The circadian clock has roles in mesenchymal stem cell fate decision

Gao, Wenzhen; Li, Rong; Meilin, Ye; Zhang, Lanxin; Zheng, Jiawen; Yang, Yuqing; Wei, Xiaoyu; Zhao, Qing

doi:10.1186/s13287-022-02878-0

Cited by 11 publications

(6 citation statements)

References 177 publications

(187 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, FCOPs did not elevate the expression of SIRT1 and even reduced its expression in HUVECs. SIRT1 is a NAD + -dependent coenzyme [49], and due to the significant decrease in cellular NAD + levels caused by hydrogen peroxide treatment, SIRT1 cannot be activated to function normally. This may explain the higher expression of SIRT1 protein in the H 2 O 2 control group.…”

Section: Discussionmentioning

confidence: 99%

The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria

Zhu,

Liu,

et al. 2024

Nutrients

View full text Add to dashboard Cite

Extensive in vivo investigations have demonstrated the antioxidant properties of fish collagen oligopeptides (FCOPs). One of the main causes of aging and chronic non-communicable diseases is oxidative stress. Therefore, FCOPs have a broad range of applications in illness prevention and delaying aging from the standpoint of the “food is medicine” theory. However, the mechanisms that underpin the antioxidant activity of FCOPs are not completely understood. The specific objective of this essay was to investigate the antioxidant effect of FCOPs and its possible mechanism at the cellular level. Mouse embryonic fibroblasts NIH/3T3 and human vein endothelial cells (HUVECs) were exposed to 200 µM hydrogen peroxide containing different concentrations of FCOPs for 4 h and were supplemented with different concentrations of FCOPs for 24 h. Normal growth medium without FCOPs was applied for control cells. An array of assays was used to evaluate the implications of FCOPs on cellular oxidative stress status, cellular homeostasis, inflammatory levels, and mitochondrial function. We found that FCOPs exerted a protective effect by inhibiting reactive oxygen species (ROS) production, enhancing superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS) activities and cell viability, inhibiting cell cycle arrest in the G1 phase, suppressing interleukin-1β (IL-1β), IL-6, matrix metalloproteinase-3 (MMP-3) and intercellular adhesion molecule-1(ICAM-1) secretion, downregulating nuclear factor-kappa B (NF-κB) activity, protecting mitochondrial membrane potential, and increasing ATP synthesis and NAD+ activities in both cells. FCOPs had a stronger antioxidant impact on NIH/3T3 than on HUVECs, simultaneously increasing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) content in NIH/3T3. These findings indicate that FCOPs have antioxidant effects on different tissue cells damaged by oxidative stress. FCOPs were therefore found to promote cellular homeostasis, inhibit inflammation, and protect mitochondria. Meanwhile, better health outcomes will be achieved by thoroughly investigating the effective dose and intervention time of FCOPs, as the absorption efficiency of FCOPs varies in different tissue cells.

show abstract

Section: Discussionmentioning

confidence: 99%

The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria

Zhu,

Liu,

et al. 2024

Nutrients

View full text Add to dashboard Cite

show abstract

“…Increasing evidence has suggested the critical role of the lysosome‐autophagy system in the cell's adaptive reaction to nanomaterials. [ 58,59 ] Nanomaterial‐induced autophagy may be determined by means of nanosize, physiochemical properties, and composites. [ 58,59 ] Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

“…[ 58,59 ] Nanomaterial‐induced autophagy may be determined by means of nanosize, physiochemical properties, and composites. [ 58,59 ] Zhang et al. compared Au nanoparticles with different diameters, among which 45 nm Au nanoparticles mostly promoted the level of autophagy and induced osteogenesis.…”

Section: Discussionmentioning

confidence: 99%

Enzymatically Bioactive Nucleus Pulposus Matrix Hydrogel Microspheres for Exogenous Stem Cells Therapy and Endogenous Repair Strategy to Achieve Disc Regeneration

