The combination of astragalus membranaceus and ligustrazine ameliorates micro-haemorrhage by maintaining blood–brain barrier integrity in cerebrally ischaemic rats

Cai, Jun; Pan, Ruihuan; Jia, Xiang; Li, Yue; Hou, Zhiyong; Huang, Run‐Yue; Chen, Xin; Huang, Shiying; Yang, Guo‐Yuan; Sun, Jingbo; Huang, Yan

doi:10.1016/j.jep.2014.10.019

Cited by 21 publications

(20 citation statements)

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“… 16 , 17 The herb has been utilized to treat cerebral ischemia 18 , 19 and maintain the integrity of the blood–brain barrier. 20 Its application was also effective in the therapy of diabetic nephropathy, and by ameliorating the function of kidneys, it caused a reduction in urine protein excretion. 21 , 22 Clinically, Huangqi was reported to reverse memory impairment in diabetic patients.…”

Section: Discussionmentioning

confidence: 99%

Effects of <em>Astragalus</em> polysaccharides on memory impairment in a diabetic rat model

Dun¹,

Liu²,

Qiu³

et al. 2016

NDT

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ObjectiveAstragalus polysaccharides (APS) are active constituents of Astragalus membranaceus. In this study, we aimed to investigate the effects of APS on memory impairment in a diabetic rat model and their mechanisms.MethodsA diabetic model was established in 50 male Wistar rats with streptozotocin intra-peritoneal injection. A blood glucose level higher than 16.7 mmol/L obtained 72 hours after the injection was regarded as a successful diabetic model. The modeled rats were divided into model group, high, medium, and low doses of APS, and piracetam groups (positive control). A group of ten rats without streptozotocin-induced diabetes were used as a normal control. After respective consecutive 8-week treatments, the levels of blood fasting plasma glucose, insulin, hemoglobin A1c, memory performance, hippocampal malondialdehyde, and superoxide dismutase were determined.ResultsAfter the 8-week APS treatment, serum fasting plasma glucose, hemoglobin A1c, and insulin levels were decreased compared with those of the model group (P<0.05). Importantly, memory impairment in the diabetic model was reversed by APS treatments. In addition, hippocampal malondialdehyde concentration was lowered, whereas that of superoxide dismutase was higher after APS treatments.ConclusionAPS are important active components responsible for memory improvement in rats with streptozotocin-induced diabetes. The potential mechanism of action is associated with the effects of APS on glucose and lipid metabolism, and antioxidative and insulin resistance. APS are constituents of A. membranaceus that are potential candidate therapeutic agents for the treatment of memory deficit in diabetes.

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Section: Discussionmentioning

confidence: 99%

Effects of <em>Astragalus</em> polysaccharides on memory impairment in a diabetic rat model

Dun¹,

Liu²,

Qiu³

et al. 2016

NDT

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“…Cellular samples were lysed with NP40 lysis buffer (P0013F; Beyotime, China) and then subjected to SDS‐PAGE and immunoblotting as previously described (Cai et al., ). Primary antibodies against Bax (M1312; HuaAn Biotechnology, China), Bcl‐2 (ab692, Abcam), cleaved caspases‐3 (ab2302; Abcam), GAPDH (ab8245; Abcam) were used.…”

