The Clinical Significance of the Histologic Subclassification of Colorectal Carcinoma

Teixeira, Cláudio Rolim; Tanaka, Shinji; Haruma, Ken; Yoshihara, Masaharu; Sumii, Koji; Kajiyama, Goro; Shimamoto, Fumio

doi:10.1159/000227235

Cited by 47 publications

(48 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TEIXEIRA et al (27,28) have demonstrated that moderately-poorly differentiated tumors present higher malignant potential, through the higher incidence of lymphatic and venous invasion and lymphonodal and hepatic metastasis. The data in the present study show a nonsignificant statistical difference in higher microvessel density of moderately-poorly differentiated tumors.…”

Section: Discussionmentioning

confidence: 99%

“…According to the histologic features at the deepest level of tumor invasion (the tumor apex), the tumors were classified as follows: well differentiated (W), moderately differentiated (M), and poorly differentiated (P). By assessing its glandular configuration and cellular arrangement, the M type was further subdivided in two different groups: moderately well differentiated (Mw) and moderately poorly differentiated (Mp) carcinomas (27) .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Tarta

Teixeira

Tanaka

et al. 2002

Arq. Gastroenterol.

Self Cite

View full text Add to dashboard Cite

-Background -Angiogenesis is a crucial step in tumor growth and progression. Its quantification by microvessel counting has a prognostic value in several types of malignancies and recently has been appraised in gastrointestinal tumors. Aim -To assess the prognostisc significance of microvessel quantification in colorectal carcinomas, studying its association with hematogenous metastases, survival and clinicopathological variables such as size, histologic differentiation and depth of tumoral invasion. Patients/Methods -Forty eight patients with colorectal adenocarcinoma were included in this study. Histologic sections of invasion tumoral margin (4 µm) were analyzed and endothellined microvessels were immunostained with monoclonal mouse Von Willebrand Factor (anti-FVIII). The microvessel count was performed from the identification of the area with increased microvessel density -hot spots -and results of the mean in five of these fields. Results -The cut-off microvessel count was 14 microvessels/0,785 mm 2 , which divided the sample into hypovascular and hypervascular groups. While 2/8 (25%) tumors with muscularis propria invasion were classified as hypervascular, 11/15 (73%) tumors with serosa or perivisceral fat were classified as hypervascular. However, a non-significant statistical association was found between the angiogenesis quantification, hematogenous metastases, survival and clinicopathological variables such as size and histologic differentiation of the tumor. Conclusions -The findings of significantly increase of microvessel count in conformity with tumoral invasion depth supports the hypothesis that tumor progression might be related to angiogenesis. Although angiogenesis is an important step in the tumoral growth and during the metastatization process, other factors can be implicated.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Tarta

Teixeira

Tanaka

et al. 2002

Arq. Gastroenterol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Microscopic examination of the main tumor was performed with hematoxylin and eosin staining. Histologic subclassification was performed as described previously [27, 28, 29, 30, 31, 39, 40]. In brief, well-differentiated (W), moderately differentiated (M), poorly differentiated (Por) and mucinous (Muc) adenocarcinomas were initially identified according to the WHO classification [41].…”

Section: Methodsmentioning

confidence: 99%

“…In brief, well-differentiated (W), moderately differentiated (M), poorly differentiated (Por) and mucinous (Muc) adenocarcinomas were initially identified according to the WHO classification [41]. We then subclassified the M lesions into moderately to well-differentiated (Mw; consisting of irregular glandular formations) and moderately to poorly differentiated (Mp; consisting of scattered single cells or islands of cells combined with small, irregular, glandular formations) adenocarcinomas [27, 28, 29, 30, 31, 39, 40]. Depth of invasion was classified into four groups according to the General Rules for the Study of Colorectal Cancer in Japan [42]: invasion of muscularis propria (mp); invasion of subserosa or subadventitia [ss (a1)]; invasion of serosa or adventitia [se (a2)], and invasion of adjacent structures [si (ai)].…”

Section: Methodsmentioning

confidence: 99%

Clinical Significance of Human Erythrocyte Glucose Transporter 1 Expression at the Deepest Invasive Site of Advanced Colorectal Carcinoma

