The Coagulant Action of Crystalline Trypsin, Cephalin and Lung Extracts

Ferguson, John H.; Erickson, Betty Nims

doi:10.1152/ajplegacy.1939.126.3.661

Cited by 46 publications

(13 citation statements)

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“…Trypsin activation (21,22). To 1 ml of a 125 pg/ml solution of normal or patient II-7 "step 3" prothrombin were added 0.1 ml of 0.1 M CaCi, and 0.1 ml of a 50 pug/ml solution of trypsin in 0.001 M HCU.…”

Section: Prothrombin Activationmentioning

confidence: 99%

Congenital dysprothrombinemia: an inherited structural disorder of human prothrombin

Shapiro¹,

Martinez²,

Holburn³

1969

J. Clin. Invest.

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Section: Prothrombin Activationmentioning

confidence: 99%

Congenital dysprothrombinemia: an inherited structural disorder of human prothrombin

Shapiro¹,

Martinez²,

Holburn³

1969

J. Clin. Invest.

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“…There have been reports that intravenous trypsin caused shock (16), intravascular coagulation (17,19) local necrosis (18), sloughing of tissue (18), and was generally "too toxic for intravenous use" (19). Occasionally, shock did appear when doses exceeding 150,000 units of trypsin were given to rabbits, or greater than 750,000 units were given to dogs.…”

Section: ) Experimental Production Of Thrombi In Rabbits Andmentioning

confidence: 99%

Intravenous Trypsin: Its Anticoagulant, Fibrinolytic and Thrombolytic Effects 1

Innerfield¹,

Schwarz²,

Angrist³

1952

J. Clin. Invest.

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Fibrinogenolysis and prolonged blood coagulation have been observed following large intravenous doses of crystalline trypsin ( 1 ) .2 The present study was undertaken to evaluate further the effects of intravenous crystalline trypsin upon the protein components of the coagulation mechanism and the fibrin content of artificially induced intravascular thrombi, in 60 rabbits and 25 dogs. A striking observation in this study was the lack of untoward reactions, including shock, when the rate and concentration of injected trypsin were kept within carefully calculated limits. MATERIALS 1) Sixty rabbits whose average weight was 2 kg. were fed rabbit food pellets and given water ad libitum.2 Quick (2). Fibrinogen was determined by the method of Greenberg (3). The Innerfield, Angrist, and Benjamin method (4) of antithrombin titer determination was used. Factor V was measured by the method of Stefanini (5); and fibrinolysis assay was made by the Conley, Ratnoff, and Hartmann method (6). Determinations of coagulation time were made by the Lee-White method (7). Blood volume was estimated following administration of labeled albumin (8). Continuous measurements of clotting viscosities were made with a RichRoth Ultra-viscosimeter (9). PROCEDURE 1) Rabbits. Blood samples were obtained from non-anesthetized rabbits by cardiac puncture, before and at varying intervals during infusion of trypsin. Sections of an ear vein thrombus were obtained for histologic study prior to, during, and after completion of each intravenous infusion.2) Dogs. Trypsin infusions were given to dogs who had been anesthetized with 6 per cent Nembutal. Blood samples were obtained prior to, and during the course of the infusion. In securing 1049

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“…The coagulant action of trypsin is more indirect, and they concluded that it activates prothrombin, thus appearing to behave like "thrombokinase," which, they suggested, might itself be a proteolytic enzyme. Ferguson and Erickson (73) showed that the action of trypsin on prothrombin is potentiated by "cephalin" and calcium ions. A similar analogy was provided by the venom of the Russell's viper which also required both calcium ions and phospholipid for its immensely powerful coagulant action on plasma (153,156,260).…”

Section: Introductionmentioning

confidence: 99%