The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development

Sorushanova, Anna; Delgado, Luis M.; Wu, Zhuning; Shologu, Naledi; Kshirsagar, Aniket; Raghunath, Rufus; Mullen, Anne Maria; Bayon, Yves; Pandit, Abhay; Raghunath, Michael; Zeugolis, Dimitrios I.

doi:10.1002/adma.201801651

Cited by 772 publications

(645 citation statements)

References 762 publications

(675 reference statements)

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“…Thus far, MMC has been shown to enhance ECM deposition in various permanently differentiated and stem cell populations (41, 42, 47). It is worth noting that in the deposited under MMC conditions collagen exhibits the characteristic d ‐periodicity (41, 42), a strong indicator for a regular self‐assembly of collagen molecules (53). Although MMC has been previously combined with other microenvironmental modulators, such as hypoxia (47, 54), it is yet to be used simultaneously with mechanical stimulation.…”

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confidence: 99%

Multifactorial bottom‐up bioengineering approaches for the development of living tissue substitutes

2019

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Bottom‐up bioengineering utilizes the inherent capacity of cells to build highly sophisticated structures with high levels of biomimicry. Despite the significant advancements in the field, monodomain approaches require prolonged culture time to develop an implantable device, usually associated with cell phenotypic drift in culture. Herein, we assessed the simultaneous effect of macromolecular crowding (MMC) and mechanical loading in enhancing extracellular matrix (ECM) deposition while maintaining tenocyte (TC) phenotype and differentiating bone marrow stem cells (BMSCs) or transdifferentiating neonatal and adult dermal fibroblasts toward tenogenic lineage. At d 7, all cell types presented cytoskeleton alignment perpendicular to the applied load independently of the use of MMC. MMC enhanced ECM deposition in all cell types. Gene expression analysis indicated that MMC and mechanical loading maintained TC phenotype, whereas tenogenic differentiation of BMSCs or transdifferentiation of dermal fibroblasts was not achieved. Our data suggest that multifactorial bottom‐up bioengineering approaches significantly accelerate the development of biomimetic tissue equivalents.—Gaspar, D., Ryan, C. N. M., Zeugolis, D. I. Multifactorial bottom‐up bioengineering approaches for the development of living tissue substitutes. FASEB J. 33, 5741–5754 (2019). http://www.fasebj.org

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confidence: 99%

Multifactorial bottom‐up bioengineering approaches for the development of living tissue substitutes

2019

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“…Collagen is an important protein in animal connective tissue. It is also the main component of extracellular matrix (ECM) and could provide support for cell growth (Sorushanova et al, 2019). Osteogenesis imperfect (OI), also known as brittle bone disease, is a genetic connective tissue disorder with genetic and phenotypic diversity (Friedrich, Scheuer & Holtje, 2019;Subramanian & Viswanathan, 2019).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome for the breast muscle of Jinghai yellow chicken at early growth stages

