The Clinical Significance of miR-135b-5p and Its Role in the Proliferation and Apoptosis of Hippocampus Neurons in Children with Temporal Lobe Epilepsy

Li, Ruixiang; Hu, Jiahua; Cao, Sheng‐Li

doi:10.1159/000512949

Cited by 11 publications

(8 citation statements)

References 35 publications

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“…Chen et al [25] confirmed that miR-210 showed a high expression level in the hippocampus of epileptic animal models, while inhibition of miR-210 expression was able to inhibit seizure-induced apoptosis. Li et al [26] showed that miR-135b-5p expression levels were significantly lower in children with TLE, and overexpression of miR-135b-5p was able to target SIRT1 to reduce apoptosis after epilepsy. Hu et al [27] explored the expression profile of miRNAs in TLE rats, and miRNA microarray revealed 9 up-regulated miRNAs such as miR-146a and mR-210 and 15 down-regulated miRNAs such as miR-135b and miR-33.…”

Section: Discussionmentioning

confidence: 99%

MRI Features and Significance of Serum miRNAs and Inflammatory Cytokines in Patients with Temporal Lobe Epilepsy

Yao¹,

She²,

Song-lin³

et al. 2022

Concepts in Magnetic Resonance Part A

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This study was aimed to investigate the changes of brain MRI features and serum biological parameters in patients with TLE. 30 patients with unilateral TLE confirmed by surgical pathology were selected as study subjects, and 30 subjects without a history of epilepsy who underwent health examinations during the same period were selected as controls. The brain MRI features of the patients were explored and the T2 relaxation time (HCT2) indexes of the bilateral hippocampus were extracted. The differences in levels of peripheral blood T lymphocyte subsets, inflammatory cytokines, and miRNAs were measured. The results showed that the hippocampal volume of TLE patients was significantly reduced, and the HCT2 value of the hippocampus was greater than that of the control group ( P < 0.05 ). CD3+ (77.9 ± 4.4)%, CD4+ (45.6 ± 2.2)%, CD8+ (22.1 ± 1.9)%, and CD3+/CD8+ (2.24 ± 0.22) in peripheral blood T lymphocyte subsets of epileptic patients, compared with control group, CD3+, CD4+, and CD4+/CD8+ levels were significantly increased and CD8+ concentration was significantly decreased in epileptic patients ( P < 0.05 ); inflammatory cytokines TNF-α was (2.63 ± 0.26) pg/mL, IL-1β was (4.61 ± 0.57) pg/mL, IL-2 was (1.59 ± 0.21) pg/mL, IL-6 was (2.28 ± 0.19) pg/mL, and ICAM-1 was (1.89 ± 0.30) pg/mL in peripheral blood of epileptic patients, which was significantly increased compared with control group, while IL-10 was significantly decreased in epileptic patients ( P < 0.05 ); miR-146a was (2.14 ± 0.28) and miR-210 was (1.89 ± 0.31), miR-221 (2.44 ± 0.35), miR-34a (0.59 ± 0.14), miR-135b (10.17 ± 0.16), miR-33 (0.26 ± 0.09) in peripheral blood miRNA levels of epileptic patients, and miR-146a, miR-210, miR-221, and miR-34a levels of epileptic patients were significantly increased compared with control group, while MiR-135b and miR-33 levels of epileptic patients were significantly reduced ( P < 0.05 ). In summary, patients with TLE have hippocampal lesions, which may be related to peripheral blood T lymphocyte subsets imbalance, chronic inflammatory response, and abnormal expression of miRNAs.

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Section: Discussionmentioning

confidence: 99%

MRI Features and Significance of Serum miRNAs and Inflammatory Cytokines in Patients with Temporal Lobe Epilepsy

Yao¹,

She²,

Song-lin³

et al. 2022

Concepts in Magnetic Resonance Part A

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“…In our study, we found that miR-148a was downregulated in the DG region on days 14 and 60 post-SE. miR-135b-5p was shown to be downregulated in children with TLE and in an epileptic rat neuron model [ 36 , 37 ]. We found that miR-135b was upregulated on day 14 post-SE in the DG.…”

Section: Discussionmentioning

confidence: 99%

“…miR-146a and 142-3p are involved in the TLR signalling pathway [ 58 , 59 , 60 ]. miR-672 and 135b-5p are related to the pyroptosis pathway [ 36 , 37 , 61 ]. Among those, the PI3K-Akt-mTOR and MAPK signalling pathways were validated and changed the miRNAs-related pathway, which was consistent with our bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

Stage- and Subfield-Associated Hippocampal miRNA Expression Patterns after Pilocarpine-Induced Status Epilepticus

