The cloning, phylogenetic relationship and distribution pattern of two new putative GPCR-type octopamine receptors in the desert locust (Schistocerca gregaria)

Verlinden, Heleen; Vleugels, Rut; Marchal, Elisabeth; Badisco, Liesbeth; Tobback, Julie; Pflüger, Hans‐Joachim; Blenau, Wolfgang; Broeck, Jozef Vanden

doi:10.1016/j.jinsphys.2010.03.003

Cited by 38 publications

(41 citation statements)

References 32 publications

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“…Similar results were also found in DmOct2R, which is highly expressed in heads . However, in the desert locust S. gregaria, the transcript levels of SgOctR are the highest in the flight muscles, followed by the central nervous system (Verlinden et al, 2010b).…”

Section: Discussionmentioning

confidence: 98%

“…To date, only OctRs from Drosophila melanogaster (three subtypes) and Bombyx mori (two subtypes but only one have function) have been cloned and characterized (Chen et al, 2010;Maqueira et al, 2005). A partial sequence of putative OctR in the desert locust Schistocerca gregaria (SgOctR) was also cloned (Verlinden et al, 2010b). In the present study, we cloned a gene coding for a type 2 OctR (CsOA2B2) from the rice stem borer, Chilo suppressalis.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Yao

Huang

et al. 2012

Journal of Experimental Biology

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SUMMARYOctopamine, the invertebrate counterpart of adrenaline and noradrenaline, plays a key role in regulation of many physiological and behavioral processes in insects. It modulates these functions through binding to specific octopamine receptors, which are typical rhodopsin-like G-protein coupled receptors. A cDNA encoding a seven-transmembrane receptor was cloned from the nerve cord of the rice stem borer, Chilo suppressalis, viz. CsOA2B2, which shares high sequence similarity to CG6989, a Drosophila -adrenergic-like octopamine receptor (DmOct2R). We generated an HEK-293 cell line that stably expresses CsOA2B2 in order to examine the functional and pharmacological properties of this receptor. Activation of CsOA2B2 by octopamine increased the production of cAMP in a dose-dependent manner (EC 50 2.33nmoll Supplementary material available online at

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Yao

Huang

et al. 2012

Journal of Experimental Biology

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“…[18][19][20][21] As a peripheral neuromodulator, OCT modulates the activities of skeletal and visceral muscles, other peripheral target organs including fat body, oviduct, heart, and sensory organs, and gregarization in locusts. [22][23][24][25][26] As a centrally acting neuromodulator, OCT plays a major neuromodulatory role in regulating insect behaviors, such as rhythmic behaviors in locusts, 3,27 locomotion and grooming in fruit flies, 28 dance and sting behavior in honeybees, 29,30 sensitization and dishabituation of sensory input in locusts, 31,32 discrimination of nestmates from non-nestmates in honeybees and fire ants, 33,34 feeding behaviors of blowflies, cockroaches and honeybees, [35][36][37] division of labor and foraging preference in honeybees, 38,39 conditional courtship in fruit flies, 40 visual responses in locust and honeybees, 31,[41][42][43][44] learning and memory processes in honeybees, fruit flies, and crickets, [45][46][47][48][49] and many others (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…26,[131][132][133][134][135][136][137][138][139][140][141][142][143][144][145][146][147][148] Based on the structural and signaling similarities between cloned D. melanogaster octopaminergic receptors and vertebrate adrenergic receptors, Evans and Maqueira proposed a new classification. 149 According to this new classification ( Figure 4B), octopaminergic receptors were grouped into three classes, ie, α-adrenergic-like (OCTα-R), β-adrenergic-like (OCTβ-R), and octopaminergic/tyraminergic (OCT/TYR-R) or tyraminergic (TYR-R).…”

