The Clinically Isolated FIZ15 Bacteriophage Causes Lysogenic Conversion in Pseudomonas aeruginosa PAO1

Vaca-Pacheco, Sergio; Paniagua-Contreras, Gloria Luz; García-González, Octavio Patricio; Garza, Mireya de la

doi:10.1007/pl00006794

Cited by 30 publications

(24 citation statements)

References 21 publications

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“…74,2010 ACCESSORY GENOME OF PSEUDOMONAS AERUGINOSA 629 on May 12, 2018 by guest http://mmbr.asm.org/ structure that both affects bacterial adhesion to eukaryotic cells and is an antigen recognized by host immune cells, seroconversion may potentially benefit the pathogenesis of P. aeruginosa infections by enhancing bacterial adherence or evasion of host immune responses. Indeed, PAO1 strains lysogenized with D3 display enhanced adherence to human buccal epithelial cells (217). Additionally, lysogeny with FIZ15, a phage that has similar properties to those of D3, is associated with increased resistance to phagocytosis by mouse peritoneal macrophages and increased resistance to killing by human serum (217).…”

Section: Prophages and Phage-like Elementsmentioning

confidence: 99%

“…Indeed, PAO1 strains lysogenized with D3 display enhanced adherence to human buccal epithelial cells (217). Additionally, lysogeny with FIZ15, a phage that has similar properties to those of D3, is associated with increased resistance to phagocytosis by mouse peritoneal macrophages and increased resistance to killing by human serum (217). In addition to modifying virulence, bacteriophages can also alter other aspects of P. aeruginosa biology.…”

Section: Prophages and Phage-like Elementsmentioning

confidence: 99%

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The Accessory Genome of Pseudomonas aeruginosa

Kung¹,

Ozer

Hauser

2010

Microbiol Mol Biol Rev

276

303

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SUMMARY Pseudomonas aeruginosa strains exhibit significant variability in pathogenicity and ecological flexibility. Such interstrain differences reflect the dynamic nature of the P. aeruginosa genome, which is composed of a relatively invariable “core genome” and a highly variable “accessory genome.” Here we review the major classes of genetic elements comprising the P. aeruginosa accessory genome and highlight emerging themes in the acquisition and functional importance of these elements. Although the precise phenotypes endowed by the majority of the P. aeruginosa accessory genome have yet to be determined, rapid progress is being made, and a clearer understanding of the role of the P. aeruginosa accessory genome in ecology and infection is emerging.

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Section: Prophages and Phage-like Elementsmentioning

confidence: 99%

Section: Prophages and Phage-like Elementsmentioning

confidence: 99%

The Accessory Genome of Pseudomonas aeruginosa

Kung¹,

Ozer

Hauser

2010

Microbiol Mol Biol Rev

276

303

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“…Lysogenic conversion likely supports phage survival indirectly by increasing the fitness of the host microbe, thus promoting the continued persistence of the phage genome within the host population. Examples of this phenomenon include the phage-mediated introduction of secreted virulence factors such as cholera toxin (30), altered lipopolysaccharide profile (35), and improved adhesion to epithelial cells (50).…”

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confidence: 99%

Interaction between Bacteriophage DMS3 and Host CRISPR Region Inhibits Group Behaviors ofPseudomonas aeruginosa

