The combination of attenuated Newcastle disease (ND) vaccine with rHVT-ND vaccine at 1 day old is more protective against ND virus challenge than when combined with inactivated ND vaccine

Rauw, Fabienne; Gardin, Yannick; Palya, Vilmos; Berg, Thierry van den; Lambrecht, Bénédicte

doi:10.1080/03079457.2013.859655

Cited by 17 publications

(16 citation statements)

References 29 publications

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“…NDV-specific humoral antibody immune response was evaluated by HI test and NDV-specific IgG, IgM and IgA ELISAs as previously described (Rauw et al, 2009b(Rauw et al, , 2014.…”

Section: Methodsmentioning

confidence: 99%

Quantification of rHVT-F genome load in feather follicles by specific real-time qPCR as an indicator of NDV-specific humoral immunity induced by day-old vaccination in SPF chickens

et al. 2015

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“…NDV-specific humoral antibody immune response was evaluated by HI test and NDV-specific IgG, IgM and IgA ELISAs as previously described (Rauw et al, 2009b(Rauw et al, , 2014.…”

Section: Methodsmentioning

confidence: 99%

Quantification of rHVT-F genome load in feather follicles by specific real-time qPCR as an indicator of NDV-specific humoral immunity induced by day-old vaccination in SPF chickens

et al. 2015

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“…Early vaccination against ND has several advantages, including reduction of competition between field viruses and vaccine development, involvement of high levels of passive immunity and prevention of poor timing of vaccination (Rauw, Gardin, Palya, Van den Berg, & Lambrecht, 2014). There is a trend towards providing in ovo vaccination of chickens embryos with live ND vaccines.…”

Section: Discussionmentioning

confidence: 99%

Effect of adjuvants on in ovo vaccination against Newcastle disease on hatchability, performance and antibody titres in commercial pullets

Roudi

Golian

Haghparast

et al. 2018

Animal Physiology Nutrition

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This study was conducted to investigate the efficacy of in ovo administration of aluminium hydroxide (AH) and/or mannan oligosaccharide (MOS) adjuvants along with lentogenic VG/GA strain-Avinew to alleviate the embryonic pathogenicity of Newcastle disease virus. Six hundred and thirty fertilized Bovans eggs were divided into nine groups of 70 each incubated in a commercial hatchery and administered with eight types of in ovo injections in a factorial design of 2 × 2 × 2 including with/without AH, MOS and Newcastle disease vaccine (NDV), and one uninjected group on day 18 of incubation. Hatchability was higher in the eggs received MOS and/or AH adjuvants plus NDV compared those injected with NDV alone which confirmed the attenuation of NDV. However, the average daily feed intake and feed conversion ratio of pullets hatched from NDV-injected eggs were significantly reduced, but did not affect growth performance during 0-42 days of age. The performance of pullets hatched from eggs injected with AH, MOS or their mixture with NDV was not significantly different during all growth periods. Pullets from MOS + vaccine injected eggs had significantly higher antibody titres against NDV compared to those hatched from either injected with saline or uninjected on d 28 (p < .05). In addition, AH plus vaccine and MOS significantly improved total anti-SRBC and IgG respectively. Histological observation revealed that injection of MOS adjuvant into eggs led to increase crypt depth, whereas AH injection caused a reduction in villus surface area of jejunum in chicks on d 14 post-hatch. It is concluded that in ovo MOS injection as compared to AH may be more effective to attenuate the embryonic pathogenicity of in ovo NDV injection.

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“…In addition, Abdul-Careem et al (2008a) showed agedependent variation in the expression of ChIFNγ mRNA in the spleen after vaccination with HVT. In this study, we developed a recall protocol to measure IBDV-specific ChIFNγ production after ex vivo activation of splenocytes, similar to the approach that we used to measure NDV-specific CMI (Lambrecht et al, 2004;Jakka et al, 2014;Rauw et al, 2014). The first attempts at ELISA-ChIFNγ had low sensitivity and specificity, with a relatively high nonspecific release of ChIFNγ from cells isolated from the unvaccinated group and cultured with live IBDV.…”

Section: Discussionmentioning

confidence: 99%

“…Bivalent recombinant HVT (rHVT) vaccines have been successfully used to induce protection in chickens against several diseases: Newcastle disease (ND) (Reddy et al, 1996;Palya et al, 2012;Rauw et al, 2014), avian influenza (AI) (Rauw et al, 2012;Soejoedono et al, 2012), IBD (Darteil et al, 1995;Tsukamoto et al, 2002;Bublot et al, 2007;Le Gros et al, 2009;Perozo et al, 2009;Lemiere et al, 2011), and infectious laryngotracheitis (Esaki et al, 2013). These rHVT vaccines offer the advantage of inducing an immune response against Marek's disease as well as against a second disease by inserting a foreign gene in the vector that encodes a specific protein to stimulate a protective immune response.…”

Section: Introductionmentioning

confidence: 99%

Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens

et al. 2017

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Infectious bursal disease (IBD) is an avian viral disease that causes severe economic losses in the poultry industry worldwide. The live IBD virus (IBDV) has a potential immunosuppressive effect. Currently available IBDV vaccines have shortcomings, prompting the development of safer and more effective vaccination approaches, including the use of the recombinant turkey herpesvirus vaccine expressing the immunogenic structural VP2 protein of IBDV (recombinant HVT (rHVT)-IBD). The objectives of this study were twofold: (i) to develop in vitro assays and molecular tools to detect the VP2 protein and gene and (ii) to evaluate cell-mediated immunity (CMI) induced by rHVT-IBD vaccination of day-old specific pathogen-free chickens. The VP2 protein expressed by rHVT-IBD-infected chicken embryo fibroblasts was detected using the enzyme-linked immunosorbent assay and immunofluorescence. Using molecular techniques, the VP2 gene was detected in various organs, providing a method to monitor vaccine uptake. rHVT-IBD vaccination induced CMI responses in specific pathogen-free chickens at 5 weeks. CMI was detected by measuring chicken interferon-gamma after ex vivo antigenic stimulation of splenocytes. Moreover, our results showed that the enzyme-linked immunospot approach is more sensitive in detecting chicken interferon-gamma than enzyme-linked immunosorbent assay. The tools developed in this study may be useful in the characterization of new-generation recombinant vaccines and the cellular immune response they induce.

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The combination of attenuated Newcastle disease (ND) vaccine with rHVT-ND vaccine at 1 day old is more protective against ND virus challenge than when combined with inactivated ND vaccine

Cited by 17 publications

References 29 publications

Quantification of rHVT-F genome load in feather follicles by specific real-time qPCR as an indicator of NDV-specific humoral immunity induced by day-old vaccination in SPF chickens

Quantification of rHVT-F genome load in feather follicles by specific real-time qPCR as an indicator of NDV-specific humoral immunity induced by day-old vaccination in SPF chickens

Effect of adjuvants on in ovo vaccination against Newcastle disease on hatchability, performance and antibody titres in commercial pullets

Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens

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