The coexistence of monopartite integrative and conjugative elements in the genomes of Acidobacteria

Gonçalves, Osiel Silva; Santana, Mateus Ferreira

doi:10.1016/j.gene.2021.145476

Cited by 17 publications

(8 citation statements)

References 37 publications

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“…In the same way as in our results, the predilection of this bacterial group for the bulk soil has also been described in comparison with the rhizosphere ( Kielak et al, 2016 ; Conradie and Jacobs, 2020 ). Several authors have also discussed the potential of this bacterial class for the degradation of various pollutants ( Feng et al, 2021 ; Gonçalves and Santana, 2021 ) and its potential biotechnological application in Hg degradation ( Vishnivetskaya et al, 2011 ; McDaniel et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Metagenomic Study of Rhizospheric and Bulk Mercury-Contaminated Soils in the Mining District of Almadén

et al. 2022

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Soil contamination by heavy metals, particularly mercury (Hg), is a problem that can seriously affect the environment, animals, and human health. Hg has the capacity to biomagnify in the food chain. That fact can lead to pathologies, of those which affect the central nervous system being the most severe. It is convenient to know the biological environmental indicators that alert of the effects of Hg contamination as well as the biological mechanisms that can help in its remediation. To contribute to this knowledge, this study conducted comparative analysis by the use of Shotgun metagenomics of the microbial communities in rhizospheric soils and bulk soil of the mining region of Almadén (Ciudad Real, Spain), one of the most affected areas by Hg in the world The sequences obtained was analyzed with MetaPhlAn2 tool and SUPER-FOCUS. The most abundant taxa in the taxonomic analysis in bulk soil were those of Actinobateria and Alphaproteobacteria. On the contrary, in the rhizospheric soil microorganisms belonging to the phylum Proteobacteria were abundant, evidencing that roots have a selective effect on the rhizospheric communities. In order to analyze possible indicators of biological contamination, a functional potential analysis was performed. The results point to a co-selection of the mechanisms of resistance to Hg and the mechanisms of resistance to antibiotics or other toxic compounds in environments contaminated by Hg. Likewise, the finding of antibiotic resistance mechanisms typical of the human clinic, such as resistance to beta-lactams and glycopeptics (vancomycin), suggests that these environments can behave as reservoirs. The sequences involved in Hg resistance (operon mer and efflux pumps) have a similar abundance in both soil types. However, the response to abiotic stress (salinity, desiccation, and contaminants) is more prevalent in rhizospheric soil. Finally, sequences involved in nitrogen fixation and metabolism and plant growth promotion (PGP genes) were identified, with higher relative abundances in rhizospheric soils. These findings can be the starting point for the targeted search for microorganisms suitable for further use in bioremediation processes in Hg-contaminated environments.

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Section: Discussionmentioning

confidence: 99%

Comparative Metagenomic Study of Rhizospheric and Bulk Mercury-Contaminated Soils in the Mining District of Almadén

et al. 2022

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“…In terms of microbial communities, Acidobacteria had the greatest influence on the resistomes in RCWs and their adjacent estuaries. Coexistence of multiples integrative and conjugative elements has been found in the chromosome of Acidobacteria (Goncalves and Santana, 2021). Therefore, they could easily become resistant to antibiotics and contribute to the dissemination of ARGs, mediated by the MGEs in estuaries and RCWs.…”

Section: Relationships Among Bacteria Communities Mobile Genetic Elem...mentioning

confidence: 99%

Occurrence and driving mechanism of antibiotic resistance genes in marine recreational water around Qinhuangdao, China

Han

Wen

et al. 2022

Front. Mar. Sci.

