The Collection Method for Crop Fungal Spores Based on an Efficient Microfluidic Device

Wang, Pan; Yuan, Shuxia; Yang, Ning; Wang, Aiying; Fordjour, Alexander; shengbo, Chen

doi:10.4209/aaqr.2019.08.0424

Cited by 20 publications

(18 citation statements)

References 22 publications

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“…The results indicate that respiratory intensity can be used to predict the spore aerosolization of A. niger colonies. Although some previous studies have conducted experimental tests to determine the release potential of fungal spores, the predicted spore release rate was inaccurate (Gorny et al, 2001;Kesavan et al, 2019;Wang et al, 2020). One reason is that the growth age has a significant effect on spore detachment (Sivasubramani et al, 2004;Frankel et al, 2014).…”

Section: Prediction Of the Spore Release Proportion For Colonies With Heterogeneous Agesmentioning

confidence: 99%

Prediction of Aerosolized Fungal Spores by Determining Respiratory Intensity of the Growing Colony

Wang

Zhang

et al. 2020

Aerosol Air Qual. Res.

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Aerosolization of fungal spores from moldy surfaces can cause adverse health effects. The spores of an older colony are more easily detached than those of a younger colony. When the growth age of a colony is known, the aerosolization of spores can be more accurately predicted. This study proposed the adoption of respiratory intensity as an indicator of the growth age of Aspergillus niger colonies. Respiratory intensity is the mass of carbon dioxide produced by the respiration of a colony per unit quantity of spores within a certain time. Two types of colonies, with homogeneous and heterogeneous age distributions, were aerosolized in a wind tunnel to determine the spore release proportion and validate the method. The results revealed that the respiratory intensity of the homogeneous colony is closely correlated with the growth age when the age of the colony is less than 14 days under standard growth conditions. The spore release proportion increases with the growth age and the blowing speed. The predicted spore release proportion for a heterogeneous colony is more precise when the colony is divided into multiple sub-colonies to characterize the growth age for each individual spore release proportion. The relative errors of the predicted spore release proportions as compared with the test values were less than 10%.

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Section: Prediction Of the Spore Release Proportion For Colonies With Heterogeneous Agesmentioning

confidence: 99%

Prediction of Aerosolized Fungal Spores by Determining Respiratory Intensity of the Growing Colony

Wang

Zhang

et al. 2020

Aerosol Air Qual. Res.

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“…Microfluidics has the potential to manipulate bioparticles aimed at focusing, trapping, separating, and sorting particles [11][12][13] . Wang et al [14] designed a microfluidic chip that can directly enrich airborne fungal spores from airflow. The chip includes three parts: half-wave pretreatment channel, inertial impactor and low-pressure collection chamber.…”

Section: Introduction mentioning

confidence: 99%

Separation-enrichment method for airborne disease spores based on microfluidic chip

Zhang

Yang

et al. 2021

International Journal of Agricultural and Biological Engineering

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Airborne diseases are likely to cause crop yield reduction, which has aroused widespread concern. In this study, a two-stage separation-enrichment structure microfluidic chip with a compound field for separation and enrichment of the greenhouse crops airborne disease spores directly from gas flow was developed. The chip is mainly composed of three parts: arc structure pretreatment channel, semicircular electrode structure and collection tank. COMSOL 5.1 software was used to simulate the designed microfluidic chip. 30 µm particles were used to represent P. xanthii spores, 25 µm particles were used to represent P. cubensis spores, and 16 µm particles were used to represent B. cinerea spores. The simulation results showed that the separation and enrichment efficiency of 16 μm particles, 25 μm particles, and 30 μm particles was 88%, 91%, and 94%, respectively. The experimental verification results were observed under a microscope. The results showed that the separation and enrichment efficiency of B. cinereal spores, P. cubensis spores and P. xanthii spores was 75.7%, 83.8% and 89.4%, respectively. As a result, the designed microfluidic chip can be used to separate and enrich the spores of airborne diseases of greenhouse crops, which can provide a basis for the research of real-time monitoring technology for greenhouse airborne diseases.

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“…Xu et al observed the fine particle behavior successfully in microfluidic chip when it passed a triangular cylinder in a laminar airflow (Xu et al, 2013). Wang et al replaced the impaction plate by a low-pressure collection chamber which eliminates the re-entrainment and bounce effects of particles, and finally confirmed a satisfied collection efficiency (Wang et al, 2020). However, most of the abovementioned studies does not include the improvement of the collection efficiency curve.…”

Section: Introductionmentioning

confidence: 99%

Performance Evaluation of a Virtual Impactor with an Additional Pretreatment Structure for Particle Separation

Wang

Yuan

Yang

et al. 2021

Aerosol Air Qual. Res.

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The Collection Method for Crop Fungal Spores Based on an Efficient Microfluidic Device

Cited by 20 publications

References 22 publications

Prediction of Aerosolized Fungal Spores by Determining Respiratory Intensity of the Growing Colony

Prediction of Aerosolized Fungal Spores by Determining Respiratory Intensity of the Growing Colony

Separation-enrichment method for airborne disease spores based on microfluidic chip

Performance Evaluation of a Virtual Impactor with an Additional Pretreatment Structure for Particle Separation

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