The co-expression of GPER and Gankyrin in ovarian endometriosis and its correlation with the rASRM stages

Zhang, Chun; Yuan, Xiying; Zhang, Yi

doi:10.1007/s00404-015-3807-x

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…Researches have proven the pathological roles of GPER in a diverse array of disorders and GPER is emerging as a novel therapeutic target and prognostic indicator (24). In endometriosis, GPER expression in EuEM has been demonstrated to be relatively higher than in CoEM (25–27). However, there is no report to explore its follow-up effects after activation by E 2 or other ligands.…”

Section: Introductionmentioning

confidence: 99%

Estrogen stabilizes hypoxia-inducible factor 1α through G protein-coupled estrogen receptor 1 in eutopic endometrium of endometriosis

Zhang

Xiong

et al. 2017

Fertility and Sterility

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Objective To investigate whether G protein-coupled estrogen receptor (GPER, also known as GPR30 and GPER1) stabilizes Hypoxia inducible factor 1α (HIF-1α) in eutopic endometrium (EuEM) of endometriosis? Design Immunohistochemical analysis and experimental in vitro study. Setting University hospital Patient(s) Patients with or without endometriosis Intervention(s) The EuEM and normal control endometrium (CoEM) were obtained by curettage. Primary cultured endometrial stromal cells (ESCs) were treated with 17β-estrogen (E2), G1 or G15. Main Outcome Measure(s) The EuEM and CoEM were collected for immunohistochemistry. Western blot, PCR, Elisa, and dual luciferase experiments were used to detect expression of GPER, HIF-1α, VEGF, and MMP9 in ESCs. E2 and G1 were used as agonists of GPER while G15 as an antagonist. Migration of ESCs and endothelial tube formation of HUVECs cultured in medium collected from ESCs were measured. Results Protein levels of GPER and HIF-1α were higher in EuEM than in CoEM. HIF-1α protein levels but not HIF-1α mRNA levels increased concurrently with GPER after E2 and G1 treatment. Furthermore, expression and activity of VEGF and MMP9 increased under E2 and G1 stimulation. However these effects disappeared when GPER was blocked. Conclusion GPER stabilizes HIF-1α thus promotes HIF-1α induced vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP9) in ESCs, which plays critical roles in endometriosis.

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Section: Introductionmentioning

confidence: 99%

Estrogen stabilizes hypoxia-inducible factor 1α through G protein-coupled estrogen receptor 1 in eutopic endometrium of endometriosis

Zhang

Xiong

et al. 2017

Fertility and Sterility

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“…Regarding hormonal influence, it was clearly demonstrated that blood vessel formation within endometriotic lesions is stimulated by estrogens, 29,30 while progesterone inhibits it. 31 In the present study, we failed to demonstrate any influence of hormonal therapy on NIR-ICG vascular pattern, because, according to our clinical practice, we enrolled all women taking progestinic therapy in order to reduce pain symptoms’ severity before surgery.…”

Section: Discussionmentioning

confidence: 99%

Rectosigmoid Endometriosis Vascular Patterns at Intraoperative Indocyanine Green Angiography and their Correlation with Clinicopathological Data

et al. 2020

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Introduction. The objective of the study was to evaluate the presence of different rectosigmoid endometriosis (RSE) vascular patterns using intraoperative indocyanine green (ICG) angiography and their correlation with clinicopathological data. Material and Methods. A prospective pilot study on 30 consecutive symptomatic women affected by RSE and scheduled for minimally invasive surgery between May 2018 and January 2019. ICG was used for the intraoperative evaluation of RSE vascularization. Perfusion grade was classified as follows: 0-1 = no or low fluorescence (hypovascular pattern); 2 = regular fluorescence, similar to healthy surrounding rectosigmoid tract (isovascular pattern); and 3-4 = diffuse or abundant fluorescence (hypervascular pattern). Results. Thirty women were intravenously injected with ICG after nodule exposure. No adverse effects related to ICG use were noted. After a 5- to 50-s latency from ICG injection, the real-time direct visualization of RSE perfusion showed diffuse or abundant fluorescence in 12/30 (40%) women, while in the remaining 18/30 (60%), fluorescence was poor or absent. No statistical differences were observed between the 2 groups regarding preoperative, intraoperative, and histological variables analyzed, except for a maximum diameter of bowel lesions and microvessel density (MVD). Hypovascular nodules had a larger maximum diameter (39.5 ± 15.6 mm vs 30.3 ± 11.4 mm, P < .05) and lower MVD (154.6+/43.6 vs 281.1+/−77.4, P < .05) than hypervascular ones. Conclusions. ICG angiography is a feasible and safe technique to intraoperatively assess RSE vascularization. The majority (60%) of endometriotic nodule presented a hypovascular pattern. The hypovascular pattern seems to be associated with a larger nodule size and lower MVD.

