The cloning and characterization of a maternally expressed novel zinc finger nuclear phosphoprotein (xnf7) in Xenopus laevis

Reddy, Bramham A.; Kloc, Małgorzata; Etkin, Laurence D.

doi:10.1016/0012-1606(91)90321-s

Cited by 100 publications

(58 citation statements)

References 25 publications

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“…Since the first TRIM was cloned from Xenopus laevis mRNA in 1991 [19], TRIM genes have been found in a wide range of species from fish [20] to pigs [21] to humans [22]. In total, genetic information is available for 68 human TRIMs (and several TRIM-like genes) [22], the majority of which have the characteristic really interesting new gene (RING)-finger, B-box and coiled coil (RBCC) domains (Figure 1 and Supplementary Figure 1).…”

Section: Structurementioning

confidence: 99%

Antiviral TRIMs: friend or foe in autoimmune and autoinflammatory disease?

2011

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Section: Structurementioning

confidence: 99%

Antiviral TRIMs: friend or foe in autoimmune and autoinflammatory disease?

2011

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“…Percent identity refers to just the RING finger domain. Alignment of B-box motifs: Sources for RING fingers and BBox motifs used in this comparison are RAD18 (Jones et al 1988); RPT-1 (Patarca et al 1988); PSC, SU(Z)2 (Brunk et al 1991); SS-A/Ro (Chan et al 1991); XNF7 (Reddy et al 1991); RAD16 (Bang et al 1992); PML (Kastner et al 1992); PAF-1 (Shimozawa et al 1992); PwA33 (Bellini et al 1993); MEL-18 (Ishida et al 1993); PAR-2 (Levitan et al 1994); BRCA1 (Miki et al 1994); HT2A (Fridell et al 1995); T18 (Miki et al 1991;LeDoyerin et al 1995); RFP (Cao et al 1997); RING1 (Satijn et al 1997); BMI-1 (Hemenway et al 1998); and XL43, XL75 (Perrin and Lacroix 1998).…”

Section: Tam-1 and Context-dependent Gene Silencingmentioning

confidence: 99%

The RING finger/B-Box factor TAM-1 and a retinoblastoma-like protein LIN-35 modulate context-dependent gene silencing in Caenorhabditis elegans

Hsieh¹,

Liu²,

Kostas³

et al. 1999

Genes & Development

103

105

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Context-dependent gene silencing is used by many organisms to stably modulate gene activity for large chromosomal regions. We have used tandem array transgenes as a model substrate in a screen for Caenorhabditis elegans mutants that affect context-dependent gene silencing in somatic tissues. This screen yielded multiple alleles of a previously uncharacterized gene, designated tam-1 (for tandem-array-modifier). Loss-of-function mutations in tam-1 led to a dramatic reduction in the activity of numerous highly repeated transgenes. These effects were apparently context dependent, as nonrepetitive transgenes retained activity in a tam-1 mutant background. In addition to the dramatic alterations in transgene activity, tam-1 mutants showed modest alterations in expression of a subset of endogenous cellular genes. These effects include genetic interactions that place tam-1 into a group called the class B synMuv genes (for a Synthetic Multivulva phenotype); this family plays a negative role in the regulation of RAS pathway activity in C. elegans. Loss-of-function mutants in other members of the class-B synMuv family, including lin-35, which encodes a protein similar to the tumor suppressor Rb, exhibit a hypersilencing in somatic transgenes similar to that of tam-1 mutants. Molecular analysis reveals that tam-1 encodes a broadly expressed nuclear protein with RING finger and B-box motifs.

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“…The 42 amino acid peptide corresponding to the B-Box motif (residues 219-260 in the XNF7 sequence; [25]) was synthesised on a Model 431A Applied Biosystems Solid Phase Synthesiser on Chydroxymethylphenoxycarbonyl resin using 9-fluorenylmethyloxy-carbonyl for temporary &-amino group protection [27]. Each amino acid was coupled twice as a hydroxybenzatriazole active ester automatically formed prior to use.…”

Section: Peptide Synthesis and Purificationmentioning

confidence: 99%

“…Consideration was also given to the known positions of the adjacent predicted a-helical coiled-coil domains and phosphorylation sites for XNF7 which exist between the RING finger domain and B-box [25,32]. This resulted in a domain comprising 42 amino acids bounded by predicted /?-turn regions adjacent to both the C-terminal a-helical coiled-coil domain and N-terminal phosphorylation sites, respectively.…”

Section: The B-box Domainmentioning

confidence: 99%

“…PML and T18 possess two B-box like domains which appear to form a subgrouping of the B-box family as highlighted by the presence of an extra potential cysteine metal ligand [16]. The other members of the family include RPT-I [23], SS-A/R0 [24], XNF7 [25] and PwA33 [26]. In order to understand the function of the B-box domain, both within this diverse family of proteins, and in relation to the RING finger and a-helical coiled-coil domains of the conserved tripartite motif, we have synthesised and purified a peptide corresponding to the B-box motif from the Xenopus protein XNF7.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of a novel cysteine/histidine‐rich metal binding domain from Xenopus nuclear factor XNF7

Borden¹,

Martin²,

O’Reilly

et al. 1993

FEBS Letters

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A 42 amino acid synthetic peptide corresponding to a newly defined cysteitmkistidine-rich protein motif called B-box, from the Xenopur protein XNF7 has been character&d. The metal-binding stokhiometry and dissociation constant for zinc were determined by competition with the chromopho~c chelator Br,BAPTA, demons~ating that one zinc atom binds per molecule of peptide despite the presence of seven putative metal ligands, and represents the t&t application of this method to measuring zinc stoichiometry of proteins and/or peptides. Cobalt binding studies indicate that the motif binds zinc more tightly than cobalt, that cysteines are used as ligands and that the cation is co-ordinated tetrahedrally. Circular dichroism and NMR studies both indicate that the B-box peptide is structured only in the presence of zinc, copper and to a lesser extent cobalt.

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The cloning and characterization of a maternally expressed novel zinc finger nuclear phosphoprotein (xnf7) in Xenopus laevis

Cited by 100 publications

References 25 publications

Antiviral TRIMs: friend or foe in autoimmune and autoinflammatory disease?

Antiviral TRIMs: friend or foe in autoimmune and autoinflammatory disease?

The RING finger/B-Box factor TAM-1 and a retinoblastoma-like protein LIN-35 modulate context-dependent gene silencing in Caenorhabditis elegans

Characterisation of a novel cysteine/histidine‐rich metal binding domain from Xenopus nuclear factor XNF7

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