The Chronic Effect of Transgenic Maize Line with mCry1Ac or maroACC Gene on Ileal Microbiota Using a Hen Model

Chen, Liang; Zhong, Ruqing; Zhang, Hongfu

doi:10.3390/microorganisms7030092

Cited by 3 publications

(8 citation statements)

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“…The temperature was maintained at 22 ± 3 °C with a 16:8 h light/dark photocycle. All of the hens were kept healthy during the study period 25 …”

Section: Methodsmentioning

confidence: 99%

“…Raw fastq files were demultiplexed and quality‐filtered using QIIME, version 1.177 (http://qiime.org). 25 …”

Section: Methodsmentioning

confidence: 99%

“…All of the hens were kept healthy during the study period. 25 Organ sampling and histological analysis One bird per pen (eight birds per treatment) was humanely killed at the end of 12 weeks and a gross necropsy was performed. A complete gross visual pathology inspection of birds was conducted during the necropsy.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…Raw fastq files were demultiplexed and quality-filtered using QIIME, version 1.177 (http://qiime.org). 25 Operational taxonomic units were clustered with 97% similarity cut-off using USEARSH, version 7.1 (). The taxonomy of each 16S rRNA gene sequence was analyzed by RDP Classifier, version 2.2 (https://rdp.cme.msu.edu/classifier) against the Silva Release128 (http://www.arb-silva.de) 16S rRNA database using a confidence threshold of 70%.…”

Section: Illumina Miseq Sequencing and Bioinformatics Analysismentioning

confidence: 99%

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Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

et al. 2020

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BACKGROUND: The present study investigated the chronic effect on the composition and proportions of the cecal microbiota of laying hens for 12 weeks after consuming two genetically modified (GM) corns containing the maroACC gene from the Agrobacterium tumefaciens strain (CC) and the mCry1Ac gene from the Bacillus thuringiensis strain (BT) in comparison with the isogenic corn (CT). RESULTS: In total, 72 hens were randomly assigned to the CT corn-based diet, CC corn-based diet and BT corn-based diet. The absolute weights of abdominal fat, breast, thigh meat and organ weight were not affected by the dietary treatment. Highthroughput 16S rRNA gene sequencing revealed a few differences in the composition of cecal microbiota among the treatments. The only difference with respect to bacterial family was that the cecal abundance of Porphyromonadaceae (3.46 versus 2.11%; P = 0.073) tended to be higher for birds consuming the CC diet than those birds consuming the CT diet. Birds fed the BT diet tended to have a higher abundance of Barnesiella (0.62 versus 0.13%; P = 0.057) and a lower abundance of unclassified Ruminococcaceae (0.64 versus 1.19%; P = 0.097) than those fed the CT diet. Considering beneficial intestinal Barnesiella, this decreases and ultimately clears the colonization of vancomycin-resistant Enterococcus. The unclassified Ruminococcaceae was a low-frequency and low-abundance bacterial taxa and was not associated with intestinal pathology. CONCLUSION: These results indicate a similar modulation of cecal microbiota in laying hens by long-term feeding among transgenic CC corn, BT corn and non-transgenic corn and provide data for biosafety evaluation of the transgenic corn.

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“…The temperature was maintained at 22 ± 3 °C with a 16:8 h light/dark photocycle. All of the hens were kept healthy during the study period 25 …”

Section: Methodsmentioning

confidence: 99%

“…Raw fastq files were demultiplexed and quality‐filtered using QIIME, version 1.177 (http://qiime.org). 25 …”

Section: Methodsmentioning

confidence: 99%

Section: Animals and Experimental Designmentioning

confidence: 99%

Section: Illumina Miseq Sequencing and Bioinformatics Analysismentioning

confidence: 99%

See 2 more Smart Citations

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

et al. 2020

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“…Due to such rapid growth, GM crops are progressively becoming more and more part of consumers` diet whether directly or indirectly. Consumers are thus becoming more curious regarding the potential unintentional effects of these modern crops and their products on their health as well as the environment (Chen et al., 2019). For this purpose, different biosafety experiments have been conducted to assess the possible risks that could be associated with GM crops on non‐target organisms (NTOs).…”

