The clinical value of lncRNA NEAT1 in digestive system malignancies: A comprehensive investigation based on 57 microarray and RNA-seq datasets

Abstract

This comprehensive investigation was performed to evaluate the expression level and potential clinical value of NEAT1 in digestive system malignancies. A total of 57 lncRNA datasets of microarray or RNA-seq and 5 publications were included. The pooled standard mean deviation (SMD) indicated that NEAT1 was down-regulated in esophageal carcinoma (ESCA, SMD = −0.35, 95% CI: −0.5~-0.20, P < 0.0001) and hepatocellular carcinoma (HCC, SMD = −0.47, 95% CI: −0.60~-0.34, P < 0.0001), while in pancreatic cancer (PC), NEAT1 was up-regulated (SMD = 0.45, 95% CI: 0.2~0.71, P = 0.001). However, NEAT1 expression in gastric cancer (GC), colorectal cancer (CRC), biliary tract cancer (BTC) and gallbladder carcinoma (GBC) showed no significant difference between cancer and control groups. The pooled area under the curve values for ESCA, GC, CRC, PC and HCC were 0.60, 0.89, 0.81, 0.77 and 0.69, respectively. Furthermore, our result demonstrated that a high expression of NEAT1 predicted an unfavorable prognosis in patients with digestive system malignancies (HR: 1.50, 95% CI: 1.28-1.76, P < 0.0001). Our study suggests that NEAT1 may play different roles in the initiation and progression of digestive system cancers and could be a potential diagnostic and prognostic biomarker in patients with digestive system carcinomas. Further and stricter studies with a larger number of cases are necessary to strengthen our conclusions.

Long noncoding RNA NEAT1‐modulated miR‐506 regulates gastric cancer development through targeting STAT3

“…27 LncRNA LINC00968 can induce NSCLC development by activating the Wnt pathway. 31 Here, in our study, we found that NEAT1 was significantly increased in GC cells compared with the normal gastric epithelial cells. 29 LncRNA TRERNA1 can promote GC progression by regulating EMT and enhancing SNAI1 expression.…”

Paraspeckles: Where Long Noncoding RNA Meets Phase Separation

“…Whilst many studies implicate the molecule NEAT1 in cancer progression, there are few studies directly connecting paraspeckles, or NEAT1_2, to cancer biology. A meta analysis of NEAT1 in cancer revealed a complex and varying pattern of expression revealing that NEAT1 can be either upregulated, downregulated, or unchanged in tumor compared to normal tissue, depending on different studies and cancer models, with most studies not differentiating between NEAT1 isoforms [50]. Despite this, the possibility for a role for paraspeckles in cancer is gaining more credence.…”

LncRNA NEAT1 Promotes Deterioration of Hepatocellular Carcinoma Based on In Vitro Experiments, Data Mining, and RT-qPCR Analysis

“…Our research group has previously performed a comprehensive analysis of the expression levels of NEAT1 in digestive system malignancies based on data from TCGA, GEO, ArrayExpress, and Oncomine [21]. The combined result based on 13 RNA-seq and microarray datasets showed that NEAT1 was downregulated in HCC.…”

“…Some investigators have demonstrated that NEAT1 plays essential roles in several malignancies, including breast cancer, lung cancer, laryngeal squamous cell cancer, gastric cancer, nasopharyngeal carcinoma, pancreatic carcinoma, and colorectal cancer [14][15][16][17][18][19][20]. Our research group previously conducted a comprehensive analysis of the clinical value of NEAT1 in digestive system malignancies based on 57 microarray and RNA-seq datasets, ultimately NEAT1 may play different roles in the initiation and progression of digestive system cancers [21]. The biological functions of NEAT1 in HCC have also been investigated in previous studies.…”