The chloroplast GATA-motif of Mahonia bealei participates in alkaloid-mediated photosystem inhibition during dark to light transition

Chen, Jiaqi; Jiang, Tianfu; Jiang, Jiajun; Deng, Linfang; Liu, Yiting; Zhong, Zhuoheng; Fu, Hongwei; Yang, Bingxian; Zhang, Lin

doi:10.1016/j.jplph.2022.153894

Cited by 5 publications

(3 citation statements)

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“…6), supporting its possible role in photosynthesis. In this study, the promoter element analysis was performed for 2000 bp upstream of GmRPI2, which contains a large number of light-responsive elements, among which GATA-motif and G-Box are mainly involved in light response, efficient and tissue-specific expression in plants (Chen et al 2023, Kobayashi et al 2012; by understanding the regulatory characteristics of the promoter and analyzing the promoter activity, we further suggest that this gene is related to photosynthesis, and we will further verify this conjecture through experiments.…”

Section: Discussionmentioning

confidence: 97%

Cloning and functional study of <i>GmRPI2</i>, which is the critical gene of photosynthesis in soybean

Sun

Wang

et al. 2023

Breed. Sci.

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Light provides energy for photosynthesis and is also an important environmental signal that regulates plant growth and development. Ribose-5-phosphate isomerase plays a crucial role in photosynthesis. However, ribose-5-phosphate isomerase has yet to be studied in soybean photosynthesis. To understand the biological function of GmRPI2, in this study, GmRPI2 was cloned, plant overexpression vectors and gene editing vectors were successfully constructed, and transformed into recipient soybean JN74 using the Agrobacteriummediated method. Using qRT-PCR, we analyzed that GmRPI2 gene expression was highest in leaves, second highest in roots, and lowest in stems. Promoter analysis revealed the presence of multiple cis-acting elements related to light response in the promoter region of GmRPI2. Compared with the control soybean plants, the net photosynthetic rate and transpiration rate of the overexpression lines were higher than those of the control and gene editing lines, while the intercellular CO 2 concentration was significantly lower than that of the control and gene editing lines; the total chlorophyll, chlorophyll a, chlorophyll b contents and soluble sugar contents of the overexpression plants were significantly higher than those of the recipient and editing plants, indicating that the GmRPI2 gene can increase The GmRPI2 gene can increase the photosynthetic capacity of soybean plants, providing a theoretical basis and genetic resources for improving soybean yield by regulating photosynthetic efficiency.

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Section: Discussionmentioning

confidence: 97%

Cloning and functional study of <i>GmRPI2</i>, which is the critical gene of photosynthesis in soybean

Sun

Wang

et al. 2023

Breed. Sci.

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“…AE-box and ATCT-motif are present within the 130-bp fragment of the D2-AtPolλ promoter and regulate the light-mediated activity of the promoter via binding of specific trans -acting factors [ 71 ]. Wide- and narrow-leaf plants have distinct frequencies of GATA-motif elements in chloroplast genomes [ 72 ]. Indeed, Vvi-MIR399f and Vvi-MIR399g were promptly increased by low and high light stress, implying that Vvi-miR399f and Vvi-miR399g can possibly function as modulators of light-induced signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Identification and Analysis of the MIR399 Gene Family in Grapevine Reveal Their Potential Functions in Abiotic Stress