Furudoi

Tanaka²,

Haruma³

et al. 2001

Oncology

Self Cite

View full text Add to dashboard Cite

Objective: Malignant cells exhibit increased glucose uptake and utilization in vitro and in vivo. This process is thought to be mediated by the glucose transporter (Glut) family. The aim of this study was to elucidate the clinical significance of Glut1 expression at the site of deepest invasion as a predictor of the invasive/metastatic potential and prognosis of advanced colorectal carcinoma (CRC). Methods: One hundred and fifty-two patients who had undergone surgical resection for advanced CRC were entered in this study. Histologic subclassifications at the deepest invasive site included well-differentiated (W), moderately to well-differentiated (Mw), moderately to poorly differentiated (Mp), poorly differentiated (Por) and mucinous (Muc) adenocarcinomas. Glut1 expression was examined immunohistochemically with a labeled streptavidin-biotin kit using anti-Glut1 polyclonal antibody MYM. As a marker of cell proliferation, Ki-67 expression was also examined. All immunoreactivity was analyzed at the deepest invasive site, central portion and superficial part. The immunohistochemical expression of Glut1 was defined as positive if distinct staining of the membrane or cytoplasm was observed in at least 30% of tumor cells. Results: Glut1 expression was detected in 56 of 152 lesions (36.8%) at the deepest invasive site. The incidence of Glut1 expression at the deepest invasive site correlated significantly with histologic grade (W/Mw grade, 28% vs. Mp/Por/Muc grade, 48%), depth of invasion (invasion of muscularis propria/invasion of subserosa or subadventitia, 29% vs. invasion of serosa or adventitia/invasion of adjacent structures, 52%), lymphatic invasion (absence of lymphatic invasion, 19% vs. presence of lymphatic invasion, 40%), lymph node metastasis (absence of lymph node metastasis, 25% vs. presence of lymph node metastasis, 41%) and Duke’s stage (Duke’s

show abstract

“…It is thought that carcinoma cells in the deepest sites of invasion have a greater capability to invade and metastasize than do carcinoma cells in other regions (38,39). Therefore, in 34 cases of pT 2 carcinoma, correlations of the immunohistochemical localization types of GalNAc-T3 at the deepest invading sites in the subserosal layer of pT 2 carcinoma, as a predictor of invasive/ metastatic potential, with the clinicopathological findings, mode of recurrence, and postsurgical survival were investigated.…”

Section: Introductionmentioning

confidence: 99%

Expression of UDP- N -Acetyl-α-d-Galactosamine-Polypeptide N -Acetylgalactosaminyltransferase Isozyme 3 in the Subserosal Layer Correlates with Postsurgical Survival of Pathological Tumor Stage 2 Carcinoma of the Gallbladder

Miyahara

Shoda²,

Kawamoto³

et al. 2004

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose and Experimental Design:Little is known about the molecular events leading to the development and progression of pathological tumor stage 2 (pT 2 ) gallbladder carcinoma. An alteration in the site of O-glycosylation may be associated with malignant behavior of carcinoma cells by modulation of the biological properties of the target mucin. The UDP-N-acetyl-␣-D-galactosamine-polypeptide N-acetylgalactosaminyltransferase isozyme 3 (GalNAc-T3) has the epithelial gland-specific expression and catalyzes mucintype O-glycosylation. In this study, immunohistochemistry was performed to determine the expression level of GalNAc-T3 in 34 cases of pT 2 gallbladder carcinoma to determine the correlation of the GalNAc-T3 expression level with mode of recurrence and postsurgical survival.Results: The expression levels of GalNAc-T3 protein and mRNA were increased in gallbladder carcinomas compared with the levels in adjacent noncancerous tissues and in intact gallbladders. Immunostaining of GalNAc-T3 was recognized in the cancerous epithelia, and the subcellular localization was classified into granular and diffuse types. In the 34 cases of pT 2 carcinoma, the localization of GalNAc-T3 was granular type in 50% and diffuse type in 50% of the cases at the deepest invading sites in the subserosal layer. Postsurgical recurrence was significantly more frequent in cases showing diffuse-type localization of GalNAc-T3 at the deepest invading sites (65%) than in those showing granular-type localization (23%; P < 0.05). Postsurgical survival was significantly poorer in cases showing diffusetype localization than in those showing granular-type localization (P ‫؍‬ 0.033) Conclusions: In pT 2 gallbladder carcinoma, the presence of diffuse-type localization of GalNAc-T3 in the subserosal layer is correlated with aggressiveness of the disease. This phenotype may serve as a unique biological feature associated with the malignant behavior.

show abstract

The Clinical Significance of the Histologic Subclassification of Colorectal Carcinoma

Cited by 47 publications

References 5 publications

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Clinical Significance of Human Erythrocyte Glucose Transporter 1 Expression at the Deepest Invasive Site of Advanced Colorectal Carcinoma

Expression of UDP- N -Acetyl-α-d-Galactosamine-Polypeptide N -Acetylgalactosaminyltransferase Isozyme 3 in the Subserosal Layer Correlates with Postsurgical Survival of Pathological Tumor Stage 2 Carcinoma of the Gallbladder

Contact Info

Product

Resources

About