The Chorioallantoic Membrane Assay in Nanotoxicological Research—An Alternative for In Vivo Experimentation

Buhr, Christoph Raphael; Wiesmann, Nadine; Tanner, Rachel; Brieger, Jürgen; Eckrich, Jonas

doi:10.3390/nano10122328

Cited by 26 publications

(14 citation statements)

References 108 publications

(479 reference statements)

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“…For further biologic evaluation, PCCPMs were covalently labeled with DBCO-sulforhodamine B by alkyne-azide click chemistry and investigated in the in ovo in the chorioallantoic membrane assay (CAM assay). 76 The CAM assay has been previously described as well suited model for the evaluation of nanoparticle toxicity and the study of circulation times, as well as clearance of nanoparticles from the organism. 77 As illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Bauer

Eckrich²,

Wiesmann³

et al. 2021

J. Mater. Chem. B

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Section: Resultsmentioning

confidence: 99%

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Bauer

Eckrich²,

Wiesmann³

et al. 2021

J. Mater. Chem. B

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“…Therefore, the CAM test has already been used in different experimental settings, including wound healing, angiogenesis, cancer development, and toxicological research. 208 CAM research was employed to investigate both the toxicity and the angiogenic activity of AgNPs. Ag-functionalized materialexposed eggs revealed the antiangiogenic activities of a test material with minor blood vessels and lower capillary connectivity.…”

Section: Phagocytosis Assaymentioning

confidence: 99%

“…The highly vascularized CAM medium was used for examining the irritation potential of the testing material. Therefore, the CAM test has already been used in different experimental settings, including wound healing, angiogenesis, cancer development, and toxicological research . CAM research was employed to investigate both the toxicity and the angiogenic activity of AgNPs.…”

Section: Toxicity Studymentioning

confidence: 99%

Comprehensive Review on the Degradation Chemistry and Toxicity Studies of Functional Materials

Pagar

Musale

Pawar

et al. 2022

ACS Biomater. Sci. Eng.

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In recent decades there has been growing interest of material chemists in the successful development of functional materials for drug delivery, tissue engineering, imaging, diagnosis, theranostic, and other biomedical applications with advanced nanotechnology tools. The efficacy and safety of functional materials are determined by their pharmacological, toxicological, and immunogenic effects. It is essential to consider all degradation pathways of functional materials and to assess plausible intermediates and final products for quality control. This review provides a brief insight into chemical degradation mechanisms of functional materials like oxidation, photodegradation, and physical and enzymatic degradation. The intermediates and products of degradation were confirmed with analytical methods such as proton nuclear magnetic resonance (1H NMR), gel permeation chromatography (GPC), UV–vis spectroscopy (UV–vis), infrared spectroscopy (IR), differential scanning calorimetry (DSC), mass spectroscopy, and other sophisticated analytical methods. These analytical methods are also used for regulatory, quality control, and stability purposes in industry. The assessment of degradation is important to predetermine the behavior of functional materials in specific storage conditions and can be relevant to their behavior during in vivo applications. Another important aspect is the evaluation of the toxicity of functional materials. Toxicity can be accessed with various methods using in vitro, in vivo, ex vivo, and in silico models. In vitro cell culture methods are used to determine mitochondrial damage, reactive oxygen species, stress responses, and cellular toxicity. In vitro cellular toxicity can be measured by MTT assay, LDH leakage assay, and hemolysis. In vivo studies are performed using various animal models involving zebrafish, rodents (mice and rats), and nonhuman primates. Ex vivo studies are also used for efficacy and toxicity determinations of functional materials like ex vivo potency assay and precision-cut liver slice (PCLS) models. The in silico tools with computational simulations like quantitative structure–activity relationships (QSAR), pharmacokinetics (PK) and pharmacodynamics (PD), dose and time response, and quantitative cationic–activity relationships ((Q)CARs) are used for prediction of the toxicity of functional materials. In this review, we studied the principle methods used for degradation studies, different degradation pathways, and mechanisms of functional material degradation with prototype examples. We discuss toxicity assessments with different toxicity approaches used for estimation of the safety and efficacy of functional materials.

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“…Previous studies already proved that the chicken chorioallantoic membrane assay (CAM assay) is well suited for assessing the biocompatibility of nanomaterials [32], their fate within the organism, and hemocompatibility, as well as potential toxic effects [33]. Furthermore, the CAM is an excellent platform with which to study angiogenesis [34] and the angiogenetic activity of biomaterials [35,36].…”

Section: Chicken Chorioallantoic Membrane Assay (Cam-assay)mentioning

confidence: 99%

Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials

et al. 2021

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Due to the demographic change, medicine faces a growing demand for tissue engineering solutions and implants. Often, satisfying tissue regeneration is difficult to achieve especially when co-morbidities hamper the healing process. As a novel strategy, we propose the incorporation of zinc oxide nanoparticles (ZnO NPs) into biomaterials to improve tissue regeneration. Due to their wide range of biocompatibility and their antibacterial properties, ZnO NPs are already discussed for different medical applications. As there are versatile possibilities of modifying their form, size, and function, they are becoming increasingly attractive for tissue engineering. In our study, in addition to antibacterial effects of ZnO NPs, we show for the first time that ZnO NPs can foster the metabolic activity of fibroblasts as well as endothelial cells, both cell types being crucial for successful implant integration. With the gelatin sponge method performed on the chicken embryo’s chorioallantoic membrane (CAM), we furthermore confirmed the high biocompatibility of ZnO NPs. In summary, we found ZnO NPs to have very favorable properties for the modification of biomaterials. Here, incorporation of ZnO NPs could help to guide the tissue reaction and promote complication-free healing.

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The Chorioallantoic Membrane Assay in Nanotoxicological Research—An Alternative for In Vivo Experimentation

Cited by 26 publications

References 108 publications

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Comprehensive Review on the Degradation Chemistry and Toxicity Studies of Functional Materials

Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials

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