The chloroplast lumen and stromal proteomes of <i>Arabidopsis thaliana</i> show differential sensitivity to short‐ and long‐term exposure to low temperature

Goulas, Estelle; Schubert, Maria; Kieselbach, Thomas; Kleczkowski, Leszek A.; Gardeström, Per; Schröder, Wolfgang P.; Hurry, Vaughan

doi:10.1111/j.1365-313x.2006.02821.x

Cited by 213 publications

(172 citation statements)

References 90 publications

Supporting

Mentioning

161

Contrasting

Unclassified

Order By: Relevance

“…Washing and elution of proteins coimmunoprecipitated with PSB33 were performed according to the manufacturer's instructions (Pierce Crosslink Magnetic Co-IP Kit 88805). The eluted proteins were separated on a 15% (v/v) polyacrylamide gel and stained with silver as described (Goulas et al, 2006). In-gel digestion of selected protein bands was performed as described (Fristedt and Vener, 2011).…”

Section: Antibody Productionmentioning

confidence: 99%

PHOTOSYSTEM II PROTEIN33, a Protein Conserved in the Plastid Lineage, Is Associated with the Chloroplast Thylakoid Membrane and Provides Stability to Photosystem II Supercomplexes in Arabidopsis

et al. 2014

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-2594-509X (S.S.M.); 0000-0001-6974-9587 (R.L.L.).Photosystem II (PSII) is a multiprotein complex that catalyzes the light-driven water-splitting reactions of oxygenic photosynthesis. Light absorption by PSII leads to the production of excited states and reactive oxygen species that can cause damage to this complex. Here, we describe Arabidopsis (Arabidopsis thaliana) At1g71500, which encodes a previously uncharacterized protein that is a PSII auxiliary core protein and hence is named PHOTOSYSTEM II PROTEIN33 (PSB33). We present evidence that PSB33 functions in the maintenance of PSII-light-harvesting complex II (LHCII) supercomplex organization. PSB33 encodes a protein with a chloroplast transit peptide and one transmembrane segment. In silico analysis of PSB33 revealed a light-harvesting complexbinding motif within the transmembrane segment and a large surface-exposed head domain. Biochemical analysis of PSII complexes further indicates that PSB33 is an integral membrane protein located in the vicinity of LHCII and the PSII CP43 reaction center protein. Phenotypic characterization of mutants lacking PSB33 revealed reduced amounts of PSII-LHCII supercomplexes, very low state transition, and a lower capacity for nonphotochemical quenching, leading to increased photosensitivity in the mutant plants under light stress. Taken together, these results suggest a role for PSB33 in regulating and optimizing photosynthesis in response to changing light levels.

show abstract

Section: Antibody Productionmentioning

confidence: 99%

PHOTOSYSTEM II PROTEIN33, a Protein Conserved in the Plastid Lineage, Is Associated with the Chloroplast Thylakoid Membrane and Provides Stability to Photosystem II Supercomplexes in Arabidopsis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…At1g23740 protein has a predicted transit peptide in the Nterminal region and has been identified as a stromal protein in chloroplasts by two independent proteomic analyses (23,24). Accordingly, we concluded that AtAOR was the chloroplastlocalizing AOR.…”

Section: Purification Of Acrolein-reducing Enzyme From Cucumbermentioning

confidence: 80%

NADPH-dependent Reductases Involved in the Detoxification of Reactive Carbonyls in Plants

