The Colorimetric Determination of Cholesterol

Sperry, Warren M.; Brand, Florence C.

doi:10.1016/s0021-9258(18)72150-9

Cited by 107 publications

(7 citation statements)

References 12 publications

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“…In the ceramides there is a predominance of short-chain saturated normal fatty (33). In these sphingolipids there is a ler percentage of monounsaturated acids smalfatty acids in HFA than in NFA (20,27,34). The distributions of fatty acid species in the brain cerebrosides and sulfatides of both groups of rats are comparable in general pattern.…”

Section: Chemical Studiesmentioning

confidence: 85%

“…Individual phospholipids were resolved by thin-layer chromatography (TLC) (18) with Silica Gel H and quantitated by phosphorus content (19). Cholesterol was determined by using the method of Sperry and Brand (20), and cerebrosides and sulfatides by the orcinol reaction (21). Protein and DNA were extracted from the brain tissue (22) and measured by the methods of Lowry et al (23) and Burton (24), respectively.…”

Section: Morphometric Proceduresmentioning

confidence: 99%

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Effect of Lead Intoxication on the Postnatal Growth of the Rat Nervous System

Krigman¹,

Hogan²

1974

Environ Health Perspect

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Lead encephalopathy was induced in developing Long-Evans rats by adding lead carbonate (4% w/w) to the diet of nursing mother immediately after delivery. The morphological and biochemical features of cerebral ontogenesis were studied in 30-day-old rats. By the 30th postnatal day, the overall effect of lead intoxication was retardation of brain growth. The mass of both the cerebral gray and white matter was appreciably reduced in the lead rats without any reduction in cell populations. While the neuronal population was preserved, the growth of neurons was reduced and their maturation retarded. The retarded neuronal growth was characterized by the limited proliferation of processes in the neuropil and by the reduction in the number of synapses per neuron. However, synaptogenesis was neither delayed nor perturbed but reduced by the limited development of neuronal dendritic fields. The myelination was altered and its cerebral content significantly reduced. The effect of lead on myelination was one of hypomyelination. The hypomyelination appears to be primarily related to retarded growth and maturation of the neuron and is not a reflection of a defect in the myelinating glia or a delay in the initiation of myelination. ImagesFIGURE 1.

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Section: Chemical Studiesmentioning

confidence: 85%

Section: Morphometric Proceduresmentioning

confidence: 99%

Effect of Lead Intoxication on the Postnatal Growth of the Rat Nervous System

Krigman¹,

Hogan²

1974

Environ Health Perspect

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“…The work of Hoffman (1940), as extended and discussed here, has clarified this position, and Sperry & Brand (1943), reviewing work on this reaction, stated that in their opinion the green colour was entirely empirical, no doubt implying that it was most variable, and that it was dependent I952 principally upon the factors of time and temperature, whereas the brown colour (described here as the yellow colour), appeared to be the end product and might be proportional to the amount of cholesterol. The present work has confirmed this view, because it has been shown that a relationship exists between the absorptions in the red and blue regions of the spectrum such that a constant can be obtained which is proportional to the amount of cholesterol present.…”

Section: Discussionmentioning

confidence: 57%

The determination of cholesterol by the Liebermann-Burchard reaction

Kenny

1952

Biochemical Journal

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The demonstration of a lactosephosphate on the other hand, does bring more certain evidence for the view, hitherto held with very slender experimental support, that phosphorylation is of fundamental importance in lactose synthesis. An isolated instance of the occurrence of such an ester in a trichloroacetic acid extract of cow-udder tissue has already been reported (Malpress, 1950), and it seems probable that this compound may prove to be the immediate precursor of lactose in the mammay gland. SUMMARY 1. Barium fractionation procedures, supplemented by chromatographic analysis, have shown that ax-galactose-1-phosphate, c( ?)-glucose-1-phosphate, glucose-6(?)-phosphate, a lactosephosphate and phosphopyruvic acid are present in trace amounts in normal milk.2. The pomsible significance of these findings in relation to lactose synthesis is discu#sed.Our thanks are due to Mr J. G. Murray (Department of Agricultural Bacteriology) for assistance in obtaining milk samples, and for bacteriological examinations; and to Prof. D. C. Harrison for many valuable discussions.

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“…However, the low cholesterol level causes hepatopathy and anemia . Different techniques such as high-pressure liquid chromatography, mass spectroscopy, calorimetry, spectrophotometry, fluorimetry, electrochemical electrochemistry, and polarography are used to detect cholesterol level. ,− However, these methods have shortcomings of low specificity, reagent instability, high cost, complexity, and expensive instrumentation. Most of the reported cholesterol-sensing procedures involve the use of the enzyme cholesterol oxidase with a catalytic oxidation process of the cholesterol. , The peroxidase-like activity of copper nanoclusters has also been used for developing a chemiluminescence-based cholesterol sensor.…”

Section: Introductionmentioning

confidence: 99%

“…Different techniques such as high-pressure liquid chromatography, mass spectroscopy, calorimetry, spectrophotometry, fluorimetry, electrochemical electrochemistry, and polarography are used to detect cholesterol level. ,− However, these methods have shortcomings of low specificity, reagent instability, high cost, complexity, and expensive instrumentation. Most of the reported cholesterol-sensing procedures involve the use of the enzyme cholesterol oxidase with a catalytic oxidation process of the cholesterol. , The peroxidase-like activity of copper nanoclusters has also been used for developing a chemiluminescence-based cholesterol sensor. However, the enzymatic method has the shortcomings of high cost, enzymatic denaturation, and low-temperature requirement for storage. , High sensitivity, rapidness, and cost-effectiveness make optical sensors an alternative route to detect cholesterol levels based on analysis of the changes in the luminescence spectrum. , …”

Section: Introductionmentioning

confidence: 99%

Nanocomposite of CuInS/ZnS and Nitrogen-Doped Graphene Quantum Dots for Cholesterol Sensing

et al. 2021

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In this paper, nitrogen graphene quantum dots (N-GQDs) and copper indium sulfide/zinc sulfide (CIS/ZnS) QDs were synthesized via facile hydrothermal and aqueous solution routes, respectively. Herein, a fluorescent nanocomposite has been synthesized between N-GQDs and CIS/ZnS QDs in an aqueous phase. This nanocomposite was characterized by photoluminescence, Raman, and ultraviolet–visible (UV–vis) spectroscopies, high-resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD). This fluorescent nanocomposite was developed as a highly sensitive, selective nonenzymatic cholesterol optical biosensor in 0.312–5 mM cholesterol. HRTEM micrographs confirmed the preparation of CIS/ZnS QDs and N-GQDs with average diameters of 3 and 5 nm, respectively. The as-prepared NG/CIS/ZnS QD nanocomposite had a high sensitivity for cholesterol with a wide linear range of concentration of 0.312–5 mM with an excellent correlation coefficient (R 2) of 0.9688 and limit of detection (LOD) of 0.222 mM.

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The Colorimetric Determination of Cholesterol

Cited by 107 publications

References 12 publications

Effect of Lead Intoxication on the Postnatal Growth of the Rat Nervous System

Effect of Lead Intoxication on the Postnatal Growth of the Rat Nervous System

The determination of cholesterol by the Liebermann-Burchard reaction

Nanocomposite of CuInS/ZnS and Nitrogen-Doped Graphene Quantum Dots for Cholesterol Sensing

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