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“…Although surgery is effective in treating endometriosis in adults, it may well be a double-edged sword in adolescents. Aside from the increase in risk of premature ovarian failure caused by surgical treatment of ovarian endometriomas (Seyhan et al, 2015), recent animal and epidemiological studies indicate that surgery, in and by itself, may promote the development of endometriosis (Liu et al, 2016;Long et al, 2016;Zhang et al, 2016aZhang et al, , 2016c. In fact, a history of surgery for endometriosis is correlated with the presence and severity of deep infiltrating endometriosis, underlining the necessity of a thorough preoperative assessment and the need for providing complete information to these patients before undertaking further surgery (Sibiude et al, 2014).…”

Section: Medical Treatmentmentioning

confidence: 99%

“…In principle, the same drugs can be used in adolescent and adult patients. The critical issue, however, is the mentioned progressive and dynamic nature of endometriosis, shown both in spontaneous and induced disease (D'Hooghe et al, 1996;Harirchian et al, 2012;Zhang et al, 2016aZhang et al, , 2016bZhang et al, , 2016c.…”

Section: Medical Treatmentmentioning

confidence: 99%

Progress in the diagnosis and management of adolescent endometriosis: an opinion

Benagiano

Guo

Puttemans

et al. 2018

Reproductive BioMedicine Online

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Increasing evidence indicates that early onset endometriosis (EOE), starting around menarche or early adolescence, may have an origin different from the adult variant, originating from neonatal uterine bleeding (NUB). This implies seeding of naïve endometrial progenitor cells into the pelvic cavity with NUB; these can then activate around thelarche. It has its own pathophysiology, symptomatology and risk factors, warranting critical management re-evaluation. It can also be progressive, endangering future reproductive capacity. This variant seems to be characterized by the presence of ovarian endometrioma. Today, the diagnosis of endometriosis in young patients is often delayed for years; if rapidly progressive, it can severely affect pelvic organs, even in the absence of serious symptoms. Given the predicament, great attention must be paid to symptomatology that is often non-specific, justifying a search for new, simple, non-invasive markers of increased risk. Better use of modern imaging techniques will aid considerably in screening for the presence of EOE. Traditional laparoscopy should be limited to cases in which imaging gives rise to suspicion of severity and a stepwise, minimally invasive approach should be used, followed by medical treatment to prevent recurrence. In conclusion, EOE represents a condition necessitating early diagnosis and stepwise management, including medical treatment.

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The co-expression of GPER and Gankyrin in ovarian endometriosis and its correlation with the rASRM stages

Abstract: GPER and Gankyrin might be implicated in the hormonal regulation of endometriosis and be associated with the severity of endometriosis. In addition, GPER and Gankyrin were found to be positively correlated, which could possibly serve as novel therapeutic targets for this disease.

Cited by 10 publications

References 26 publications

Estrogen stabilizes hypoxia-inducible factor 1α through G protein-coupled estrogen receptor 1 in eutopic endometrium of endometriosis

Estrogen stabilizes hypoxia-inducible factor 1α through G protein-coupled estrogen receptor 1 in eutopic endometrium of endometriosis

Rectosigmoid Endometriosis Vascular Patterns at Intraoperative Indocyanine Green Angiography and their Correlation with Clinicopathological Data

Progress in the diagnosis and management of adolescent endometriosis: an opinion

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