Liu,

Ren,

Sun

et al. 2024

IJMS

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MiR399 plays an important role in plant growth and development. The objective of the present study was to elucidate the evolutionary characteristics of the MIR399 gene family in grapevine and investigate its role in stress response. To comprehensively investigate the functions of miR399 in grapevine, nine members of the Vvi-MIR399 family were identified based on the genome, using a miRBase database search, located on four chromosomes (Chr 2, Chr 10, Chr 15, and Chr 16). The lengths of the Vvi-miR399 precursor sequences ranged from 82 to 122 nt and they formed stable stem–loop structures, indicating that they could produce microRNAs (miRNAs). Furthermore, our results suggested that the 2 to 20 nt region of miR399 mature sequences were relatively conserved among family members. Phylogenetic analysis revealed that the Vvi-MIR399 members of dicots (Arabidopsis, tomato, and sweet orange) and monocots (rice and grapevine) could be divided into three clades, and most of the Vvi-MIR399s were closely related to sweet orange in dicots. Promoter analysis of Vvi-MIR399s showed that the majority of the predicted cis-elements were related to stress response. A total of 66.7% (6/9) of the Vvi-MIR399 promoters harbored drought, GA, and SA response elements, and 44.4% (4/9) of the Vvi-MIRR399 promoters also presented elements involved in ABA and MeJA response. The expression trend of Vvi-MIR399s was consistent in different tissues, with the lowest expression level in mature and young fruits and the highest expression level in stems and young leaves. However, nine Vvi-MIR399s and four target genes showed different expression patterns when exposed to low light, high light, heat, cold, drought, and salt stress. Interestingly, a putative target of Vvi-MIR399 targeted multiple genes; for example, seven Vvi-MIR399s simultaneously targeted VIT_213s0067g03280.1. Furthermore, overexpression of Vvi_MIR399e and Vvi_MIR399f in Arabidopsis enhanced tolerance to drought compared with wild-type (WT). In contrast, the survival rate of Vvi_MIR399d-overexpressed plants were zero after drought stress. In conclusion, Vvi-MIR399e and Vvi-MIR399f, which are related to drought tolerance in grapevine, provide candidate genes for future drought resistance breeding.

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“…Cis ‐acting elements play an important role in gene transcription and expression (Zheng et al., 2021). Previous studies have shown that many types of cis ‐acting elements were present in the WRKY promoters of nine species, including ABREs (Nakashima & Yamaguchi‐Shinozaki, 2013), GA response elements (P‐box) (Liu et al., 2016), jasmonic acid (JA) response elements (MeJA responsive) (Rabara et al., 2013), SA response elements (TCA‐element) (Zheng et al., 2021), temperature response elements (LTR) (Wu et al., 2019), light response elements (GATA motif‐containing) (Chen et al., 2022), defense responsive elements (TC‐rich repeats) (Zheng et al., 2021), wound‐responsive elements (WUN motif‐containing) (He et al., 2021) and drought response elements (MBSs) (Zheng et al., 2021). The presence of many cis ‐acting elements mediating hormone and stress responses indicated that these genes are involved in different biological processes.…”

Section: Discussionmentioning

confidence: 99%

Identification and expression analysis of WRKY superfamily genes under lychee downy blight stress in lychee

Liu,

Jiang,

Wen

et al. 2023

Agronomy Journal

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WRKY proteins comprise a large family of transcription factors that play important roles in plant growth, development, and responses to stress, but little information is available about these genes in lychee (Litchi chinensis Sonn.). Despite being a popular fruit worldwide, lychee is highly vulnerable to lychee downy blight (LDB), a major disease affecting lychee. Based on lychee whole‐genome data, a total of 50 LcWRKY genes were identified across 15 chromosomes. According to a conserved domain and phylogenetic analysis, these 50 LcWRKYs were classified into three distinct subfamilies. Gene structure analysis revealed the presence of 2‐11 exons in the LcWRKYs. Cis‐acting regulatory element analysis identified many stress‐response elements in LcWRKY promoters. Moreover, a comprehensive two‐year investigation involving the screening of a lychee natural population led to the identification of Guiwei (GW) and Yurong 1 (YR1) as displaying stable susceptibility and resistance against LDB, respectively. Furthermore, expression patterns indicated that LcWRKY13, LcWRKY17, and LcWRKY34 were significantly upregulated in response to LDB induction, while LDB suppressed the expression of LcWRKY11. These findings suggest that these four genes may play a crucial role in lychee resistance to LDB. The present study has provided a foundation for future investigations of LcWRKY gene function in lychee.This article is protected by copyright. All rights reserved

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The chloroplast GATA-motif of Mahonia bealei participates in alkaloid-mediated photosystem inhibition during dark to light transition

Cited by 5 publications

References 54 publications

Cloning and functional study of <i>GmRPI2</i>, which is the critical gene of photosynthesis in soybean

Cloning and functional study of <i>GmRPI2</i>, which is the critical gene of photosynthesis in soybean

Identification and Analysis of the MIR399 Gene Family in Grapevine Reveal Their Potential Functions in Abiotic Stress

Identification and expression analysis of WRKY superfamily genes under lychee downy blight stress in lychee

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