The clock that controls spine development modelled in a dish

Palla, Adelaida; Blau, Helen M.

doi:10.1038/d41586-020-00322-y

Cited by 3 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cells undergo species-characteristic oscillations similar to their in vivo counterparts. However, final stages of somite development and vertebra formation were not observed in currently published human cell culture systems ( Palla and Blau, 2020 ), suggesting that certain aspects of in vivo development are not recapitulated in these 2D systems. We reasoned that a 3D cell culture system may exhibit all the stages of PM development including morphogenetic processes associated with somite formation.…”

Section: Introductionmentioning

confidence: 96%

Paraxial mesoderm organoids model development of human somites

Budjan

Liu

Ranga

et al. 2022

eLife

View full text Add to dashboard Cite

During the development of the vertebrate embryo, segmented structures called somites are periodically formed from the presomitic mesoderm (PSM), and give rise to the vertebral column. While somite formation has been studied in several animal models, it is less clear how well this process is conserved in humans. Recent progress has made it possible to study aspects of human paraxial mesoderm development such as the human segmentation clock in vitro using human pluripotent stem cells (hPSCs), however, somite formation has not been observed in these monolayer cultures. Here, we describe the generation of human paraxial mesoderm (PM) organoids from hPSCs (termed Somitoids), which recapitulate the molecular, morphological and functional features of paraxial mesoderm development, including formation of somite-like structures in vitro. Using a quantitative image-based screen, we identify critical parameters such as initial cell number and signaling modulations that reproducibly yielded formation of somite-like structures in our organoid system. In addition, using single-cell RNA sequencing and 3D imaging, we show that PM organoids both transcriptionally and morphologically resemble their in vivo counterparts and can be differentiated into somite derivatives. Our organoid system is reproducible and scalable, allowing for the systematic and quantitative analysis of human spinal cord development and disease in vitro.

show abstract

Section: Introductionmentioning

confidence: 96%

Paraxial mesoderm organoids model development of human somites

Budjan

Liu

Ranga

et al. 2022

eLife

View full text Add to dashboard Cite

show abstract

Developmental genetics and recessive t-lethals

Erickson¹

2022

Twentieth Century Mouse Genetics

View full text Add to dashboard Cite

Paraxial mesoderm organoids model development of human somites

Budjan

Ranga

et al. 2021

Preprint

View full text Add to dashboard Cite

During the development of the vertebrate embryo, segmented structures called somites are periodically formed from the presomitic mesoderm (PSM), and give rise to the vertebral column. While somite formation has been studied in several animal models, it is less clear how well this process is conserved in humans. Recent progress has made it possible to study aspects of human paraxial mesoderm development such as the human segmentation clock in vitro using human pluripotent stem cells (hPSCs), however, somite formation has not been observed in these monolayer cultures. Here, we describe the generation of human paraxial mesoderm (PM) organoids from hPSCs (termed Somitoids), which recapitulate the molecular, morphological and functional features of paraxial mesoderm development, including formation of somite-like structures in vitro. Using a quantitative image-based screen, we identify critical parameters such as initial cell number and signaling modulations that reproducibly yielded somite formation in our organoid system. In addition, using single-cell RNA sequencing and 3D imaging, we show that PM organoids both transcriptionally and morphologically resemble their in vivo counterparts and can be differentiated into somite derivatives. Our organoid system is reproducible and scalable, allowing for the systematic and quantitative analysis of human spinal cord development and disease in vitro.

show abstract

The clock that controls spine development modelled in a dish

Cited by 3 publications

References 18 publications

Paraxial mesoderm organoids model development of human somites

Paraxial mesoderm organoids model development of human somites

Developmental genetics and recessive t-lethals

Paraxial mesoderm organoids model development of human somites

Contact Info

Product

Resources

About