Section: Methodsmentioning

confidence: 99%

Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke

Xie

Cai

et al. 2018

Brain and Behavior

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Background and PurposeCurcumin, a natural antioxidant isolated from Curcuma longa, has been reported to exert neuroprotective effect in animal models of ischemic stroke. However, the underlying mechanism is still not fully understood. The purpose of this study was to investigate the effect of curcumin treatment on neuronal apoptosis in the periinfarct cortex after cerebral ischemia/reperfusion (I/R) injury and in mouse N2a cells after oxygen‐glucose deprivation/reoxygenation (OGD/R) injury and its underlying mechanism.MethodsThe cerebral I/R injury was established by 1‐hr middle cerebral artery occlusion (MCAO) and reperfusion in mice. Infarct volume was determined by TTC staining, and neurological score was evaluated by mNSS. Cell morphology in the ischemic boundary zone were detected by HE staining. The number and apoptotic rate of neurons in ischemic boundary zone were assayed by immunohistochemistry and TUNEL, respectively. Mouse neuroblastoma N2a cells were subjected to OGD/R. Cell viability was assessed with CCK‐8. The mitochondrial membrane potential was measured using JC‐1 staining. The expression of Bax, Bcl‐2, and caspase‐3 was detected using Western blotting. Besides, cellular distribution of Bax was determined by immunofluorescence assays.ResultsCurcumin treatment reduced infarct volume, improved neurological function, alleviated the morphological damage of neurons, and increased neuronal survival rate after I/R injury in vivo. Moreover, curcumin treatment improved cell viability, reduced cell apoptosis, increased Bcl‐2 protein levels while decreased Bax and caspase‐3 expressions in mouse N2a cells after OGD/R injury. Besides, curcumin treatment inhibited Bax activation and maintained mitochondrial membrane integrity.ConclusionCurcumin promotes neuron survival in vivo and in vitro to exert neuroprotective effects against ischemia injury. Moreover, our results for the first time demonstrated curcumin inhibited ischemia‐induced mitochondrial apoptosis via restricting Bax activation, which may be one of the possible mechanisms underlying the neuroprotective effects of curcumin.

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“…The measurement of the surface CBF was conducted by a laser Doppler flowmetry (LDF) (Moor Instruments; Axminster, UK) during the MCAO induction, as previously described [31, 32]. In order to determine the surface CBF that was supplied by the bilateral MCA, 2 burr holes (ID =2 mm) were drilled 2.5 mm to the left and right, and 1.0 mm posterior to bregma, as previously described [31, 32]. When the value of the ipsilateral CBF decreased to 1/3 of the baseline value, the MCA was considered to be successfully occluded.…”

Section: Methodsmentioning

confidence: 99%

Buyang Huanwu decoction facilitates neurorehabilitation through an improvement of synaptic plasticity in cerebral ischemic rats

Pan

Cai

Zhan

et al. 2017

BMC Complement Altern Med

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BackgroundLoss of neural function is a critical but unsolved issue after cerebral ischemia insult. Neuronal plasticity and remodeling are crucial for recovery of neural functions after brain injury. Buyang Huanwu decoction, which is a classic formula in traditional Chinese medicine, can positively alter synaptic plasticity. This study assessed the effects of Buyang Huanwu decoction in combination with physical exercise on neuronal plasticity in cerebral ischemic rats.MethodsCerebral ischemic rats were administered Buyang Huanwu decoction and participated in physical exercise after the induction of a permanent middle cerebral artery occlusion. The neurobehavioral functions and infarct volumes were evaluated. The presynaptic (SYN), postsynaptic (GAP-43) and cytoskeletal (MAP-2) proteins in the coronal brain samples were evaluated by immunohistochemistry and western blot analyses. The ultrastructure of the neuronal synaptic junctions in the same region were analyzed using transmission electron microscopy.ResultsCombination treatment of Buyang Huanwu decoction and physical exercise ameliorated the neurobehavioral deficits (p < 0.05), significantly enhanced the expression levels of SYN, GAP-43 and MAP-2 (p < 0.05), and maintained the synaptic ultrastructure.ConclusionsBuyang Huanwu decoction facilitated neurorehabilitation following a cerebral ischemia insult through an improvement in synaptic plasticity. Graphical abstractThe Buyang Huanwu decoction (BYHWD) combined with physical exercise (PE) attenuates synaptic disruption and promotes synaptic plasticity following cerebral ischemia (stroke). Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1680-9) contains supplementary material, which is available to authorized users.

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The combination of astragalus membranaceus and ligustrazine ameliorates micro-haemorrhage by maintaining blood–brain barrier integrity in cerebrally ischaemic rats

Cited by 21 publications

References 44 publications

Effects of <em>Astragalus</em> polysaccharides on memory impairment in a diabetic rat model

Effects of <em>Astragalus</em> polysaccharides on memory impairment in a diabetic rat model

Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke

Buyang Huanwu decoction facilitates neurorehabilitation through an improvement of synaptic plasticity in cerebral ischemic rats

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