The colorimetric determination of phosphorus

King, E. J.

doi:10.1042/bj0260292

Cited by 1,578 publications

(328 citation statements)

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“…The bacterial isolate formed the clarification halos on plates, which indicated phosphate solubilization and it was confirmed by testing the changes of pH in broth culture medium on daily basis [12]. To determine the organic acids production in culture medium, B. megaterium mj1212 was inoculated into tricalcium phosphate medium (pH 7.0) and cultured at 35°C, and filtreted through 0.22 lm Millipore filter and 10 ll of filtrates were injected to High Performance Liquid Chromatography (Model: Waters 600E) equipped with a Refractive Index Detector (Model: Waters 410) and RSpak KC-811 column (8.0 9 300 mm) at 40°C.…”

Section: Methodsmentioning

confidence: 82%

Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

et al. 2014

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The current study was conducted to explore the potential of a phosphate solubilizing soil bacterium, Bacillus megaterium mj1212 for enhancing the growth of mustard plants. The newly isolated bacterial strain mj1212 was identified as B. megaterium using phylogenetic analysis and, its phosphate solubilization ability was shown by the clear zone formation on National Botanical Research Institute's Phosphate medium. Moreover, the phosphate solubilization ability of B. megaterium mj1212 was enhanced by optimal culture conditions at pH 7.0 and 35°C which might be due to the presence of malic and quinic acid in the culture medium. The beneficial effect of B. megaterium mj1212 in mustard plants was determined by an increasing shoot length, root length and fresh weight of plants. In the biochemical analysis revealed that chlorophyll, sucrose, glucose, fructose and amino acids (Asp, Thr, Ser, Glu, Gly, Ala, Cys, Val, Met, Ilu, Leu, Tyr, Phe, Lys, His, Arg and Pro) were higher in B. megaterium mj1212 treated plants, when compared to their control. The result of present study suggests that B. megaterium mj1212 treatment could be act as phosphate biofertilizer to improve the plant growth.

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Section: Methodsmentioning

confidence: 82%

Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

et al. 2014

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“…(Heimberg et al, 1965). Triacylglycerol (Van Handel & Zilversmit, 1957;Newman et al, 1961), non-esterified and esterified cholesterol (Rudel & Morris, 1973) and phospholipid (King, 1932) of VLD lipoprotein were analysed. Significance of the differences between groups was determined by Student's t test (Steel & Torrie, 1960).…”

Section: Methodsmentioning

confidence: 99%

Relationship between activity of hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase and secretion of very-low-density-lipoprotein cholesterol by the isolated perfused liver and in the intact rat

Goh¹,

Heimberg²

1979

Biochemical Journal

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The hepatic output of triacylglycerol and cholesterol from very-low-density lipoprotein (VLD lipoprotein), and the activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase were compared in the isolated perfused rat-liver preparation and in the intact rat. The output of triacylglycerol and cholesterol from VLD lipoprotein by the perfused liver was stimulated by oleate concomitant with stimulation of hepatic microsomal hydroxymethylglutaryl-coenzyme A reductase activity. In the intact animal treated with Triton WR-1339, the magnitude of secretion of triacylglycerol and cholesterol from VLD lipoprotein coincided with the diurnal rhythm of hepatic hydroxymethylglutaryl-coenzyme A reductase activity, which was maximal at 24:00h and minimal at 12:00h. These observations suggest that the stimulation of the reductase and of the secretion of cholesterol from VLD lipoprotein by non-esterified fatty acids, as observed with the isolated perfused rat liver preparation in vitro, may also be an important physiological mechanism in vivo. Hepatic cholesterogenesis may be stimulated under conditions conducive to the secretion of the VLD lipoprotein, the primary transport form for triacylglycerol in the postabsorptive state.We have reported previously that oleate stimulated the secretion of total triacylglycerol and total cholesterol by the isolated perfused rat liver in vitro.

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“…To quantitate the amount of ATP per ml, 0.1 ml portions of the Sepharose were first oxidized by suspension in 1 ml of 7% perchloric acid/4.5 M H2SO4 and incubation for 60 min at 2400. The liquid was then analyzed for total phosphorus (11). In our preparations, 1 ml of Sepharose contained 2 /Amol of ATP.…”

Section: Methodsmentioning

confidence: 99%

Binding of ATP to the progesterone receptor.

Moudgil¹,

Toft²

1975

Proc. Natl. Acad. Sci. U.S.A.

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The An initial step in the mechanism of steroid hormone action involves binding of the steroid to receptor proteins in the target cell. The resulting complex then migrates into the cell nucleus and is thought to act as a modulator of gene expression (for review, see refs. 1-3). However, the actual biochemical function of the receptor remains unknown. This is the case because little is known about the regulation of gene activity in higher organisms, and also, because of difficulties in the purification and characterization of the receptor proteins. Some insight into the function of steroid receptors might be gained by studying their ability to interact with various cellular constituents. This approach has been used with the avian progesterone receptor to demonstrate its ability to bind to DNA and chromatin (4). Possible interactions between the receptor and other cellular constituents are difficult to analyze, particularly if the ligand is of a common type and of low molecular weight. Highly purified receptor preparations are not yet available for direct binding studies.We have used affinity chromatography as a relatively unambiguous and direct procedure for measuring the possible interaction of progesterone receptors with various nucleotides. While a role of nucleotides in the initial steps of hormone action is not directly evident, previous studies (5-7) indicate: (i) that energy-requiring or enzymatic steps may be involved in activation of the hormone-receptor complex and its translocation to nuclear sites of actions, and (ii) that this nuclear action is closely associated with the process of RNA synthesis. In addition, there have been a few reports that implicate a relationship between steroid receptors and ATP (see Discussion).The present results show a specific binding between the progesterone receptor and ATP, suggesting an involvement of this nucleotide in some aspect of receptor function. MATERIALS AND METHODSAll reagents were of analytical grade and were made up in glass-distilled water. All ATP was oxidized with sodium metaperiodate according to the method of Gilham (10). To 10 ml of Sepharose-hydrazide preparation in 0.1 M sodium acetate buffer (pH 5) were added 70 ,umol of periodate-oxidized ATP in a total volume of 25 ml of 0.1 M\I sodium acetate, pH 5.0 (8). The suspension was placed in a shaker for 3 hr at 40, after which 2 M NaCl (75 ml) was added and shaking was continued for another 30 min. This suspension was filtered under reduced pressure and washed with 1.0 liter of distilled water and 10Q ml of TETG 901 Abbreviation: TETG, 50 mM Tris-HCl, 1 mM EDTA, 12 mM monothioglycerol (pH 8).

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The colorimetric determination of phosphorus

Cited by 1,578 publications

References 2 publications

Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

Relationship between activity of hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase and secretion of very-low-density-lipoprotein cholesterol by the isolated perfused liver and in the intact rat

Binding of ATP to the progesterone receptor.

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