The co-conversion of methane and mixtures of volatile fatty acids into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) expands the potential of an integrated biorefinery

Amabile, Claudia; Abate, Teresa; Chianese, Simeone; Musmarra, Dino; Muñoz, Raul

doi:10.1016/j.biortech.2023.129699

Cited by 15 publications

(1 citation statement)

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“…Both cosubstrates were supplied to represent 15% and 30% of the total carbon ( C tot ) present in the bottles, respectively. The fraction of carbon supplied through the cosubstrate was determined based on previous experimental studies . More specifically, it was demonstrated that a concentration of cosubstrate around 15% is sufficient for inducing 3-HV production, while 30% can be set as the upper tolerance limit for the biomass involved, at which the copolymer formation does not further increase.…”

Section: Methodsmentioning

confidence: 99%

Exploring New Strategies for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Methane and VFAs in Synthetic Cocultures and Mixed Methanotrophic Consortia

Amabile,

Abate,

Marcos

et al. 2024

ACS Sustainable Chem. Eng.

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In this work, the potential of a synthetic coculture and a mixed methanotrophic consortium to synthesize poly(3hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from renewable and waste-based feedstocks was assessed batchwise. Methylocystis parvuscocultivated with Rhodococcus opacus and a Methylocystisenriched culture previously grown on methane were subjected to nutrient starvation in a medium enriched with valeric acid (30% w w −1 of C tot ) or with a VFAs mixture containing acetic, propionic, butyric, and valeric acids (15% w w −1 of C tot ) under a CH 4 :O 2 or air atmosphere. For all test series, pH was adjusted to 7 after adding the cosubstrates, and a negligible substrate consumption or polymer production was considered the end point of the trial. Results showed that valeric acid promoted PHBV accumulation in both cultures regardless of the atmosphere. Interestingly, the mixture of VFAs supported PHBV accumulation only in the presence of methane. The highest PHBV contents for the coculture and the mixed consortium, equal to 73.7 ± 2.5% w w −1 and 49.6 ± 13% w w −1 , respectively, were obtained with methane and the VFAs mixture. This study demonstrates the suitability of cocultures and biobased cosubstrates for the sustainable production of the biodegradable polymer PHBV.

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Section: Methodsmentioning

confidence: 99%