The clinical impact of livestock-associated methicillin-resistant Staphylococcus aureus of the clonal complex 398 for humans

Becker, Karsten; Ballhausen, Britta; Kahl, Barbara C.; Köck, Robin

doi:10.1016/j.vetmic.2015.11.013

Cited by 77 publications

(80 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Livestock-associated MRSA. LA-MRSA can colonize humans and may go on to cause antimicrobial-resistant disease in some circumstances (Becker, Ballhausen, Kahl, & Köck, 2017;Soavi et al, 2010). Incidence of LA-MRSA human colonization appears variable, according to prevailing local livestock activity and MRSA colonization (Feingold et al, 2012).…”

Section: Current Issues For Amr In Livestock Bacteriamentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

124

View full text Add to dashboard Cite

Resistance to therapeutic antimicrobial agents is recognized as a growing problem for both human and veterinary medicine, and the need to address the issue in both of these linked domains is a current priority in public policy. Efforts to limit antimicrobial resistance (AMR) on farms have so far focused on control of the supply and use of antimicrobial drugs, plus husbandry measures to reduce infectious disease. In the United Kingdom and some other countries, substantial progress has been made recently against targets on agricultural antimicrobial drug use. However, evidence suggests that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite declining or zero antimicrobial drug use. Reasons for this are likely complex and varied but may include: bacterial adaptations to ameliorate fitness costs associated with maintenance and replication of resistance genes and associated proteins, horizontal transmission of genetic resistance determinants between bacteria, physical transfer of bacteria via movement (of animals, workers, and equipment), ineffective cleaning and disinfection, and co‐selection of resistance to certain drugs by use of other antimicrobials, heavy metals, or biocides. Areas of particular concern for public health include extended‐spectrum cephalosporinases and fluoroquinolone resistance among Enterobacteriaceae, livestock‐associated methicillin‐resistant Staphylococcus aureus, and the emergence of transmissible colistin resistance. Aspects of biosecurity have repeatedly been identified as risk factors for the presence of AMR on farm premises, but there are large gaps in our understanding of the most important risk factors and the most effective interventions. The present review aims to summarize the present state of knowledge in this area, from a European perspective.

show abstract

Section: Current Issues For Amr In Livestock Bacteriamentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

124

View full text Add to dashboard Cite

show abstract

“…First recognized in 2005 (Armand‐Lefevre et al ; Voss et al ), livestock‐associated methicillin‐resistant Staphylococcus aureus (LA‐MRSA) belonging to clonal complex (CC) 398 represents an occupational risk for those in close contact with livestock in many countries (Butaye et al ). In livestock‐dense areas, especially in mainland Europe, it can represent a significant proportion of human MRSA isolates (van Loo et al ; Larsen et al ; van Alen et al ) and may be responsible for a range of infections including serious illness (Becker et al ; Larsen et al ). Despite the recognition of CC398 LA‐MRSA in neighbouring mainland Europe its epidemiology has been less well‐studied in the United Kingdom.…”

Section: Introductionmentioning

confidence: 99%

Low prevalence of livestock‐associated methicillin‐resistantStaphylococcus aureusclonal complex 398 andmecCMRSA among human isolates in North‐West England

Paterson

2020

J Appl Microbiol

View full text Add to dashboard Cite

Aims Livestock‐associated methicillin‐resistant Staphylococcus aureus (LA‐MRSA) is an important public health problem in many countries. Despite reports of such isolates being found in both animals and humans in the United Kingdom few data are available on the prevalence in humans of such isolates. A prevalence study was therefore undertaken in the north‐west of England. Methods and Results One thousand two hundred and forty‐two human MRSA isolates collected during 2015 were screened by PCR to detect two of the major forms of LA‐MRSA: clonal complex (CC)398 and mecC MRSA. Isolates identified were further characterized by antimicrobial susceptibility testing and whole‐genome sequencing using HiSeq technology. A single mecC MRSA isolate and three CC398 LA‐MRSA were identified among the isolates screened. All four isolates were from MRSA screening. A phylogenetic analysis, including previously sequenced isolates from the United Kingdom, provided strong evidence for the genomic and epidemiological linkage between a pair of animal and human isolates of CC398 LA‐MRSA in England. These data are indicative of animal and humans isolates of CC398 LA‐MRSA being involved in the same transmission network and is the first demonstration of such closely linked animal and human isolates of this lineage in the UK. Conclusions The study indicates there is a low prevalence of CC398 LA‐MRSA and mecC MRSA among MRSA isolates in the sampled population. Significance and Impact of the Study While the study demonstrates that LA‐MRSA were rare among human MRSA isolates in the sampled population, the data provide a baseline for the future surveillance of what is a significant public health challenge in some regions. The demonstration of linked human and animal isolates of CC398 LA‐MRSA, supported by genomic and epidemiological data, reinforces the need for such surveillance and for continued awareness of the risks that LA‐MRSA may pose in the UK.

show abstract

“…Livestock-associated (LA-) CC398 strains may cause diseases in both humans and animals, e.g. wound infections and bacteremia 5 , foot joint infections in turkeys 6 and bovine mastitis 7, 8 . CC398 strains are often multiresistant to several classes of antimicrobial agents but they usually lack well-known virulence genes 9 , of which a significant number is located on mobile genetic elements, e.g.…”

Section: Introductionmentioning

confidence: 99%

Acquisition of virulence factors in livestock-associated MRSA: Lysogenic conversion of CC398 strains by virulence gene-containing phages

Kraushaar

Hammerl

Kienöl

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Staphylococcus aureus MRSA strains belonging to the clonal complex 398 (CC398) are highly prevalent in livestock and companion animals but may also cause serious infections in humans. CC398 strains in livestock usually do not possess well-known virulence factors that can be frequently found in other MRSA sequence types (ST). Since many staphylococcal virulence genes are residing on the genomes of temperate phages, the question arises why livestock-associated (LA-) CC398 strains are only rarely infected by those phages. We isolated and characterized four temperate phages (P240, P282, P630 and P1105) containing genes of the immune evasion cluster (IEC) and/or for the Panton-Valentine leucocidin (PVL). Sequence analysis of the phage genomes showed that they are closely related to known phages and that the DNA region encoding lysis proteins, virulence factors and the integrase exhibits numerous DNA repeats which may facilitate genomic rearrangements. All phages lysed and lysogenized LA-CC398 strains. Integration of IEC phage P282 was detected at ten sites of the hosts’ chromosome. The prophages were stably inherited in LA-CC398 and enterotoxin A, staphylokinase and PVL toxin were produced. The data demonstrate that lysogenic conversion of LA-CC398 strains by virulence-associated phages may occur and that new pathotypes may emerge by this mechanism.

show abstract

The clinical impact of livestock-associated methicillin-resistant Staphylococcus aureus of the clonal complex 398 for humans

Cited by 77 publications

References 76 publications

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Low prevalence of livestock‐associated methicillin‐resistantStaphylococcus aureusclonal complex 398 andmecCMRSA among human isolates in North‐West England

Acquisition of virulence factors in livestock-associated MRSA: Lysogenic conversion of CC398 strains by virulence gene-containing phages

Contact Info

Product

Resources

About