The chlorophyll fluorescence ratio F690/F730 in leaves of different chlorophyll content

Abstract

The red laser-induced chlorophyll-fluorescence induction kinetics of predarkened leaf samples were registered simultaneously in the 690 and 730 nm regions i.e., in the region of the two chlorophyll fluorescence emission maxima. From the induction kinetics the chlorophyll fluorescence ratio F690/F730 was calculated. The ratio F690/F730 shows to be dependent on the chlorophyll content of leaves. It is significantly higher in needles of damaged spruces (values of 0.45-0.9) than in normal green needles of healthy trees (values of 0.35-0.5). During development and greening of maple leaves the ratio F690/F730 decreases with increasing chlorophyll content. Determination of the ratio F690/F730 can be a suitable method of monitoring changes in chlorophyll content in a non-destructive way in the same leaves during development or the yellowish-green discolouration of needles of damaged spruces in the Black Forest with the typical tree decline symptoms.

Fluorescence Emission Spectrum of Elodea Leaves Exposed to Nanoparticles

“…In more cases the peak of F 735 disappears. As stated above the ratio F690/F735 is inversely correlated to the chlorophyll content and can be used as a non-destructive determination method of the chlorophyll content [9] [10]. It is well known that when photosynthetic events related to biochemical or physiological processes are inhibited, the yield and kinetics of dissipated fluo-rescence are significantly changed [13].…”

“…The red chlorophyll fluorescence emission spectra are characterized by two maxima in the 690 and 735 nm regions. The ratio of F690/F735 is inversely correlated to the chlorophyll content and can be used as a live determination method of the chlorophyll content [9] [10]. It can also be applied to detect short-term and long-term stress effect on plants [11].…”

Water Deficit and Salt Stress Diagnosis Through LED Induced Chlorophyll Fluorescence Analysis in Jatropha curcas L.

“…The re-absorption is caused by the overlapping of the short-wavelength range of Chl fluorescence emission spectra with the long-wavelength range of the Chl absorption spectrum. As demonstrated by Lichtenthaler and coworkers [18], the Fr emission is much more affected by the reabsorption than the FFr leading to the fluorescence ratio Fr/ FFr decrease with increasing Chl content. The simultaneous measure of chlorophyll fluorescence in both red and far-red spectral region allows then the approximate determination of the Chl content of the leaves in a non-destructive way using the ChlF ratio [2,19].…”

Abiotic Stress Diagnosis via Laser Induced Chlorophyll Fluorescence Analysis in Plants for Biofuel

“…The Fr emission is much more affected by the reabsorption than the FFr, leading to the fluorescence ratio Fr/FFr decrease with increasing Chl content (Gitelson et al, 1998). The simultaneous measure of chlorophyll fluorescence in both red and far-red spectral region allows then the approximate determination of the Chl content of the leaves in a non-destructive way using the ChlF ratios Lichtenthaler et al, 1990). In Fig.…”