The Chromatographic Purification and Ultra-Violet Spectrophotometric Estimation of Hydrastine and Berberine in Fluid Extract of Hydrastis

Ridi, M. S. El; Khalifa, K.; Mamoon, Amr

doi:10.1111/j.2042-7158.1956.tb12191.x

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Column chromatography followed by spectrophotometric determination of hydrastine and berberine in liquid extracts of hydrastis also was suggested (9). Berberine and coptisine in coptis rhizome were separated by constant-potential electrophoresis followed by fluorescence densitometry a t 550 nm (10).…”

mentioning

confidence: 99%

Colorimetric and spectrophotometric determinations of hydrastis alkaloids in pharmaceutical preparations

El-Masry

Korany

Abou‐Donia

1980

Journal of Pharmaceutical Sciences

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mentioning

confidence: 99%

Colorimetric and spectrophotometric determinations of hydrastis alkaloids in pharmaceutical preparations

El-Masry

Korany

Abou‐Donia

1980

Journal of Pharmaceutical Sciences

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“…A method using chromatography and ultraviolet spectrophotometry has been used for the assay of hydrastine and berberine in a fluid extract of hydrastis (163). Holubek and Volke (273) report the use of polarography in determining hydrastine and narcotine after hydrolysis with dilute hot nitric acid.…”

mentioning

confidence: 99%

Pharmaceuticals

Slack¹,

Mader²

1959

Anal. Chem.

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“…Goldenseal alkaloids have been isolated and their content evaluated using a variety of methods (Chadwick et al, 2001;Chen et al, 2000;El Ridi et al, 1956;Kim et al, 2004;Miyazaki et al, 1978;Schieffer & Pfeiffer, 2001), but the most typical method of analysis for the herb is via highperformance liquid chromatography (HPLC) (Abourashed & Khan, 2003;Betz et al, 1998;Chen & Chang, 1995;Leone et al, 1996;Li & Fitzloff, 2002;Schieffer & Kohn, 2002;U.S. Pharmacopeia, 2005;Weber et al, 2001Weber et al, , 2003a.…”

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confidence: 99%

Single-Laboratory Validation of a Method for the Detection and/or Quantification of Select Alkaloids in Goldenseal Supplements and Raw Materials by Reversed-Phase High-Performance Liquid Chromatography

Brown

Paley

Roman

et al. 2008

Pharmaceutical Biology

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Quality of botanical products and raw materials is important to manufacturers, regulators, researchers, and consumers. Many modern botanical quality-assurance schemes set specifications for select phytochemicals and measure against those specifications as one determinant of quality. While numerous publications describe procedures for determining compounds of interest in plant species, few methods have been systematically evaluated for accuracy, precision, or reliability, and often the analysis of finished products is not within the scope of the method. Hydrastis canadensis L., commonly referred to as Goldenseal, is an economically important North American medicinal plant that has been subject to adulteration in commerce. The phytochemicals of interest in the plant are the alkaloids hydrastine, berberine, and canadine. Of interest is also palmatine, an alkaloid found in potential adulterant species but not in goldenseal. In this study, goldenseal materials in raw, capsule, and tablet form, including an Echinacea/ Goldenseal combination product, were extracted with acidified water and acetonitrile and their hydrastine, berberine, canadine, and palmitine content determined by HPLC. The analytical method was optimized and evaluated in a single-laboratory validation study. Calibration curves for hydrastine and berberine were linear from 10 to 150 µg/mL. The limits of detection for palmatine and canadine were determined to be 0.5 µg/mL, which translates * Dedicated to Processor John Thor Arnason of the University of Ottawa, Department of Biology, on the occasion of his sixtieth birthday. to detection of levels of 0.004% w/w in test samples. Chromatographic resolution was achieved for all analytes in an isocratic 12.5-min chromatographic run employing a binary mobile phase. Triplicate determinations performed on 10 test materials by two analysts on 3 days resulted in relative standard deviations ranging from 0.9% to 3.4%.

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The Chromatographic Purification and Ultra-Violet Spectrophotometric Estimation of Hydrastine and Berberine in Fluid Extract of Hydrastis

Cited by 5 publications

References 4 publications

Colorimetric and spectrophotometric determinations of hydrastis alkaloids in pharmaceutical preparations

Colorimetric and spectrophotometric determinations of hydrastis alkaloids in pharmaceutical preparations

Pharmaceuticals

Single-Laboratory Validation of a Method for the Detection and/or Quantification of Select Alkaloids in Goldenseal Supplements and Raw Materials by Reversed-Phase High-Performance Liquid Chromatography

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