healing migration assay and invasion by in vitro transwell Matrigel invasion assay. An IL-1β microenvironment significantly increased migration and invasion of both wild-type and mutant p53 expressing glioma cells, but significantly increased proliferation only in U87MG glioma cells. These effects were inhibited by IL-1 receptor antagonist (IL-1Ra), thus giving evidence that an IL-1β milieu promotes glioma cell migration, invasion, and proliferation.

show abstract

The chemopreventive properties of chlorogenic acid reveal a potential new role for the microsomal glucose-6-phosphate translocase in brain tumor progression

Cited by 131 publications

References 59 publications

MT1-MMP Down-regulates the Glucose 6-Phosphate Transporter Expression in Marrow Stromal Cells

MT1-MMP Down-regulates the Glucose 6-Phosphate Transporter Expression in Marrow Stromal Cells

PF‐8380 and Closely Related Analogs: Synthesis and Structure–Activity Relationship towards Autotaxin Inhibition and Glioma Cell Viability

IL‐1β microenvironment promotes proliferation, migration, and invasion of human glioma cells

Contact Info

Product

Resources

About