The clinical significance of PINX1 expression in papillary thyroid carcinoma

Kang, JiHoon; Han, Kanghee; Kim, Hyeon Jin; Park, Ju Hui; Kong, Jun Suk; Park, Sunhoo; Myung, Jae Kyung

doi:10.1016/j.humpath.2018.07.004

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…Certain genes, such as PINX1, CCND2, and FGF2, have been demonstrated to be involved in PTC pathology by prior functional and experimental studies. [39][40][41] In summary, the potential molecular targets of PTC were preliminarily studied in this study. Firstly, RT 2 Profiler PCR Arrays were used to screen out targets that were similar to other tumors, and then the DEGs in PTC were selected through the mining and analysis of the TCGA database.…”

Section: Discussionmentioning

confidence: 99%

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Exploration of the Potential Biomarkers of Papillary Thyroid Cancer (PTC) Based on RT2 Profiler PCR Arrays and Bioinformatics Analysis

Peng¹,

Zhang²,

Cao³

et al. 2020

CMAR

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Background: Papillary thyroid carcinoma (PTC) has increased rapidly over recent years, and radiation, hormone effects, gene mutations, and others were viewed as closely related. However, the molecular mechanisms of PTC have not been cleared. Therefore, we intended to screen more accurate key genes and pathways of PTC by combining RT 2 profiler PCR arrays and bioinformatics methods in this study. Materials and Methods: RT 2 profiler PCR arrays were firstly analyzed to identify differential expression genes (DEGs) in PTC. RT-qPCR were performed to verify the most significant differential expression genes. The TCGA database was used to further verify for expanded data. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was analyzed. To construct the protein-protein interaction (PPI) network, we used STRING and Cytoscape to make module analysis of these DEGs. Results: Sixteen differentially expressed genes were presented in RT 2 profiler PCR arrays, including 13 down-regulated DEGs (DEGs) and three up-regulated DEGs (DEGs), while 13 stable DEGs were eventually verified. A total of 155 DEGs were presented in the TCGA database, including 82 up-regulated DEGs (DEGs) and 73 down-regulated DEGs (dDEGs). A total of 29 important genes were extracted after integrating these two results, GO and KEGG analyses were used to observe the possible mechanisms of action of these DEGs. The PPI network was constructed to observe hub genes. Prognostic analysis further demonstrated the involvement of these genes in the biological processes of PTC. Conclusion: This study identified some potential molecular targets and signal pathways, which might help us raise our awareness of the mechanisms of PTC.

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Section: Discussionmentioning

confidence: 99%

“…Certain genes, such as PINX1 , CCND2 , and FGF2 , have been demonstrated to be involved in PTC pathology by prior functional and experimental studies. 39 – 41 …”

Section: Discussionmentioning

confidence: 99%

Exploration of the Potential Biomarkers of Papillary Thyroid Cancer (PTC) Based on RT2 Profiler PCR Arrays and Bioinformatics Analysis

Peng¹,

Zhang²,

Cao³

et al. 2020

CMAR

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“…Immunohistochemistry. Immunohistochemical analysis was conducted by the automatic immunohistochemical staining equipment Lab vision Autostainer 480S (Lab Vision Corp., Fremont, CA, USA) according to the manufacturer's protocol based on our previous reports (27). The primary antibody used in this study was ZEB2 (1:200 dilution; polyclonal; Abcam, Cambridge, UK).…”

Section: Genementioning

confidence: 99%

The Role of ZEB2 Expression in Pediatric and Adult Glioblastomas

et al. 2021

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Background/Aim: Both pediatric glioblastoma (pGB) and adult glioblastoma (aGB) are clinically devastating, and are known to have different molecular pathogenesis. Here, we focused on the role of ZEB2 in pGB and aGB. Materials and Methods: Following transfection with ZEB2 siRNA into pGB cells (KNS42) and aGB cells (U87 and U373), cell proliferation, migration and invasion, and cell cycle progression were evaluated. Results: Targeted inhibition of ZEB2 induced up-regulation of E-cadherin expression and down-regulation of vimentin expression. Furthermore, it reduced invasion and migration of both pGB and aGB cells. Interestingly, in pGB cells, but not in aGB cells, silencing of ZEB2 reduced cell proliferation and viability, and affected the cell cycle progression of tumor cells. Conclusion: Inhibition of ZEB2 altered the mesenchymal features and reduced the migration and invasive ability of both pGB and aGB cells. ZEB2 effects were different in pGB and aGB cells regarding proliferation and cell cycle progression, suggesting that different underlying molecular mechanisms drive progression in these two types of tumors.

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“…In the past 20 years, its incidence has increased rapidly worldwide [ 3 ]. Although it is unclear whether the incidence increase reflects an increase in the true incidence or an improvement of diagnostic technology [ 4 , 5 ]. Papillary thyroid carcinoma (PTC) is the most common subtype of thyroid malignanncies, accounting for more than 80% of thyroid cancer, which originates from thyroid follicular epithelial cells [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

β-Catenin/LEF-1 transcription complex is responsible for the transcriptional activation of LINC01278

et al. 2021

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Background Our previous study shows that LINC01278 inhibits the malignant proliferation and invasion of papillary thyroid carcinoma (PTC) cells by regulating the miR-376c-3p/DNM3 axis. However, the regulation mechanism of LINC01278 expression in PTC cells is still unclear. Methods The luciferase reporter and ChIP assays were used to confirm the binding of LEF-1 to the putative promoter site of LINC01278 gene. The RNA immunoprecipitation and RNA pulldown were used to determine the enrichment of LINC01278 in β-catenin protein. The proteasome inhibitors (MG132) was used for detecting the β-catenin ubiquitination-proteasome degradation. Wnt/β-catenin specific agonists (LiCI), inhibitors (WiKI4) and TOP/FOP-flash reporter assay were used for detecting the activation of Wnt/β-catenin signal. Western blot was used to detected the expression of target proteins. Results The online PROMO algorithm determines a putative LEF-1 binding site on LINC01278 promoter, the LEF-1 binds to the putative promoter site of LINC01278 gene, and β-catenin enhances the binding of LEF-1 to the LINC01278 gene promoter. Furthermore, LINC01278 negatively regulated the protein accumulation of β-catenin in the cytoplasm, into nucleus, and ultimately inhibited the transcription of downstream target genes activated by Wnt/β-catenin signal. The results of RNA immunoprecipitation and RNA pulldown proved the direct binding of LINC01278 to β-catenin protein. In addition, the combination of LINC01278 and β-catenin promotes the β-catenin ubiquitination-proteasome degradation. Conclusion In summary, we found the transcriptional activation of LINC01278 by the β-catenin/LEF-1 transcription factor, and the negative feedback regulation of LINC01278 onβ-catenin signal.

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The clinical significance of PINX1 expression in papillary thyroid carcinoma

Cited by 5 publications

References 33 publications

<p>Exploration of the Potential Biomarkers of Papillary Thyroid Cancer (PTC) Based on RT<sup>2</sup> Profiler PCR Arrays and Bioinformatics Analysis</p>

<p>Exploration of the Potential Biomarkers of Papillary Thyroid Cancer (PTC) Based on RT<sup>2</sup> Profiler PCR Arrays and Bioinformatics Analysis</p>

The Role of ZEB2 Expression in Pediatric and Adult Glioblastomas

β-Catenin/LEF-1 transcription complex is responsible for the transcriptional activation of LINC01278

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