The Chriz–Z4 complex recruits JIL-1 to polytene chromosomes,a requirement for interband-specific phosphorylation of H3S10

Gan, Miao; Moebus, Selina; Eggert, Harald; Saumweber, Harald

doi:10.1007/s12038-011-9089-y

Cited by 31 publications

(33 citation statements)

References 33 publications

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“…3D-F). In addition to SU(HW), L(3)MBT interacts with a chromodomain protein, Chromator, that has also recently been shown to colocalize and cooperate with the BEAF-32 insulator (Giot et al 2003;Gan et al 2011). Indeed, using publicly available ChIP-chip data for Chromator in Drosophila Kc cells (Celniker et al 2009), aligned dCTCF sites also show an apparent enrichment for Chromator (Fig.…”

Section: Aligned Dctcf Sites Are Enriched For Cp190 Mod(mdg4) Isoformentioning

confidence: 94%

Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains

et al. 2012

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Several multiprotein DNA complexes capable of insulator activity have been identified in Drosophila melanogaster, yet only CTCF, a highly conserved zinc finger protein, and the transcription factor TFIIIC have been shown to function in mammals. CTCF is involved in diverse nuclear activities, and recent studies suggest that the proteins with which it associates and the DNA sequences that it targets may underlie these various roles. Here we show that the Drosophila homolog of CTCF (dCTCF) aligns in the genome with other Drosophila insulator proteins such as Suppressor of Hairy wing [SU(HW)] and Boundary Element Associated Factor of 32 kDa (BEAF-32) at the borders of H3K27me3 domains, which are also enriched for associated insulator proteins and additional cofactors. RNAi depletion of dCTCF and combinatorial knockdown of gene expression for other Drosophila insulator proteins leads to a reduction in H3K27me3 levels within repressed domains, suggesting that insulators are important for the maintenance of appropriate repressive chromatin structure in Polycomb (Pc) domains. These results shed new insights into the roles of insulators in chromatin domain organization and support recent models suggesting that insulators underlie interactions important for Pc-mediated repression. We reveal an important relationship between dCTCF and other Drosophila insulator proteins and speculate that vertebrate CTCF may also align with other nuclear proteins to accomplish similar functions.

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Section: Aligned Dctcf Sites Are Enriched For Cp190 Mod(mdg4) Isoformentioning

confidence: 94%

Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains

et al. 2012

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“…M1BP is a recently discovered zinc finger transcription factor that has been shown to orchestrate promoter-proximal pausing in a GAF-independent manner (Li and Gilmour 2013). BEAF-32 is one of the insulator-associated proteins identified in Drosophila (Zhao et al 1995), Chromator was initially identified as a mitotic spindle protein and later implicated in the regulation of chromosome structure through partial cooperation with BEAF-32 (Rath et al 2006;Gan et al 2011), and both of these proteins are enriched at the boundaries of physical chromosomal domains (Hou et al 2012;Sexton et al 2012). The factor with the highest differential enrichment for promoterbound genes in our screen was the tandem kinase JIL-1 (Supplemental Table S6), which has been shown previously to interact with Chromator (Rath et al 2006).…”

Section: Insulator Proteins and M1bp Are Enriched In The Promoter Regmentioning

confidence: 99%

Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation

et al. 2016

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The coordinated regulation of gene expression at the transcriptional level is fundamental to development and homeostasis. Inducible systems are invaluable when studying transcription because the regulatory process can be triggered instantaneously, allowing the tracking of ordered mechanistic events. Here, we use precision run-on sequencing (PRO-seq) to examine the genome-wide heat shock (HS) response in Drosophila and the function of two key transcription factors on the immediate transcription activation or repression of all genes regulated by HS. We identify the primary HS response genes and the rate-limiting steps in the transcription cycle that GAGA-associated factor (GAF) and HS factor (HSF) regulate. We demonstrate that GAF acts upstream of promoterproximally paused RNA polymerase II (Pol II) formation (likely at the step of chromatin opening) and that GAF-facilitated Pol II pausing is critical for HS activation. In contrast, HSF is dispensable for establishing or maintaining Pol II pausing but is critical for the release of paused Pol II into the gene body at a subset of highly activated genes. Additionally, HSF has no detectable role in the rapid HS repression of thousands of genes.

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“…We observed prominent binding of Chriz, Z4 (data not shown) and Jil-1, proteins that are typically found in decondensed chromatin as reported previously (Eggert et al, 2004;Gortchakov et al, 2005;Wang et al, 2001). Chriz and Jil-1 physically interact (Rath et al, 2006), and on Chriz knockdown both Jil-1 chromosomal binding and H3S10 phosphorylation are dramatically reduced, indicating that Chriz is required for chromosomal targeting of Jil-1 (Gan et al, 2011). Forced local recruitment of active Jil-1 kinase results in local chromatin decondensation (Deng et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Forced local recruitment of active Jil-1 kinase results in local chromatin decondensation (Deng et al, 2008). Hypomorphic mutations in Jil-1 and Chriz, by contrast, result in chromosome condensation and loss of interphase chromatin structure (Wang et al, 2001;Rath et al, 2006, Gan et al, 2011, similar to what is observed on loss of Z4 protein (Eggert et al, 2004). Besides these factors, we observed binding of the insulator proteins CP190 (Whitfield et al, 1988;Pai et al, 2004) and BEAF 32 (Zhao et al, 1995; data not shown) to the ectopic 61C7-8 position, resembling the situation at the endogenous site.…”

Section: Discussionmentioning

confidence: 99%

“…Phosphorylation of H3S10 by the histone kinase Jil-1 has been shown to contribute to local decondensation (Deng et al, 2008), and the Chriz-containing complex is involved in targeting Jil-1 to chromatin (Gan et al, 2011). Given that both Chriz and Jil-1 are found at the endogenous 61C7-8 interband ( Fig.…”

Section: Local Decondensation At the Transgenic Site Is Induced By Rementioning

confidence: 99%

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Dissection of open chromatin domain formation by site specific recombination inDrosophila

Zielke

Saumweber

2014

Journal of Cell Science

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Drosophila polytene interphase chromosomes provide an ideal test system to study the rules that define the structure of chromatin domains. We established a transgenic condensed chromatin domain cassette for the insertion of large pieces of DNA by sitespecific recombination. Insertion of this cassette into open chromatin generated a condensed domain, visible as an extra band on polytene chromosomes. Site-specific recombination of DNA sequence variants into this ectopic band allowed us to compare their capacity for open chromatin formation by cytogenetic methods. We demonstrate that the 61C7-8 interband DNA maintains its open chromatin conformation and epigenetic state at an ectopic position. By deletion analysis, we mapped the sequences essential for open chromatin formation to a 490-bp fragment in the proximal part of the 17-kb interband sequence. This fragment overlaps binding sites for the chromatin protein Chriz (also known as Chro), the histone kinase Jil-1 and the boundary element protein CP190. It also overlaps a promoter region that locates between the Rev1 and Med30 transcription units.

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The Chriz–Z4 complex recruits JIL-1 to polytene chromosomes,a requirement for interband-specific phosphorylation of H3S10

Cited by 31 publications

References 33 publications

Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains

Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains

Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation

Dissection of open chromatin domain formation by site specific recombination inDrosophila

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