et al. 2009

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Bacteriophage infection has profound effects on bacterial biology. Clustered regular interspaced short palindromic repeats (CRISPRs) and cas (CRISPR-associated) genes are found in most archaea and many bacteria and have been reported to play a role in resistance to bacteriophage infection. We observed that lysogenic infection of Pseudomonas aeruginosa PA14 with bacteriophage DMS3 inhibits biofilm formation and swarming motility, both important bacterial group behaviors. This inhibition requires the CRISPR region in the host. Mutation or deletion of five of the six cas genes and one of the two CRISPRs in this region restored biofilm formation and swarming to DMS3 lysogenized strains. Our observations suggest a role for CRISPR regions in modifying the effects of lysogeny on P. aeruginosa.Bacteriophages are probably best known for their role as tools used to study bacteria. Phages have also served as important models for the study of mechanisms of transcription, recombination, and transposition (8). Bacteriophages also shape microbial populations both by impacting the size and structure of bacterial communities and through the transfer of genetic material between bacterial strains (6, 39, 52). It is estimated that phages can lyse as many as 20% of all bacterial cells daily; therefore, these infectious particles can have a profound impact on the evolution of microbes (53).While some bacteriophage infections are primarily lytic, temperate or lysogenic bacteriophages often integrate into the bacterial genome as a prophage causing a chronic infection of the host bacterium (52). In some cases, genes carried by a bacteriophage confer a new function upon a bacterium, typically not directly related to the phage life cycle, through a process known as lysogenic conversion. Lysogenic conversion likely supports phage survival indirectly by increasing the fitness of the host microbe, thus promoting the continued persistence of the phage genome within the host population. Examples of this phenomenon include the phage-mediated introduction of secreted virulence factors such as cholera toxin (30), altered lipopolysaccharide profile (35), and improved adhesion to epithelial cells (50).Here we report that infection of Pseudomonas aeruginosa PA14 by phage DMS3 results in lysogenized strains unable to form a biofilm or undergo swarming motility-two key group behaviors of this bacterium. Furthermore, we show that the loss of biofilm formation and swarming motility requires clustered regular interspaced short palindromic repeats (CRISPRs) and five of six cas (CRISPR-associated) genes. Our data suggest a complex interaction between microbe and bacteriophage impacts the group behaviors of P. aeruginosa. MATERIALS AND METHODSBacterial and phage culture preparation. Strains and plasmids used in this study are shown in Table 1. Overnight cultures were streaked from glycerol stocks stored at Ϫ80°C onto lysogeny broth (LB) agar (1.5%) and incubated overnight at 37°C to isolated single colonies. These colonies were then used to inoculate plankt...

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“…According to the Piggyback‐the‐Winner (PtW) model of marine holobionts, lysogeny for toxin production in mucus‐associated bacteria provides protection both to the bacteria (from phage superinfection and protist grazing) and to the underlying metazoan host, by blocking infections of bacterial pathogens and parasitic protists. This model also applies to analogous situations in human pathology, such as the survival of lysogenic Pseudomonas aeruginosa populations on mucosal surfaces in the lungs of cystic fibrosis patients …”

Section: The Virosphere As An Evolutionary R and D Sectormentioning

confidence: 99%

No genome is an island: toward a 21st century agenda for evolution

Shapiro

2019

Annals of the New York Academy of Sciences

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Conventional 20th century evolution thinking was based on the idea of isolated genomes for each species. Any possibility of life‐history inputs to the germ line was strictly excluded by Weismann's doctrine, and genome change was attributed to random copying errors. Today, we know that many life‐history events lead to rapid and nonrandom evolutionary change mediated by specific cellular functions. There are many ways that genomes, viruses, cells, and organisms interact to generate evolutionary variation. These include cell mergers and activation of natural genetic engineering by stress, infection, and interspecific hybridization. In addition, we know molecular mechanisms for transmitting life‐history information across generations through gametes. These discoveries require a new agenda for evolutionary theory and novel experimental designs to investigate the genomic impacts of stresses, biotic interactions, and sensory inputs coming from the environment. The review will offer some generic recommendations for enriching evolution experiments to incorporate new knowledge and find answers to previously excluded questions.

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The Clinically Isolated FIZ15 Bacteriophage Causes Lysogenic Conversion in Pseudomonas aeruginosa PAO1

Cited by 30 publications

References 21 publications

The Accessory Genome of Pseudomonas aeruginosa

The Accessory Genome of Pseudomonas aeruginosa

Interaction between Bacteriophage DMS3 and Host CRISPR Region Inhibits Group Behaviors ofPseudomonas aeruginosa

No genome is an island: toward a 21st century agenda for evolution

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