The coelomic epithelium transcriptome from a clonal sea star, Coscinasterias muricata

Gabre, Jonatan L.; Martı́nez, Pedro; Sköld, Helén Nilsson; Ortega‐Martinez, Olga

doi:10.1016/j.margen.2015.07.010

Cited by 9 publications

(11 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In parallel to the mentioned proteomic studies, other global (high-throuput) approaches, using the analysis of transcriptomes and/or genomes, are starting to be used. These approaches give us an unprecedented view at biological processes, such as regeneration, and how their regulation is effected at the transcriptional level in other asteroids (Gabre et al, 2015;Semmens et al, 2016;Hall et al, 2017).…”

Section: Asteroidsmentioning

confidence: 99%

Regeneration in Stellate Echinoderms: Crinoidea, Asteroidea and Ophiuroidea

Khadra

Sugni

Ferrario

et al. 2018

Results and Problems in Cell Differentiation

Self Cite

View full text Add to dashboard Cite

Reparative regeneration is defined as the replacement of lost adult body parts and is a phenomenon widespread yet highly variable among animals. This raises the question of which key cellular and molecular mechanisms have to be implemented in order to efficiently and correctly replace entire body parts in any animal. To address this question, different studies using an integrated cellular and functional genomic approach to study regeneration in stellate echinoderms (crinoids, asteroids and ophiuroids) had been carried out over the last few years. The phylum Echinodermata is recognized for the striking regeneration potential shown by the members of its different clades. Indeed, stellate echinoderms are considered among the most useful and tractable experimental models for carrying comprehensive studies focused on ecological, developmental and evolutionary aspects. Moreover, most of them are tractable in the laboratory and, thus, should allow us to understand the underlying mechanisms, cellular and molecular, which are involved. Here, a comprehensive analysis of the cellular/histological components of the regenerative process in crinoids, asteroids and ophiuroids is described and compared. However, though this knowledge provided us with some clear insights into the global distribution of cell types at different times, it did not explain us how the recruited cells are specified (and from which precursors) over time and where are they located in the animal. The precise answer to these queries needs the incorporation of molecular approaches, both descriptive and functional. Yet, the molecular studies in stellate echinoderms are still limited to characterization of some gene families and protein factors involved in arm regeneration but, at present, have not shed light on most of the basic mechanisms. In this context, further studies are needed specifically to understand the role of regulatory factors and their spatio-temporal deployment in the growing arms. A focus on developing functional tools over the next few years should be of fundamental importance.

show abstract

Section: Asteroidsmentioning

confidence: 99%

Regeneration in Stellate Echinoderms: Crinoidea, Asteroidea and Ophiuroidea

Khadra

Sugni

Ferrario

et al. 2018

Results and Problems in Cell Differentiation

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…However, proteomics investigations focused on echinoderm CE are quite limited. Specific molecular information is due to the works by Gabre et al ( 2015 ) and by Kim et al ( 2018 ), which are focused on the characterization of the CE transcriptome from the clonal starfish Coscinasterias muricata and the blue bat starfish Patiria pectinifera , respectively. The analysis of the biological processes associated with the transcripts identified in these studies suggests the involvement of CE in the development of anatomical units.…”

Section: Introductionmentioning

confidence: 99%

More than a simple epithelial layer: multifunctional role of echinoderm coelomic epithelium

et al. 2022

View full text Add to dashboard Cite

In echinoderms, the coelomic epithelium (CE) is reportedly the source of new circulating cells (coelomocytes) as well as the provider of molecular factors such as immunity-related molecules. However, its overall functions have been scarcely studied in detail. In this work, we used an integrated approach based on both microscopy (light and electron) and proteomic analyses to investigate the arm CE in the starfish Marthasterias glacialis during different physiological conditions (i.e., non-regenerating and/or regenerating). Our results show that CE cells share both ultrastructural and proteomic features with circulating coelomocytes (echinoderm immune cells). Additionally, microscopy and proteomic analyses indicate that CE cells are actively involved in protein synthesis and processing, and membrane trafficking processes such as phagocytosis (particularly of myocytes) and massive secretion phenomena. The latter might provide molecules (e.g., immune factors) and fluids for proper arm growth/regrowth. No stem cell marker was identified and no pre-existing stem cell was observed within the CE. Rather, during regeneration, CE cells undergo dedifferentiation and epithelial-mesenchymal transition to deliver progenitor cells for tissue replacement. Overall, our work underlines that echinoderm CE is not a “simple epithelial lining” and that instead it plays multiple functions which span from immunity-related roles as well as being a source of regeneration-competent cells for arm growth/regrowth.

show abstract

“…With the intense interest in stem cells in the invertebrate deuterostomes [44], particularly with respect to neural regeneration, our results highlight the importance to consider regeneration post-autotomy in seastars as a fruitful model system. Neural regeneration in C. muricata provides an interesting system to study CNS regeneration in deuterostomes for biomedical research, especially in parallel with molecular investigations [45,46] to generate insights into the cellular and genetic mechanisms underlying neural regeneration in echinoderms.…”

Section: Discussionmentioning

confidence: 99%

Expression of the neuropeptide SALMFamide-1 during regeneration of the seastar radial nerve cord following arm autotomy

et al. 2019

View full text Add to dashboard Cite

Arm loss through a separation at a specialized autotomy plane in echinoderms is inextricably linked to regeneration, but the link between these phenomena is poorly explored. We investigated nervous system regeneration post-autotomy in the asteriid seastar Coscinasterias muricata , focusing on the reorganization of the radial nerve cord (RNC) into the ectoneural neuroepithelium and neuropile, and the hyponeural region, using antibodies to the seastar-specific neuropeptide SALMFamide-1 (S1). Parallel changes in the associated haemal and coelomic vessels were also examined. A new arm bud appeared in 3–5 days with regeneration over three weeks. At the nerve stump and in the RNC immediately behind, the haemal sinus/hyponeural coelomic compartments enlarged into a hypertrophied space filled with migratory cells that appear to be involved in wound healing and regeneration. The haemal and coelomic compartments provided a conduit for these cells to gain rapid access to the regeneration site. An increase in the number of glia-like cells indicates the importance of these cells in regeneration. Proximal to the autotomy plane, the original RNC exhibited Wallerian-type degeneration, as seen in disorganized axons and enlarged S1-positive varicosities. The imperative to regrow lost arms quickly is reflected in the efficiency of regeneration from the autotomy plane facilitated by the rapid appearance of progenitor-like migratory cells. In parallel to its specialization for defensive arm detachment, the autotomy plane appears to be adapted to promote regeneration. This highlights the importance of examining autotomy-induced regeneration in seastars as a model system to study nervous system regeneration in deuterostomes and the mechanisms involved with the massive migration of stem-like cells to facilitate rapid recovery.

show abstract

The coelomic epithelium transcriptome from a clonal sea star, Coscinasterias muricata

Cited by 9 publications

References 5 publications

Regeneration in Stellate Echinoderms: Crinoidea, Asteroidea and Ophiuroidea

Regeneration in Stellate Echinoderms: Crinoidea, Asteroidea and Ophiuroidea

More than a simple epithelial layer: multifunctional role of echinoderm coelomic epithelium

Expression of the neuropeptide SALMFamide-1 during regeneration of the seastar radial nerve cord following arm autotomy

Contact Info

Product

Resources

About