The clinical utility of new reticulocyte and erythrocyte parameters on the Sysmex <scp>XN</scp> 9000 for iron deficiency in pregnant patients

Levy, S; Schapkaitz, Elise

doi:10.1111/ijlh.12904

Cited by 25 publications

(33 citation statements)

References 28 publications

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“…TSAT will have a lagging in declining during the early phase of iron deficiency . In addition, diurnal variations in TSAT and serum ferritin make these parameters unsuitable for screening tests …”

Section: Discussionmentioning

confidence: 99%

“…Serum ferritin was determined by a Cobas 6000 (Roche Diagnostics) automated analyser. All samples were analysed within 2 hours of collection because %Hypo‐He is influenced by sample stability …”

Section: Methodsmentioning

confidence: 99%

“…This parameter reflects iron status, specifically, Hb content <17 pg in the last 2‐3 months, and has been suggested to be a reliable indicator of iron deficiency, and %Hypo‐He is a suitable screening parameter because the measurement is rapid, has no incremental costs and does not require extra blood. Previous work has shown that %Hypo‐He is not influenced by blood donation, infection or inflammation, unlike biochemical tests …”

Section: Introductionmentioning

confidence: 96%

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Percentage of hypochromic red cells as a potential screening test to evaluate iron status in blood donors

Amir

Noor

Subbiah

et al. 2019

Int J Lab Hematology

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Introduction: Haemoglobin (Hb) levels are used to assess eligibility for blood donation but are not correlated with iron status. The percentage of hypochromic red cells (%Hypo-He) has been suggested as a useful screening parameter for iron deficiency.The aim of this study was to determine the cut-off level and accuracy of %Hypo-He screening among blood donors. Materials and Methods:A total of 170 blood donors were recruited into the study.Blood donors were classified into three groups: normal, latent iron deficiency and iron deficiency anaemia based on their Hb, serum ferritin and transferrin saturation (TSAT) levels. The diagnostic performance of %Hypo-He was evaluated with a validation group comprising 160 blood donors. Results:Receiver operating characteristic (ROC) curve analysis showed that %Hypo-He is an excellent parameter for detecting iron deficiency, with an area under the curve (AUC) of 0.906, a confidence interval (CI) of 0.854-0.957 at a cut-off of 0.6%, and 74.51% sensitivity and 88.24% specificity. A moderate negative correlation between %Hypo-He and TSAT (ρ = −0.576 [P < 0.001]) and a strong negative correlation between %Hypo-He and serum ferritin (ρ = −0.703 [P < 0.001]) were found. A cut-off value of 0.6% was applied to the validation group and showed 82.9% sensitivity and 96% specificity.Conclusion: %Hypo-He with a cut-off value of 0.6% is a potential parameter with high sensitivity and specificity for evaluating iron status among blood donors. This parameter is suitable for screening because its measurement has a faster turnaround time than biochemical markers. K E Y W O R D S blood donors, percentage of hypochromic red cells, screening parameter, serum ferritin, transferrin saturation How to cite this article: Amir N, Md Noor S, Subbiah I, Osman M, Seman Z. Percentage of hypochromic red cells as a potential screening test to evaluate iron status in blood donors. Int J Lab

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Percentage of hypochromic red cells as a potential screening test to evaluate iron status in blood donors

Amir

Noor

Subbiah

et al. 2019

Int J Lab Hematology

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“…In 2011, Mullier et al 6 described a two-step diagnosis tool using the percentage of microcytic erythrocytes (MicroR) (normal range: 0.3%-2.8%), the percentage of hypohaemoglobinized cells (%Hypo-He) (normal range: 0.1%-1.0%), the immature fraction of reticulocytes (IRF) (normal range: 1.6%-12.1%) and the reticulocyte count (normal range: 20 000-125 000/µL) to predict HS. 7 This algorithm is based on the physiopathology of HS exploiting the reticulocytosis with a decreased IRF level, and the microparticles production caused by weakened vertical linkages between the membrane skeleton and the lipid bilayer with its integral proteins. 6 Microcyte level has already been described as an excellent indicator of HS severity.…”

Section: Evaluation Of a Hereditary Spherocytosis Screening Algorithmmentioning

confidence: 99%

“…6 Microcyte level has already been described as an excellent indicator of HS severity. 8 This algorithm has been tested on the Sysmex XE-series [6][7][8][9] and was included in the guidelines for the laboratory diagnosis of hereditary red cell membrane disorders in 2015. 2 Here, we will share the results of our evaluation of the performance of this screening tool on the XN-series: the most recent generation of the Sysmex haematology analyser.…”

Section: Evaluation Of a Hereditary Spherocytosis Screening Algorithmmentioning

confidence: 99%

Evaluation of a hereditary spherocytosis screening algorithm by automated blood count using reticulocytes and erythrocytic parameters on the Sysmex XN‐series

Sottiaux

Favresse

Chevalier

et al. 2019

Int J Lab Hematology

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Diagnostic performance of reticulocyte hemoglobin equivalent in assessing the iron status

Chinudomwong

Binyasing

Trongsakul

et al. 2020

Clinical Laboratory Analysis

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Background: Measurement of reticulocyte hemoglobin equivalent (RET-He) is rapid, convenient, and cost-effective. Yet, researches on its performance in diagnosing iron deficiency with concurrent inflammation are limited. Hence, this study investigated RET-He value in various states, including inflammation, and evaluated its diagnostic performance in iron status assessment.Methods: Retrospectively, 953 clinical data and laboratory results-complete blood count, reticulocyte count, RET-He, and serum ferritin-were reviewed. Patients on iron therapy were excluded. Iron status was defined by serum ferritin as the reference method. RET-He among populations was investigated. Its diagnostic performance and optimal cutoff were determined by ROC analysis.Results: Three population groups were classified: healthy control, iron deficiency anemia (IDA), and non-ID anemia. Significantly, RET-He value in IDA was lower than that of healthy control, anemia of inflammation, and chronic kidney disease (P < .0001).Low RET-He was also observed in IDA with concomitant inflammation despite normal-to-high serum ferritin levels. No significant difference was observed between RET-He values in pure IDA and thalassemia (P = .57). ROC curve analysis revealed AUC of 0.876 (P < .0001) at cutoff 30 pg, by which IDA was discriminated with 74.2% sensitivity and 97.4% specificity. Applying cutoff ≤30 pg, IDA can be diagnosed with 96% sensitivity, 97.4% specificity, 80% PPV, and 99.6% NPV. Hence, RET-He >30 pg signifies a non-IDA state. Conclusion:In addition to convenience and cost-effectiveness, RET-He cutoff >30 pg can be potentially used to exclude IDA due to its excellent diagnostic sensitivity and specificity.

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The clinical utility of new reticulocyte and erythrocyte parameters on the Sysmex XN 9000 for iron deficiency in pregnant patients

Cited by 25 publications

References 28 publications

Percentage of hypochromic red cells as a potential screening test to evaluate iron status in blood donors

Percentage of hypochromic red cells as a potential screening test to evaluate iron status in blood donors

Evaluation of a hereditary spherocytosis screening algorithm by automated blood count using reticulocytes and erythrocytic parameters on the Sysmex XN‐series

Diagnostic performance of reticulocyte hemoglobin equivalent in assessing the iron status

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