Zhang

et al. 2020

PeerJ

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Background. The meat quality of yellow feathered broilers is better than the quality of its production. Growth traits are important in the broiler industry. The exploration of regulation mechanisms for the skeletal muscle would help to increase the growth performance of chickens. At present, some progress has been made by researchers, but the molecular mechanisms of the skeletal muscle still remain unclear and need to be improved. Methods. In this study, the breast muscles of fast-and slow-growing female Jinghai yellow chickens (F4F, F8F, F4S, F8S) and slow-growing male Jinghai yellow chickens (M4S, M8S) aged four and eight weeks were selected for transcriptome sequencing (RNA-seq). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected nine DEGs to verify the accuracy of the sequencing by qPCR. Results. The differential gene expression analysis resulted in 364, 219 and 111 DEGs (adjusted P-value ≤ 0.05) for the three comparison groups, F8FvsF4F, F8SvsF4S, and M8SvsM4S, respectively. Three common DEGs (ADAMTS20, ARHGAP19, and Novel00254) were found, and they were all highly expressed at four weeks of age. In addition, some other genes related to growth and development, such as ANXA1, COL1A1, MYH15, TGFB3 and ACTC1, were obtained. The most common DEGs (n = 58) were found between the two comparison groups F8FvsF4F and F8SvsF4S, and they might play important roles in the growth of female chickens. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway also showed some significant enrichment pathways, for instance, extracellular matrix (ECM)-receptor interaction, focal adhesion, cell cycle, and DNA replication. The two pathways that were significantly enriched in the F8FvsF4F group were all contained in that of F8SvsF4S. The same two pathways were ECM-receptor interaction and focal adhesion, and they had great influence on the growth of chickens. However, many differences existed between male and female chickens in regards to common DEGs and KEGG pathways. The results would help to reveal the regulation mechanism of the growth and development of chickens and serve as a guideline to propose an experimental design on gene function with the DEGs and pathways. M. 2017. Sound analysis to model weight of broiler chickens. Francisco CL, Jorge AM, Dalpaisilva M, Carani FR, Cabeço LC, Silva SR. 2011. Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes. Meat Science 88(3):535-541 DOI 10.1016/j.meatsci.2011.02.007. Friedrich RE, Scheuer HA, Holtje W. 2019. The effect of bisphosphonate medication on orthodontics and orthognathic surgery in patients with osteogenesis imperfecta. GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW 8:Doc06 . 2019. Synergistic effects of recombinant Lentiviral-mediated BMP2 and TGF-beta3 on the osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro. Cytokine 120:1-8 Noda M. 2016. Collagens VI and XII form complexes mediating osteoblast interact...

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“…The paraffin base layer of the capsule does not permit cell attachment, which may be a possible requirement for collagen production. Other more complex biomaterials may be needed for more enhanced collagen and the production of other extracellular structures …”

Section: Discussionmentioning

confidence: 99%

3D Culture Histology Cryosectioned Well Insert Technology Preserves the Structural Relationship between Cells and Biomaterials for Time‐Lapse Analysis of 3D Cultures

Charbonneau

Al‐Samadi

Salo

et al. 2019

Biotechnology Journal

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When performing histology of softer biomaterials, aspiration disrupts the cellular and molecular location information. This study aims to develop a cryosectionable well insert able to preserve the biomaterial and cell's original 3D conformation from the well to histology analysis. The well insert is composed of a paraffin‐coated gelatine pill. Within the coated capsule, the human epithelial cell line (NS‐SV‐AC) is cultured in Matrigel, GrowDex, Myogel, Myogel + GrowDex, or cell culture media for 14 days. At 0 and 14 days, the samples are frozen in liquid nitrogen and cryotome is used to create sections. The slides are stained by Sirius Red and immunohistochemistry using antibodies human collagens I–V and human Ki‐67. Sirius Red shows pink shades of biomaterials and the best cellular vertical distribution throughout the sagittal section of the well is achieved with Matrigel, GrowDex, and Myogel + GrowDex; in Myogel and media, the cells sink. For collagen protein expression, only Matrigel induces a notable difference while in the other materials, collagen staining is weak or difficult to distinguish from endogenous collagens. Ki‐67 expression is maintained over time. The 3D‐cryo well insert provides a new time‐lapse histology perspective of analysis for liquid or gel cultures that maintains cells and macromolecules in their unaltered in‐well configuration.

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The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development

Cited by 772 publications

References 762 publications

Multifactorial bottom‐up bioengineering approaches for the development of living tissue substitutes

Multifactorial bottom‐up bioengineering approaches for the development of living tissue substitutes

Transcriptome for the breast muscle of Jinghai yellow chicken at early growth stages

3D Culture Histology Cryosectioned Well Insert Technology Preserves the Structural Relationship between Cells and Biomaterials for Time‐Lapse Analysis of 3D Cultures

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