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confidence: 99%

“…150,151 Furthermore, the author has created a phylogenetic tree based on the comparison of 25 complete nucleotide sequences of insect octopaminergic and tyraminergic receptor genes by using the "Muscle" sequence alignment program. [153][154][155] Based on nucleotide sequence homology in the phylogenetic tree, insect octopaminergic receptor sequences from the moth M. sexta (MsOA), 146 D. melanogaster (OAMB) 137 and splice variants (DmOA1A, and DmOA1B), 143 A. mellifera (AmOA1), 138 P. americana (PaOA1), 142 B. mori (BmOAR1), 145 and locust Schistocerca gregaria (SgOctαR) 26 cluster together in the Octα-R class, whereas S. gregaria (SgOctβR), 26 B. mori (BmOAR2), 152 and D. melanogaster (DmOctβ1-R, DmOctβ2-R, DmOctβ3-R) 144 fall into the Octβ-R class ( Figure 5). Tyraminergic receptor sequences from A. mellifera (Amtyr1), 148 B. mori (BmTAR1), 140 (HvTA), 134 Agrotis ipsilon (Aityr/OCT), 141 Locusta migratoria (Lmtyr1), 139 and the cattle tick Boophilus microplus (BmiTA) 147 fall into the Tyr1-R subclass.…”

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Review of octopamine in insect nervous systems

Farooqui¹

2012

OAIP

148

155

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Octopamine (OCT) belongs to a group of compounds known as biogenic amines. OCT, a monohydroxylic analog of norepinephrine, is found in both vertebrate and invertebrate nervous systems. OCT is present in relatively high concentrations in the neuronal and nonneuronal tissues of most invertebrate species studied. However, OCT occurs as a trace amine in vertebrates where its physiological significance remains uncertain. OCT acts as a neurotransmitter, neuromodulator, and neurohormone in insect nervous systems where it prominently influences multiple physiological events. In the peripheral nervous system, OCT modulates the activity of flight muscles, peripheral organs, and most sense organs. In the central nervous system, OCT is essential for the regulation of motivation, desensitization of sensory inputs, arousal, initiation, and maintenance of various rhythmic behaviors, hygiene behavior, and complex social behaviors, including establishment of labor, as well as learning and memory. As a neurotransmitter, OCT regulates endocrine gland activity and controls the emission of light in the firefly lantern. As a neurohormone, OCT is released into hemolymph, transported to target tissues, and induces mobilization of lipids and carbohydrates, preparing insects for a period of extended activity or assisting recovery from a period of increased energy demand. OCT modulates hemocytic nodulation in nonimmune larvae and enhances phagocytosis as a neurohormone. OCT exerts its effects by binding to specific receptors belonging to the superfamily of G protein-coupled receptors and shares the structural motif of seven transmembrane domains. Activation of octopaminergic receptor types is coupled with different second messenger pathways depending on the species, tissue source, receptor type, and cell line used for expression of the cloned receptor. OCT-mediated generation of second messengers is associated with changes in cellular response, affecting insect behaviors. This review describes the roles of OCT in insect nervous systems at the behavioral and molecular levels.

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Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Huang

et al. 2019

Pest Management Science

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BACKGROUND Octopamine, the invertebrate counterpart of adrenaline and noradrenaline, regulates and modulates many physiological and behavioral processes in insects. It mediates its effects by binding to specific octopamine receptors, which belong to the superfamily of G‐protein coupled receptors (GPCRs). The expression profiles of octopamine receptor genes have been well documented in different developmental stages and multiple tissue types in several different insect orders. However, little work has addressed this issue in Hemiptera. RESULTS In this study, we cloned four octopamine receptor genes from brown planthopper. The deduced amino acid sequences share high identity with other insect homologues and have the characteristic GPCRs domain architecture: seven transmembrane domains. These genes were expressed in all developmental stages and examined tissues. The expression of NlOA2B3 and NlOA3 was relatively higher in egg and first instar nymph stage than in other stages and other receptor genes. All of these receptor genes were more highly expressed in brain than other tissues. CONCLUSION The identification of octopamine receptor genes in this study will provide a foundation for investigating the diverse roles played by NlOARs and for exploring specific target sites for chemicals that control agricultural pests. © 2019 Society of Chemical Industry

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The cloning, phylogenetic relationship and distribution pattern of two new putative GPCR-type octopamine receptors in the desert locust (Schistocerca gregaria)

Cited by 38 publications

References 32 publications

Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Review of octopamine in insect nervous systems

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

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