The codon CUG is read as serine in an asporogenic yeast Candida cylindracea

Kawaguchi, Yoshiyuki; Honda, Hiroshi; Taniguchi-Morimura, Junko; Iwasaki, Shinsuke

doi:10.1038/341164a0

Cited by 222 publications

(122 citation statements)

References 18 publications

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“…Similar observations were previously made in several Candida species [13,14] and Debaryomyces hansenii [15]. Unfortunately, we have not been able to identify the corresponding tRNA gene suggesting that it is present at a low copy number.…”

Section: Trnassupporting

confidence: 86%

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila

et al. 2000

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Section: Trnassupporting

confidence: 86%

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila

et al. 2000

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“…Assuming that a similar signalling cascade might exist in C. albicans and that the corresponding homologous genes could be expressed in S. cerevisiae despite the deviation from universal translation rules for some Candida species (32,60,69), we were able to isolate a complementing C. albicans homolog of the S. cerevisiae SLT2 gene, suggesting that a signalling cascade homologous to the Pkc1-controlled cascade of S. cerevisiae is functional in this opportunistic fungus. The isolated gene, named MKC1, replaced the function of SLT2 in S. cerevisiae null mutants by restoring growth and preventing autolysis at 37ЊC, thus showing that the Candida gene is expressed in Saccharomyces cells.…”

Section: Discussionmentioning

confidence: 79%

Functional Characterization of the MKC1 Gene of Candida albicans, Which Encodes a Mitogen-Activated Protein Kinase Homolog Related to Cell Integrity

Navarro‐García

Sánchez

Pla

et al. 1995

Molecular and Cellular Biology

182

154

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Mitogen-activated protein (MAP) kinases represent a group of serine/threonine protein kinases playing a central role in signal transduction processes in eukaryotic cells. Using a strategy based on the complementation of the thermosensitive autolytic phenotype of slt2 null mutants, we have isolated a Candida albicans homolog of Saccharomyces cerevisiae MAP kinase gene SLT2 (MPK1), which is involved in the recently outlined PKC1-controlled signalling pathway. The isolated gene, named MKC1 (MAP kinase from C. albicans), coded for a putative protein, Mkc1p, of 58,320 Da that displayed all the characteristic domains of MAP kinases and was 55% identical to S. cerevisiae Slt2p (Mpk1p). The MKC1 gene was deleted in a diploid Candida strain, and heterozygous and homozygous strains, in both Ura ؉ and Ura ؊ backgrounds, were obtained to facilitate the analysis of the function of the gene. Deletion of the two alleles of the MKC1 gene gave rise to viable cells that grew at 28 and 37؇C but, nevertheless, displayed a variety of phenotypic traits under more stringent conditions. These included a low growth yield and a loss of viability in cultures grown at 42؇C, a high sensitivity to thermal shocks at 55؇C, an enhanced susceptibility to caffeine that was osmotically remediable, and the formation of a weak cell wall with a very low resistance to complex lytic enzyme preparations. The analysis of the functions downstream of the MKC1 gene should contribute to understanding of the connection of growth and morphogenesis in pathogenic fungi.

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“…In Candida species, the codon CUG is known to be translated as serine instead of leucine by the tRNA CAG Ser responsible for this non-universal decoding (45,46). We previously reported that this tRNA CAG Ser can be aminoacylated not only with serine, but also with leucine to some extent in vitro as well as in vivo (47).…”

Section: Discussionmentioning

confidence: 99%

Dual Mode Recognition of Two Isoacceptor tRNAs by Mammalian Mitochondrial Seryl-tRNA Synthetase

Shimada¹,

Suzuki²,

Watanabe³

2001

Journal of Biological Chemistry

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Animal mitochondrial translation systems contain two serine tRNAs, corresponding to the codons AGY (Y ‫؍‬ U and C) and UCN (N ‫؍‬ U, C, A, and G), each possessing an unusual secondary structure; tRNA GCU Ser (for AGY) lacks the entire D arm, whereas tRNA UGA Ser (for UCN) has an unusual cloverleaf configuration. We previously demonstrated that a single bovine mitochondrial seryltRNA synthetase (mt SerRS) recognizes these topologically distinct isoacceptors having no common sequence or structure. Recombinant mt SerRS clearly footprinted at the T⌿C loop of each isoacceptor, and kinetic studies revealed that mt SerRS specifically recognized the T⌿C loop sequence in each isoacceptor. However, in the case of tRNA UGA Ser , T⌿C loop-D loop interaction was further required for recognition, suggesting that mt SerRS recognizes the two substrates by distinct mechanisms. mt SerRS could slightly but significantly misacylate mitochondrial tRNA Gln , which has the same T⌿C loop sequence as tRNA UGA Ser , implying that the fidelity of mitochondrial translation is maintained by kinetic discrimination of tRNAs in the network of aminoacyltRNA synthetases.

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The codon CUG is read as serine in an asporogenic yeast Candida cylindracea

Cited by 222 publications

References 18 publications

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila

Functional Characterization of the MKC1 Gene of Candida albicans, Which Encodes a Mitogen-Activated Protein Kinase Homolog Related to Cell Integrity

Dual Mode Recognition of Two Isoacceptor tRNAs by Mammalian Mitochondrial Seryl-tRNA Synthetase

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