The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues

Merkl, M.; Ertl, Reinhard; Handschuh, Stephan; Aurich, Christine; Schäfer-Somi, Sabine

doi:10.1016/j.theriogenology.2015.11.019

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…Therefore blockade at different physiological statuses may lead to different results. In a former study [11], we detected expression of ABCA1 in canine testicular and epididymal tissue and spermatozoa membranes as has been done in human and stallion testicles, and spermatozoa [9,25]. In canine spermatozoa, ABCA1 was mainly assessed in the tails of spermatozoa from the cauda epididymidis.…”

Section: Discussionmentioning

confidence: 99%

The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa

Schäfer-Somi

Budik

2020

Acta Vet Scand

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Background: The ATP binding cassette (ABC) transporters participate in the cholesterol and phospholipid transport within and through cell membranes of many cells including spermatozoa. Cholesterol efflux is important for capacitation of spermatozoa. ABCA1 expression has been assessed in canine spermatozoa previously but its role in capacitation still has to be determined. The aim of the study was to test whether inhibition of ABCA1 (1) decreases capacitation in ejaculated and epididymal canine sperm samples and (2) decreases cholesterol efflux in the same samples. Twenty-one ejaculates and sperm from 22 epididymal tails were collected from healthy dogs. Motility was measured by CASA and viability assessed after staining with SYBR-14/PI. Samples from ejaculated sperm and sperm from epididymal tails were aliquoted. One part was incubated with the ABCA1 inhibitor probucol, the other served as a negative control. In all samples, capacitation was evaluated by chlortetracyclin (CTC) assay and cholesterol was measured by cholesterol efflux assay and colorimetric enzymatic assay. Results: In ejaculated sperm, blockade of ABCA1 with 100 µM of probucol/mL of sample resulted in a significantly higher percentage of uncapacitated and acrosome reacted spermatozoa (P < 0.001 and P = 0.031), capacitation was significantly decreased (35% in probucol samples vs 54.2% in controls, P < 0.001). In probucol inhibited sperm samples from epididymal tails, the percentage of capacitated spermatozoa did not differ between groups but the percentage of acrosome reacted spermatozoa increased significantly (P = 0.014). The cholesterol measurement revealed significantly lower cholesterol concentration in the probucol group when compared to the controls (P = 0.035), however only in ejaculated sperm samples. Conclusions: CTC assay and cholesterol measurement revealed significant differences between groups; we conclude that inhibition of ABCA1 significantly decreased capacitation and cholesterol efflux in ejaculated canine spermatozoa. The inhibition was not complete but ABCA1 is supposed to contribute to capacitation in canine ejaculated spermatozoa. ABCA1 is probably not important for capacitation of epididymal spermatozoa but might exert other functions during spermatozoa ripening.

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Section: Discussionmentioning

confidence: 99%

The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa

Schäfer-Somi

Budik

2020

Acta Vet Scand

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“…It is known that there may be variations in the transport of cholesterol towards the epididymis under certain conditions; for example, during spermatogenesis, cholesterol transport is increased [32]. The mechanisms that regulate the transport of cholesterol to the epididymis are not fully understood, however it is known that the ABCA1 transporter is the main transporter of cholesterol in this tissue [33] so DG and ATV could modify the expression of this protein and therefore change the amount of cholesterol that is concentrated in epididymis. In the kidney, a significant difference was observed in the EZT group compared to HD group.…”

Section: Discussionmentioning

confidence: 99%

“…In the kidney, a significant difference was observed in the EZT group compared to HD group. EZT increases the concentration of cholesterol in the kidney compared to HD, but shows similar concentrations to ND, so apparently with high concentrations of cholesterol it is distributed mainly to the liver, suggesting that under these conditions EZT could have a role in the regulation of cholesterol in the kidney [32,33].…”

Section: Discussionmentioning

confidence: 99%

Combined Effect of Diosgenin Along with Ezetimibe or Atorvastatin on the Fate of Labelled Bile Acid and Cholesterol in Hypercholesterolemic Rats

Marín-Medina

Ruíz-Hidalgo

Blé-Castillo

et al. 2019

IJERPH

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We analyzed the effect of diosgenin, administered with atorvastatin or ezetimibe, on the fate of 3H(G)-taurocholic acid or 26-14C-cholesterol in hypercholesterolemic rats. Male Wistar rats received a hypercholesterolemic diet (HD), HD + atorvastatin (HD+ATV), HD + ezetimibe (HD+EZT), HD + diosgenin (HD+DG), HD+ATV+EZT, or HD+ATV+DG for 40 days. We also included a control normal group (ND). The labelled compounds were administered on day 30. The animals were placed in metabolic cages for daily feces collection. At day 40 the rats were sacrificed. Lipid extracts from blood, liver, spinal cord, testicles, kidneys, epididymis, intestine, and feces were analyzed for radioactivity. Cholesterol activity was the highest in the liver in HD rats. DG diminished one half of this activity in HD+DG and HD+ATV+DG groups in comparison with the HD group. HD+ATV rats showed four to almost ten-fold cholesterol activity in the spinal cord compared with the ND or HD rats. Fecal elimination of neutral steroids was approximately two-fold higher in the HD+DG and HD+ATV+DG groups. Taurocholic acid activity was four to ten-fold higher in HD+DG intestine as compared to the other experimental groups. Taurocholic activity in the liver of HD and HD+DG groups was two and a half higher than in ND. Our results show that the combination of DG and ATV induced the highest cholesterol reduction in the liver and other tissues.

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“…Naturschützern immer betonten Notwendigkeit der Praktikabilität des Naturschutzes verständlich". 204 Das Reichsnaturschutzgesetz galt in Österreich auch nach Ende des Zweiten Weltkriegs in allen neun Bundesländern weiter, solange diese nicht neue Naturschutzgesetze erließen. Dies tat wiederum Niederösterreich als erstes im Jahr 1952, das letzte Bundesland war die Steiermark 1976.…”

Section: Zum Schutz Des Edelweißunclassified

„aus dem Sternenhimmel hat es sein Modell geholt" Zu den Anfängen des Artenschutzrechts in Österreich.

Staudigl¹,

Staudigl-Ciechowicz²

2017

BRGOE

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The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues

Cited by 10 publications

References 44 publications

The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa

The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa

Combined Effect of Diosgenin Along with Ezetimibe or Atorvastatin on the Fate of Labelled Bile Acid and Cholesterol in Hypercholesterolemic Rats

„aus dem Sternenhimmel hat es sein Modell geholt" Zu den Anfängen des Artenschutzrechts in Österreich.

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