The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)

Kim, Nahui; Oh, Juyeol; Kim, Bichseam; Choi, Eung Kyu; Hwang, Un Sun; Staub, Jack E.; Chung, Sang-Min; Park, Young-Hoon

doi:10.1007/s13580-015-0024-2

Cited by 10 publications

(9 citation statements)

References 24 publications

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“…From a breeder perspective, monoecious plants are highly valuable because of the significant effort and time spent on isolation and emasculation (Kim et al., 2015); therefore, the newly formed monoecious types presented in this study (all F1 breeds with Tn‐92‐1026 as a father in addition to Miyaneh and Khatouni breeds with Tn‐92‐1026 as a mother plant) (Figure 3) might be an interesting material in breeding programmes, especially after few backcrosses and screening cycles to regain the temporarily lost parental economic traits and taking into consideration the maintenance of their monoecity.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis and inheritance of floral and fruit traits in melon (Cucumis melo) in the full diallel cross

et al. 2021

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To evaluate gene effects and heritability of fruit and flower characters in melon, three experiments were performed. In the first experiment, 48 genotypes of different melon groups were planted to choose the monoecious melon. To investigate the sex modification regarding fruit traits, seven parents, including a monoecious type and andromonoecious types, crossed in a full diallel scheme to obtain all possible combinations. Evaluation amongst all genotypes revealed one Cantaloupensis and all Flexuosus genotypes with monoecious flowers. Floral and fruit characters of F1 hybrids obtained from the diallel cross, were recorded. All hybrids that have been crossed with the monoecious parent revealed the monosexual progenies. Most of the floral characters with high GCA/SCA values remarked the additive gene effect. There were also cytoplasmic effects on flower and fruit traits. The significant GCA /SCA estimates observed for fruit traits such as yield, fruit length, fruit length to width, flesh thickness referred to the importance of additive gene effects. It could be demonstrated that classic hybridization holds great potential in controlling flowering habit and yield‐related fruit traits.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis and inheritance of floral and fruit traits in melon (Cucumis melo) in the full diallel cross

et al. 2021

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“…PCR을 기초로 하여 쉽고 빠르게 확인 가능한 마커 개발을 위해, CmACS-7 유전자의 3'-untranslation region(UTR) 지역의 Indel 부분에 AT 서열 반복수가 다른 것에 착안하여 공동우성 T1 마커에 대한 연구가 보고되었다 (Feng et al, 2009). 그러나 T1 마커를 이용한 PCR 분석에서 그 재현성이 낮았기 때문에, T1 마커에 염기를 추가하여 만들어진 sequence characterized amplified reg ion(SCAR) T1ex 마커가 멜론에서 새롭게 제시 되었다 (Kim et al, 2015). 이 마커를 이용하여 멜론의 단성화 라인 MO23과 양성화 라인 AM24 및 야생형 단성화 멜론, PI113112(Genbank accession no.…”

Section: 결과 및 고찰unclassified

“…이 마커를 이용하여 멜론의 단성화 라인 MO23과 양성화 라인 AM24 및 야생형 단성화 멜론, PI113112(Genbank accession no. EU791279.1)의 3-UTR 부분의 염기서열의 차이를 확인하였다 (Kim et al, 2015). 하지만…”

Section: 결과 및 고찰unclassified

“…2). 하지만 Kim et al(2015)에 따르면 야생형 멜론의 경우 단성화를 나타내는 약 190bp의 유전자형도 존재하는 것으 로 알려져( 높은 일치도를 바탕으로 참외에서 T1ex 마커의 MAS 적용 가능성을 평가하기 위하여 T1ex marker를 이용하여 MAS를 진…”

Section: 결과 및 고찰unclassified

“…최종 신장 단계로 72°C에서 5분간 반응 하였다. PCR 산물은 2.0% agarose gel에서 2시간동안 300volt로 분리시켰고, ethidium bromide를 이용하여 WiseDoc(Deahan, Seoul, Korea)의 디지털 카메라를 이용하여 UV 조건에서 PCR 결과물을 확 인하였다.멜론의 CmACS-7 유전자의 ORF-1 과 ORF-2 프라이머(Kim et al, 2015)를 이용하여 참외의 CmACS-7 유전자 염기서열…”

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Marker-Assisted Selection for Monoecy in Chamoe (Cucumis melo L.)

Bang¹,

Song²,

Sim³

et al. 2016

HST

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The DNA marker T1ex, originally developed from melon (Cucumis melo L.) for monoecy, was employed in chamoe, which is referred to as oriental melon. This marker shows size variations in monoecious melon. However, in chamoe, no such detrimental size variation was found in monoecious chamoe, and 99% association between flower phenotypes and genotypes of the T1ex marker was observed in 106 lines of chamoe. To evaluate the efficacy of the T1ex marker for marker-assisted selection (MAS), a total of 240 plants of chamoe breeding lines were screened using the T1ex marker. Among these, 98 varieties were selected. Although the T1ex marker might not be useful for MAS in melon, we found 100% concordance between genotypes and phenotypes for sex expression in chamoe. These results suggest that the T1ex marker will be a useful resource for MAS for monoecy in chamoe.

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Inheritance and gene mapping of spotted to non-spotted trait gene CmSp-1 in melon (Cucumis melo L. var. chinensis Pangalo)

Yan

et al. 2018

Mol Breeding

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The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)

Cited by 10 publications

References 24 publications

Genetic analysis and inheritance of floral and fruit traits in melon (Cucumis melo) in the full diallel cross

Genetic analysis and inheritance of floral and fruit traits in melon (Cucumis melo) in the full diallel cross

Marker-Assisted Selection for Monoecy in Chamoe (Cucumis melo L.)

Inheritance and gene mapping of spotted to non-spotted trait gene CmSp-1 in melon (Cucumis melo L. var. chinensis Pangalo)

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