Peng,

Chen,

Zhang

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Exogenous stem cell therapy and endogenous repair has shown great potential in intervertebral disc regeneration. However, limited nutrients and accumulation of lactate largely impair the survival and regenerative capacity of implanted stem cells and endogenous nucleus pulposus cells (NPCs). Herein, an injectable hydrogel microsphere (LMGDNPs) have been developed by immersing lactate oxidase (LOX)‐manganese dioxide (MnO2) nanozyme (LM) into glucose‐enriched decellularized nucleus pulposus hydrogel microspheres (GDNPs) through a microfluidic system. LMGDNPs showed a delayed release profile of LOX and satisfactory enzymatic capacity in consuming lactate. Mesenchymal stem cells (MSCs) plated on LMGDNPs exhibited better cell viability than cells on GelMA and decellularized nucleus pulposus microspheres (DNP) and showed a obviously increased NPCs phenotype. LMGDNPs prevented MSCs and NPCs death and promoted extracellular matrix synthesis by exhausting lactate. It is determined that LMGDNPs promoted NPCs autophagy by activating transforming growth factor β2 overlapping transcript 1 (TGFB2‐OT1), relying on the nanozyme. MSCs‐loaded LMGDNPs largely preserved disc hydration and alleviated matrix degradation in vivo. Summarily, LMGDNPs promoted cell survival and matrix regeneration by providing a nutrient supply, exhausting lactate, and activating autophagy via TGFB2‐OT1 and its downstream pathway and may serve as an ideal delivery system for exogenous stem cell therapy and endogenous repair.

show abstract

“…However, PER1 has also been shown to have diverse roles in the physiology of several stem cells, including embryonic, ADSC, MPCs, and dental pulp stem cells 42 . In mesenchymal progenitor populations, it has been shown to have significant effects on the differentiation of the cells, with osteoblastic, chondrogenic and adipogenic capacity affected by expression of PER1, or its downstream factors such as BMAL1 43 , with PER1/BMAL1 knockout animals having increased bone volume 44 .…”

Section: Discussionmentioning

confidence: 99%

Differential responses to aging amongst the transcriptome and proteome of mesenchymal progenitor populations

Duque,

Feehan,

Tripodi

et al. 2023

Preprint

View full text Add to dashboard Cite

The biological aging of mesenchymal stem cells is proposed to contribute to the development of a range of musculoskeletal and systemic diseases associated with older adults, such as osteoporosis, sarcopenia, and frailty. Despite this, little is understood about the specific mechanisms which drive this stem cell exhaustion, with most studies evaluating indirect effects of other aging changes, such as DNA damage, senescence, and inflammaging. In this study, we assess the transcriptomic and proteomic changes in three different populations of mesenchymal progenitor cells from older (50–70 years) and younger (20–40 years) individuals to uncover potential mechanisms driving stem cell exhaustion in mesenchymal tissues. To do this, we harvested primary bone marrow mesenchymal stem and progenitor cells (MPCs), circulating osteoprogenitors (COP), and adipose-derived stem cells (ADSCs) from younger and older donors, with an equal number of samples from males and females. These samples underwent RNA sequencing and label-free proteomic analysis, comparing the younger samples to the older ones. There was a distinct transcriptomic phenotype associated with the pooled older stem cells, indicative of suppressed proliferation and differentiation; however, there was no consistent change in the proteome of the cells. Older MPCs had a distinct phenotype in both the transcriptome and proteome, again consistent with altered differentiation and proliferation, but also a pro-inflammatory immune shift in older adults. COP cells showed a strong transcriptomic shift to pro-inflammatory signaling but no consistent proteomic phenotype. Similarly, ADSCs displayed transcriptomic shift in physiologies associated with cell migration, adherence, and immune activation, but no consistent proteomic change with age. These results show that there are underlying transcriptomic changes with stem cell aging that likely contribute to a decline in tissue regeneration; however, contextual factors such as the microenvironment and general health status also have a strong role in this.

show abstract

The circadian clock has roles in mesenchymal stem cell fate decision

Cited by 11 publications

References 177 publications

The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria

The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria

Enzymatically Bioactive Nucleus Pulposus Matrix Hydrogel Microspheres for Exogenous Stem Cells Therapy and Endogenous Repair Strategy to Achieve Disc Regeneration

Differential responses to aging amongst the transcriptome and proteome of mesenchymal progenitor populations

Contact Info

Product

Resources

About