Dheen

Tang

et al. 2022

Biomedicines

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Objective: To investigate microRNA (miRNA) expression profiles before and after pilocarpine-induced status epilepticus (SE) in the cornu ammonis (CA) and dentated gyrus (DG) areas of the mouse hippocampus, and to predict the downstream proteins and related pathways based on bioinformatic analysis. Methods: An epileptic mouse model was established using a pilocarpine injection. Brain tissues from the CA and DG were collected separately for miRNA analysis. The miRNAs were extracted using a kit, and the expression profiles were generated using the SurePrint G3 Mouse miRNA microarray and validated. The intersecting genes of TargetScan and miRanda were selected to predict the target genes of each miRNA. For gene ontology (GO) studies, the parent-child-intersection (pci) method was used for enrichment analysis, and Benjamini-Hochberg was used for multiple test correction. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to detect disease-related pathways among the large list of miRNA-targeted genes. All analyses mentioned above were performed at the time points of control, days 3, 14, and 60 post-SE. Results: Control versus days 3, 14, and 60 post-SE: in the CA area, a total of 131 miRNAs were differentially expressed; 53, 49, and 26 miRNAs were upregulated and 54, 10, and 22 were downregulated, respectively. In the DG area, a total of 171 miRNAs were differentially expressed; furthermore, 36, 32, and 28 miRNAs were upregulated and 78, 58, and 44 were downregulated, respectively. Of these, 92 changed in both the CA and DG, 39 only in the CA, and 79 only in the DG area. The differentially expressed miRNAs target 11–1630 genes. Most of these proteins have multiple functions in epileptogenesis. There were 15 common pathways related to altered miRNAs: nine different pathways in the CA and seven in the DG area. Conclusions: Stage- and subfield-associated hippocampal miRNA expression patterns are closely related to epileptogenesis, although the detailed mechanisms need to be explored in the future.

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“…Neuroinflammation miR-146a [30,31] lncNEAT1 [69] miR-155 [32] lncMEG3 [70] lncH19 [75] lncUCA1 [76,77] Apoptosis/Neuronal loss miR-34a [33,34] lncH19 [74] circ_0003170 [94] miR-21 [35] lncMALAT1 [81] circ_UBQLN1 [95] miR-27a-3p [36] circ_DROSHA [99] miR-15a [37] miR-135b-5p [38] Synaptic plasticity miR-134 [41,42] lncBDNF-AS [79] miR-218 [43] lncMALAT1 [80] miR-132 [44]…”

Section: Pathaways Mirnas Lncrnas Circrnasmentioning

confidence: 99%

“…Another study showed that the expression of miR-135b-5p was significantly decreased in children with TLE and in the epileptic rat neuron model. The miR-135b-5p increased expression attenuates the postepileptic influence on cell viability and apoptosis by targeting SIRT1 [ 38 ]. All these data suggest the contribution of several miRNAs to the pathogenesis of epilepsy by affecting apoptosis, suggesting that modulation of these dysregulated miRNAs could provide novel strategies for the treatment of epilepsy.…”

Section: Role Of Mirnas In Tle Pathogenesismentioning

confidence: 99%

Non-Coding RNAs: New Biomarkers and Therapeutic Targets for Temporal Lobe Epilepsy

Manna

Fortunato

Benedittis

et al. 2022

IJMS

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Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy; it is considered a network disorder associated with structural changes. Incomplete knowledge of the pathological changes in TLE complicates a therapeutic approach; indeed, 30 to 50% of patients with TLE are refractory to drug treatment. Non-coding RNAs (ncRNAs), acting as epigenetic factors, participate in the regulation of the pathophysiological processes of epilepsy and are dysregulated during epileptogenesis. Abnormal expression of ncRNA is observed in patients with epilepsy and in animal models of epilepsy. Furthermore, ncRNAs could also be used as biomarkers for the diagnosis and prognosis of treatment response in epilepsy. In summary, ncRNAs can represent important mechanisms and targets for the modulation of brain excitability and can provide information on pathomechanisms, biomarkers and novel therapies for epilepsy. In this review, we summarize the latest research advances concerning mainly molecular mechanisms, regulated by ncRNA, such as synaptic plasticity, inflammation and apoptosis, already associated with the pathogenesis of TLE. Moreover, we discuss the role of ncRNAs, such as microRNAs, long non-coding RNAs and circular RNAs, in the pathophysiology of epilepsy, highlighting their use as potential biomarkers for future therapeutic approaches.

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The Clinical Significance of miR-135b-5p and Its Role in the Proliferation and Apoptosis of Hippocampus Neurons in Children with Temporal Lobe Epilepsy

Cited by 11 publications

References 35 publications

MRI Features and Significance of Serum miRNAs and Inflammatory Cytokines in Patients with Temporal Lobe Epilepsy

MRI Features and Significance of Serum miRNAs and Inflammatory Cytokines in Patients with Temporal Lobe Epilepsy

Stage- and Subfield-Associated Hippocampal miRNA Expression Patterns after Pilocarpine-Induced Status Epilepticus

Non-Coding RNAs: New Biomarkers and Therapeutic Targets for Temporal Lobe Epilepsy

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