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Rapid urbanization and other human activities have exacerbated the global human health risks associated with antibiotic resistance genes (ARGs). Swimming in contaminated waters is one of important pathways by which humans can be exposed to ARGs. However, little is currently known about the overall levels of ARGs in recreational waters and the forces driving their occurrence. In this study, we analyzed the ARGs and bacterial communities in water samples taken from five recreational coastal waters (RCWs) and their adjacent estuaries in the Qinhuangdao area of Bohai Bay, China, using highthroughput quantitative PCR and 16S rRNA gene amplicon sequencing technique. The relative abundances of ARGs in RCWs varied greatly, and occurred at levels close to, or even higher than, those in the neighboring estuaries, ranging from 9.4×10 -2 to 2.5 copies/16S rRNA gene. A total of 159 different ARGs able to express resistance to almost all antibiotics were detected in all of the water samples. b-lactamase resistance genes (blaTEM gene) were the most abundance of all ARGs. Patterns of ARG occurrence were significantly correlated with those of bacterial communities (Mantel test, P < 0.05). A partial redundancy analysis showed that differences in bacterial communities accounted for 50.3% of the total ARG variations, which was much higher than the sum of the contribution of mobile genetic elements (18.1%) and the other selected environmental factors (8.3%). Principal coordinate analysis based on Bray-Curtis distance revealed the similarities between ARG profiles in the RCWs and in their adjacent estuaries. To our knowledge, this is the first study to report the ARG profiles in RCWs using high throughput qPCR technology. These results implied that it is necessary to develop proper regulations and monitoring strategies of RCWs for reducing risks of ARGs on human health. KEYWORDS antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), bacterial community, recreational coastal water, human public health Frontiers in Marine Science frontiersin.org 01

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“…These classes included Methylomirabilota, Acidobacteriota, Sumerlaeota, MBNT15, Planctomycetota, Chloroflexi, Gemmatimonadota, Myxococcota, Dependentiae, Entotheonellaeota, RCP2-54, SAR324_clade_Marine_group_B, Latescibacterota, Armatimonadota, and NB1-j. The genome of Acidobacteria members contains several genes associated with active molecules, which play an important role in the transmission of antibiotic resistance [ 60 ]. However, due to a lack of understanding of the physiological properties of Acidobacteria, the mechanism by which earthworm gut digestion reduces ARG is not clear.…”

Section: Resultsmentioning

confidence: 99%

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

Chen

Zhang

et al. 2022

IJERPH

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Antibiotic resistance pollution in livestock manure is a persistent issue that has drawn public attention. Vermicomposting is an ecofriendly biological process that can render livestock manure harmless and resourceful. However, little is known about the impact of vermicomposting on antibiotic resistance in livestock manure under stress caused by potentially toxic arsenic levels. Herein, lab-scale vermicomposting was performed to comprehensively evaluate the shift in antibiotic resistance genes (ARGs) and related microorganisms in fresh earthworm casts as well as vermicompost product health (i.e., nutrient availability and enzyme activity) when they were fed on arsenic-contaminated cow manure. The results showed that the earthworms’ interaction with cow dung led to a significant reduction in ARG concentrations, especially for tetracycline ARGs (tet-ARGs), β-lactam ARGs (bla-ARGs), and quinolone ARGs (qnr-ARGs). However, arsenic significantly enhanced ARG accumulation in earthworm casts in a dose-dependent manner. Moreover, vermicomposting increased the percentage of Bacteroidota in the converted products. Furthermore, arsenic exposure at low concentrations promoted the proliferation of Proteobacteria, whereas high concentrations had little effect on Proteobacteria. Our study provides valuable insight into the changes in the antibiotic resistome and related microorganisms during vermicomposting of arsenic-amended cow manure, and it is crucial to explain the environmental impact of earthworms and improve our understanding of the reciprocal benefits of soil invertebrates.

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The coexistence of monopartite integrative and conjugative elements in the genomes of Acidobacteria

Cited by 17 publications

References 37 publications

Comparative Metagenomic Study of Rhizospheric and Bulk Mercury-Contaminated Soils in the Mining District of Almadén

Comparative Metagenomic Study of Rhizospheric and Bulk Mercury-Contaminated Soils in the Mining District of Almadén

Occurrence and driving mechanism of antibiotic resistance genes in marine recreational water around Qinhuangdao, China

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

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