Section: Introductionmentioning

confidence: 99%

Evaluation the effect of subchronic feeding of transgenic cotton line (CKC1) on the faecal microbiota of albino rabbits

Salisu

Shahid

Yaqoob

et al. 2020

Animal Physiology Nutrition

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Recent studies have demonstrated a strong relationship between the intestinal microbiota and the host health. As such, consumers are increasingly becoming more concerned about the potential effect of certain foods/feeds, particularly of transgenic origin on the gut microbiota. Although the European Food Safety Authority has recommended in their guidelines, to study the effect of transgenic food/feed on host‐microbiota, yet, few studies have focused on the evaluation of such effects mainly due to culturing difficulties. Therefore, this study was intended to evaluate the potential adverse effects of transgenic diet consumption on some specific gut microflora (Lactobacillus group, Bifidobacterium genus, Escherichia coli subgroup and Enterococcus genus) of rabbits. A total of forty‐eight rabbits were randomly assigned into four groups and fed a diet containing a variable proportion of transgenic cottonseeds at 0, 20, 30 and 40% inclusion level, respectively. Changes in the specific or total faecal bacterial population were monitored at five different experimental stages (i.e. 0, 45, 90, 135 and 180 days) using both the traditional plate count method (TM) and quantitative real‐time PCR (qPCR). No significant differences (p > .05) were observed concerning numbers of specific bacteria or total bacteria between the control and experimental groups, though qPCR showed numerically higher values in terms of 16S rRNA gene copies as compared to the values obtained from TM. However, such numerical differences were biologically insignificant (p > .05). Similarly, no significant variations were noticed in the calculated B/E (log10 copies of Bifidobacterium per g faces/log10 copies of E. coli genome per g faeces) ratios in all the groups. All the ratios were in the range of 1.24 to 1.30 throughout the experiment, indicating a good balance of intestinal microflora and greater resistance to intestinal disorders. It is therefore concluded that feeding transgenic cottonseeds could not adversely affect the gut microflora of rabbits during a long‐term study.

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Comparison of Chicken Cecal Microbiota after Metaphylactic Treatment or Following Administration of Feed Additives in a Broiler Farm with Enterococcal Spondylitis History

et al. 2021

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Minimizing the clinical signs of Enterococcus cecorum infections causing enterococcal spondylitis in broiler herds is successful when initiated as metaphylaxis in the first week of life. Mechanistically, either the Enterococcus species present at that time are reduced by antibiotic treatment or antibiotic treatment might induce changes in intestinal microbiota composition with an indirect and subsequent influence. The aim of the present study was to examine the cecal microbiota of chickens after administering lincospectin or different additives to evaluate whether these additives have lincospectin-like effects on microbiota. Therefore, 157,400 broiler chickens were reared in four chicken houses (~40,000 birds each) on a broiler farm with history of enterococcal spondylitis. Each flock was treated either with lincospectin or water soluble esterified butyrins, Bacillus (B.) licheniformis or palm oil was added via drinking water during the first days of life. Ten birds per house were dissected at days 11, 20 and 33 of life and cecal microbiota were analyzed (16S rRNA gene sequencing). Lincospectin treatment elicited significant changes in the cecal microbiota composition until slaughter age. Among the tested additives, effects of B. licheniformis on cecal microbiota composition were most similar to those seen after the treatment with lincospectin at day 11.

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The Chronic Effect of Transgenic Maize Line with mCry1Ac or maroACC Gene on Ileal Microbiota Using a Hen Model

Cited by 3 publications

References 42 publications

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

Evaluation the effect of subchronic feeding of transgenic cotton line (CKC1) on the faecal microbiota of albino rabbits

Comparison of Chicken Cecal Microbiota after Metaphylactic Treatment or Following Administration of Feed Additives in a Broiler Farm with Enterococcal Spondylitis History

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