The chemotype core collection of genus Nicotiana

Abstract

SUMMARY Sustainable production of chemicals and improving these biosources by engineering metabolic pathways to create efficient plant‐based biofactories relies on the knowledge of available chemical/biosynthetic diversity present in the plant. Nicotiana species are well known for their amenability towards transformation and other new plant breeding techniques. The genus Nicotiana is primarily known through Nicotiana tabacum L., the source of tobacco leaves and all respective tobacco products. Due to the prevalence of the latter, N. tabacum and related Nicotiana species are one of the most extensively studied plants. The majority of studies focused solely on N. tabacum or other individual species for chemotyping. The present study analysed a diversity panel including 17 Nicotiana species and six accessions of Nicotiana benthamiana and created a data set that effectively represents the chemotype core collection of the genus Nicotiana. The utilisation of several analytical platforms and previously published libraries/databases enabled the identification and measurement of over 360 metabolites of a wide range of chemical classes as well as thousands of unknowns with dedicated spectral and chromatographic properties.

A comprehensive metabolomics and lipidomics atlas for the legumes common bean, chickpea, lentil and lupin

“…During the last decades, metabolomics techniques became a powerful tool in assessing metabolite levels in a wide range of populations among all kingdoms of life (Bar et al, 2020;Hall et al, 2002;Sebastiana et al, 2021), provided necessary precautions are taken (Alseekh, Aharoni, et al, 2021). The approach has been extensively defined (Alseekh & Fernie, 2018;Fiehn, 2002;Saito & Matsuda, 2010) and used to unravel various metabolic fingerprints among plant species, e.g., tobacco (Nicotiana benthamiana) (Drapal et al, 2022), tomato (Solanum lycopersicum) (Tohge & Fernie, 2015;Zhu et al, 2018), rice (Oryza sativa) (Gong et al, 2013) and maize (Zea mays) (Wen et al, 2014). In the field of food science, metabolomics is used to determine food quality and authenticity (Cubero-Leon et al, 2014).…”

Dually biofortified cisgenic tomatoes with increased flavonoids and branched‐chain amino acids content

“…tomatoes in this case), an approach that could help to identify any suspicious, unintended deviation from what is generally considered as safe in the metabolic composition of the same range of product. In this regard, the availability of increasingly complete pan‐metabolomes from different crop species is a highly valuable resource (Drapal et al ., 2022; Enfissi et al ., 2021). In our view, strong unintended deviations are especially unlikely to occur using cisgenic breeding approaches.…”

Evolutionary metabolomics of specialized metabolism diversification in the genusNicotianahighlights allopolyploidy-mediated innovations inN-acylnornicotine metabolism

“…In terms of structural information, rhe SM metabolic cartography generated in this study goes far beyond to a recently published chemotypic classification of the Nicotiana genus which mostly consisted in the dereplication of primary metabolites such as steroids and only a few SMs ( 51 ). In our opinion, this data platform and our SM cartography provide complementary views on the metabolic diversity of this genus.…”