The Chronic and Unpredictable Stress Suppressed Kisspeptin Expression during Ovarian Cycle in Mice

Kim, Seung-Joon

doi:10.12750/jarb.34.1.40

Cited by 5 publications

(22 citation statements)

References 42 publications

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“…A total of 50–100 oocytes were lysed in RIPA buffer (GenDOPET, Texas, USA) containing protease inhibitors, and heated for 5 min at 98°C as previously described (Kim, 2019). Total oocyte proteins were separated on a 10–12% SDS-polyacrylamide gel.…”

Section: Methodsmentioning

confidence: 99%

EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte Meiotic Maturation in Mice

2021

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The cytoskeleton plays an orchestrating role in polarized cell growth. Microtubules (MTs) not only play critical roles in chromosome alignment and segregation but also control cell shape, division, and motility. A member of the plus-end tracking proteins, end-binding protein 1 (EB1), regulates MT dynamics and plays vital roles in maintaining spindle symmetry and chromosome alignment during mitosis. However, the role of EB1 in mouse oocyte meiosis remains unknown. Here, we examined the localization patterns and expression levels of EB1 at different stages. EB1 protein level was found to be stable during meiosis. EB1 mainly localized along the spindle and had a similar localization pattern as that of α-tubulin. The EB1 protein was degraded with a Trim-Away method, and the results were further confirmed with western blotting and immunofluorescence. At 12 h of culture after EB1 knockdown (KD), a reduced number of mature MII oocytes were observed. EB1 KD led to spindle disorganization, chromosome misalignment, and missegregation; β-catenin protein binds to actin via the adherens junctional complex, which was significantly reduced in the EB1 KD oocytes. Collectively, we propose that the impairment of EB1 function manipulates spindle formation, thereby promoting chromosomal loss, which is expected to fuel aneuploidy and possibly fertilization failure.

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Section: Methodsmentioning

confidence: 99%

EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte Meiotic Maturation in Mice

2021

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“…

Under the stressed condition, a complex feedback mechanism of the hypothalamo-pituitary-adrenal (HPA) axis is activated to regulate interactions to stress to maintain homeostasis of the body (Iwasa et al, 2018;Kim, 2019). In particular, the hormones in HPA axis with respect to corticotrophin releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and glucocorticoid reduce the pulsatile secretions of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH)

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confidence: 99%

“…36, No. 1, March 2021 adrenal (HPG) axis of animals, followed by impairments of ovarian cyclicity and synthesis/secretion of gonadotropin (FSH and LH) (Gai et al, 2016;Kim, 2019). Regarding the impairments by stress, several stressors in the rodent attenuate the ovarian cyclicity in regards to less number of secondary and antral follicles, and atresia induction in developing follicles (Wu et al, 2012;Kim, 2019).…”

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confidence: 99%

“…1, March 2021 adrenal (HPG) axis of animals, followed by impairments of ovarian cyclicity and synthesis/secretion of gonadotropin (FSH and LH) (Gai et al, 2016;Kim, 2019). Regarding the impairments by stress, several stressors in the rodent attenuate the ovarian cyclicity in regards to less number of secondary and antral follicles, and atresia induction in developing follicles (Wu et al, 2012;Kim, 2019). In case of alteration of reproduction hormone due to stress, because estradiol plays a key role in regulating the follicle development, ovarian atresia and growth of granulosa cells in the developing follicles, its decrease caused by stress is critical in the normal ovarian cyclicity and fate of granulosa cells (Li et al, 2016;Kim, 2019).…”

mentioning

confidence: 99%

“…Since the female exposed to several stressors is easy to be attenuated to their reproductive function in the hormone level (down regulations of gonadotropins and estrogen) and ovarian cyclicity, stressors may also change the expression pattern of CYP1A2 in the ovary Kim, 2019). However, the relationship between stress induction and CYP1A2 expression in the ovary is not understood well, in spite of the fact that the ovary functions can be affected by several factors (Hwang et al, 2020).…”

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confidence: 99%

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Immobilization stress increased cytochrome P450 1A2 (CYP1A2) expression in the ovary of rat

Hwang

Kim

Park

et al. 2021

J Anim Reprod Biotechnol

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Under the stressed condition, a complex feedback mechanism of the hypothalamo-pituitary-adrenal (HPA) axis is activated to regulate interactions to stress to maintain homeostasis of the body (Iwasa et al., 2018;Kim, 2019). In particular, the hormones in HPA axis with respect to corticotrophin releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and glucocorticoid reduce the pulsatile secretions of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH)

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Kisspeptin control of hypothalamus-pituitary-ovarian functions

Joy,

Chaube

2024

Vitamins and Hormones

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The Chronic and Unpredictable Stress Suppressed Kisspeptin Expression during Ovarian Cycle in Mice

Cited by 5 publications

References 42 publications

EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte Meiotic Maturation in Mice

EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte Meiotic Maturation in Mice

Immobilization stress increased cytochrome P450 1A2 (CYP1A2) expression in the ovary of rat

Kisspeptin control of hypothalamus-pituitary-ovarian functions

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