Yamauchi¹,

Hasegawa²,

Taninaka

et al. 2011

Journal of Biological Chemistry

152

117

View full text Add to dashboard Cite

Reactive carbonyls, especially ␣,␤-unsaturated carbonyls produced through lipid peroxidation, damage biomolecules such as proteins and nucleotides; elimination of these carbonyls is therefore essential for maintaining cellular homeostasis. In this study, we focused on an NADPH-dependent detoxification of reactive carbonyls in plants and explored the enzyme system involved in this detoxification process. Using acrolein (CH 2 ‫؍‬ CHCHO) as a model ␣,␤-unsaturated carbonyl, we purified a predominant NADPH-dependent acrolein-reducing enzyme from cucumber leaves, and we identified the enzyme as an alkenal/one oxidoreductase (AOR) catalyzing reduction of an ␣,␤-unsaturated bond. Cloning of cDNA encoding AORs revealed that cucumber contains two distinct AORs, chloroplastic AOR and cytosolic AOR. Homologs of cucumber AORs were found among various plant species, including Arabidopsis, and we confirmed that a homolog of Arabidopsis (At1g23740) also had AOR activity. Phylogenetic analysis showed that these AORs belong to a novel class of AORs. They preferentially reduced ␣,␤-unsaturated ketones rather than ␣,␤-unsaturated aldehydes. Furthermore, we selected candidates of other classes of enzymes involved in NADPH-dependent reduction of carbonyls based on the bioinformatic information, and we found that an aldo-keto reductase (At2g37770) and aldehyde reductases (At1g54870 and At3g04000) were implicated in the reduction of an aldehyde group of saturated aldehydes and methylglyoxal as well as ␣,␤-unsaturated aldehydes in chloroplasts. These results suggest that different classes of NADPH-dependent reductases cooperatively contribute to the detoxification of reactive carbonyls.When plants are subjected to abiotic and/or biotic stresses, oxidative stress often results; this leads to the production of reactive oxygen species, which damage biomolecules such as proteins and lipids. More than half of the fatty acids found in the membranes of chloroplasts and mitochondria, two of the most highly oxidative organelles, are linoleic and linolenic acid. Because linoleic and linolenic acid are sources of many short chain carbonyls through their peroxidation (1), biomolecules in both types of organelles are challenged by the toxicity of reactive compounds, including ␣,␤-unsaturated carbonyls, which are involved in the pathophysiological effects associated with oxidative stress in cells and tissues (2). In fact, ␣,␤-unsaturated carbonyls from peroxidized polyunsaturated fatty acids cause loss of functions in mitochondria (3); chloroplasts have recently been shown to be a major production center of reactive aldehydes (4), and photosynthetic functions are highly sensitive to ␣,␤-unsaturated carbonyls (5-7).The high reactivity of ␣,␤-unsaturated carbonyls is due to the ability of their ␣,␤-unsaturated bonds to form Michael adducts with thiol and amino groups in biomolecules; in the case of ␣,␤-unsaturated aldehydes, the aldehyde group also contributes to reactivity through the formation of Schiff base adducts with amino groups. Thus the targe...

show abstract

“…The GST proteins have been shown to respond to various stresses such as HL, cold, and drought (Wagner et al, 2002;Seki et al, 2003;Goulas et al, 2006). After exposure to HL, six different GST genes were induced, and four of them were induced in both HL and high intensity BL (Supplemental Tables S1 and S2).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance

et al. 2007

View full text Add to dashboard Cite

Exposure to high irradiance results in dramatic changes in nuclear gene expression in plants. However, little is known about the mechanisms by which changes in irradiance are sensed and how the information is transduced to the nucleus to initiate the genetic response. To investigate whether the photoreceptors are involved in the response to high irradiance, we analyzed expression of EARLY LIGHT-INDUCIBLE PROTEIN1 (ELIP1), ELIP2, ASCORBATE PEROXIDASE2 (APX2), and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN2.4 (LHCB2.4) in the phytochrome A (phyA), phyB, cryptochrome1 (cry1), and cry2 photoreceptor mutants and long hypocotyl5 (hy5) and HY5 homolog (hyh) transcription factor mutants. Following exposure to high intensity white light for 3 h (1,000 mmol quanta m 22 s 21 ) expression of ELIP1/2 and APX2 was strongly induced and LHCB2.4 expression repressed in wild type. The cry1 and hy5 mutants showed specific misregulation of ELIP1/2, and we show that the induction of ELIP1/2 expression is mediated via CRY1 in a blue light intensity-dependent manner. Furthermore, using the Affymetrix Arabidopsis (Arabidopsis thaliana) 24 K Gene-Chip, we showed that 77 of the high lightresponsive genes are regulated via CRY1, and 26 of those genes were also HY5 dependent. As a consequence of the misregulation of these genes, the cry1 mutant displayed a high irradiance-sensitive phenotype with significant photoinactivation of photosystem II, indicated by reduced maximal fluorescence ratio. Thus, we describe a novel function of CRY1 in mediating plant responses to high irradiances that is essential to the induction of photoprotective mechanisms. This indicates that high irradiance can be sensed in a chloroplast-independent manner by a cytosolic/nucleic component.

show abstract

The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short‐ and long‐term exposure to low temperature

Cited by 213 publications

References 90 publications

PHOTOSYSTEM II PROTEIN33, a Protein Conserved in the Plastid Lineage, Is Associated with the Chloroplast Thylakoid Membrane and Provides Stability to Photosystem II Supercomplexes in Arabidopsis

PHOTOSYSTEM II PROTEIN33, a Protein Conserved in the Plastid Lineage, Is Associated with the Chloroplast Thylakoid Membrane and Provides Stability to Photosystem II Supercomplexes in Arabidopsis

NADPH-dependent Reductases Involved in the Detoxification of Reactive Carbonyls in Plants

Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance

Contact Info

Product

Resources

About