The cognitive enhancer T-588 partially compensates the motor associative learning impairments induced by scopolamine injection in mice.

Abstract

The authors studied the effects of T-588 on scopolamine-induced memory impairments in the acquisition of a classical eyeblink conditioning in behaving adult mice. Mice injected with 0.3 mg/kg of scopolamine showed a marked deficit, compared with nontreated mice, in the acquisition of classical eyeblink conditioning using a trace paradigm. Coadministration of T-588 (0.05% wt/vol, in water) with scopolamine (0.3 mg/kg) significantly prevented this deficit in associative learning. To further assess the effects of T-588 on motor coordination and the cognitive deficits induced by scopolamine, the authors compared adult controls or scopolamine-treated mice in different behavioral tasks: rotarod, object recognition, passive avoidance, and prepulse inhibition. In all of these tasks, the authors found a significant impairment in the motor or cognitive abilities in scopolamine-injected mice, compared with controls. In addition, the coadministration of T-588 with scopolamine restored deficits induced by scopolamine alone. Importantly, the administration of T-588 alone did not evoke any change compared with values obtained for controls. These results suggest that T-588 could be used as a pharmacological agent to improve motor and associative learning disorders.

Effects of Enriched Physical and Social Environments on Motor Performance, Associative Learning, and Hippocampal Neurogenesis in Mice

“…The total time that each mouse was able to stay on the rod was recorded automatically by a trip switch under the floor of each rotating drum, and computed as the latency to fall. Results were averaged to obtain a single value for each group and session [38]. The percentage of animals per group able to stay on the rod for ≥300 s without a fall was computed as a criterion.…”

“…For the rotarod test, collected data (latencies to fall, session, and animal) were collected by computer and stored for off-line analysis [38]. For operant conditioning, cumulative records of lever pressings and pellet rewards were stored on-line on a computer connected to the Skinner boxes.…”

Alterations in microglial phenotype and hippocampal neuronal function in transgenic mice with astrocyte-targeted production of interleukin-10

“…For the passive avoidance test and in accordance to a previous study from our group (Eleore et al, 2007), each animal was placed in darkness 5 min before training. Then, mice were placed individually in an illuminated box (10 Â 13 Â 15 cm) connected to a dark box of the same size equipped with an electric grid floor, and separated by an automatic door.…”

Motor-Coordination-Dependent Learning, More than Others, Is Impaired in Transgenic Mice Expressing Pseudorabies Virus Immediate-Early Protein IE180

“…Twenty-four hours after the initial training, mice were tested again, with a new object (C1) and an object identical to the old one (B2). The same procedure was carried out 72 h after the initial training (see [20] for details). The attention index (i.e., the percentage of attention) to each object (familiar or new) was expressed as a percentage of the total attention to the two objects exhibited during each session.…”