The choice of anti-LEDGF/DFS70 assay matters: a comparative study of six assays

Troelnikov, Alexander; Hender, Lauren; Lester, Susan C.; Gordon, Thomas P.; Hughes, Tiffany F.; Beroukas, Dimitra

doi:10.1016/j.pathol.2022.07.010

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…11,12 Current data linked with clinical-serological correlations revealed some controversial findings. 3 Some authors claim that patients with CTDs are characterized by a low incidence of the DFS pattern observed in IIF with circulating anti-DFS70 antibodies, while others state that the presence of these antibodies is not a sufficently reliable factor to clearly exclude a CTD. 11 Still other authors state that anti-DFS70 autoantibodies are present at a very low frequency in CTDs compared with other inflammatory diseases or healthy populations.…”

Section: Anti-dfs70 Antibodiesmentioning

confidence: 99%

“…However, there is considerable interassay variability for these autoantibodies in clinical laboratories. 3 Despite their historical name, ANAs are directed against a wide spectrum of intracellular antigens, which include both the structures of the cell nucleus and the cytoplasm. 4 The initial identification of ANAs by IIF remains the gold standard in the immunodiagnosis of CTDs.…”

Section: Introductionmentioning

confidence: 99%

“…However, there is considerable interassay variability for these autoantibodies in clinical laboratories. 3 …”

Section: Introductionmentioning

confidence: 99%

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Dense fine speckled nuclear immunofluorescence: A mildly reassuring antinuclear antibody pattern meriting consideration

Fijałkowska,

Schwartz,

Woźniacka

2023

Immunity Inflam & Disease

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IntroductionAntinuclear antibodies (ANAs) are regarded as a hallmark of connective tissue diseases (CTDs) and play a key role in their diagnosis, but the value of some particular antibodies in management of patients and the disease prognosis is controversial. The mechanism underlying the production of ANAs in CTDs, other chronic inflammatory conditions and even in healthy people, is not completely elucidated. Anti‐DFS70 antibodies connected with the dense fine speckled autoantigen of 70 kD, known as the lens epithelium‐derived growth factor p75, are a subgroup of ANAs. Their presence and coexistence with other antibodies and their clinical significance are the matter of debate.MethodsBased on literature data, the authors focused on current knowledge explaining the role of anti‐DFS70 antibodies in selected CTDs.ResultsHowever, the literature data is ambiguous and does not fully support the validity of the anti‐DFS70 assay for a specific CTD diagnosis. Most researchers claim that the presence of anti‐DFS70 as the only one usually exclude the diagnosis of CTD. Nevertheless, its coexistence with other ANAs is not an excluding factor but has predictive value due to more favorable course of CTD. Such situations may also suggest an enhanced risk of the development of a CTD in the future.ConclusionsAlthough more studies are needed in this field, it seems reasonable to ascertain the presence of anti‐DFS70 in routine clinical practice.

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Section: Anti-dfs70 Antibodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dense fine speckled nuclear immunofluorescence: A mildly reassuring antinuclear antibody pattern meriting consideration

Fijałkowska,

Schwartz,

Woźniacka

2023

Immunity Inflam & Disease

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A novel magnetic bead-based immunoprecipitation method for anti-dense fine speckled 70 antibodies

Chen¹,

Wei²,

Yang³

et al. 2023

Pathology

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DFS70 Autoantibodies: Clinical Utility in Antinuclear Antibody Testing

Bossuyt

2023

Clinical Chemistry

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Background Screening for antinuclear antibodies (ANA) by indirect immunofluorescence (IIF) on HEp-2 cells is helpful for the diagnosis and classification of ANA-associated rheumatic diseases, including systemic lupus erythematosus, Sjögren syndrome, mixed connective tissue disease, systemic sclerosis, and inflammatory myopathies. The dense fine speckled (DFS) pattern is a special HEp-2 IIF pattern (produced by anti-DFS70 antibodies) because it is not associated with a specific medical condition and therefore can obfuscate interpretation. Content In this paper, detection methods for and clinical associations of anti-DFS70 antibodies are reviewed. Summary The target antigen of the antibodies that cause the DFS pattern is a 70 kDa protein (DFS70). Commercial methods that detect antibodies to full-length or truncated DFS70 are available for use in clinical laboratories (ELISA, chemiluminescence, dot/line blot). Anti-DFS70 can be found in (apparently) healthy individuals (with a higher frequency in young individuals and in females), in several (inflammatory) conditions and in malignancy. There is no clinical association that is well-established. Special attention (and critical reflection) is given to the observation that monospecific anti-DFS70 (i.e., in the absence of antibodies that are linked to ANA-associated rheumatic diseases) is rarely found in ANA-associated rheumatic diseases.

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The choice of anti-LEDGF/DFS70 assay matters: a comparative study of six assays

Cited by 3 publications

References 32 publications

Dense fine speckled nuclear immunofluorescence: A mildly reassuring antinuclear antibody pattern meriting consideration

Dense fine speckled nuclear immunofluorescence: A mildly reassuring antinuclear antibody pattern meriting consideration

A novel magnetic bead-based immunoprecipitation method for anti-dense fine speckled 70 antibodies

DFS70 Autoantibodies: Clinical Utility in Antinuclear Antibody Testing

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