The combination of CASA kinetic parameters and fluorescein staining as a fertility tool  in cryopreserved bull semen

̇İnanç, Muhammed Enes; Çil, Beste; Tekin, Koray; Alemdar, Havva; Daşkin, Ali

doi:10.3906/vet-1801-83

Cited by 17 publications

(9 citation statements)

References 25 publications

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“…Total motility (%), progressive motility (%), curvilinear velocity (VCL, µm/s), average path velocity (VAP, µm/s), straight line velocity (VSL, µm/s), amplitude of lateral head displacement (ALH, µm), beat‐cross frequency (BCF, Hz), linearity (LIN) [(VSL / VCL) × 100], wobble (WOB) [(VAP / VCL) × 100], straightness (STR) [(VSL / VAP) × 100] and hyperactivation [(VCL / VSL) × ALH] were determined. Approximately 400 spermatozoa per sample were evaluated in five microscopic fields (Inanc et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

Quality assessment of frozen bull semen with the precursor A‐kinase anchor protein 4 biomarker

Baştan

Akçay

2021

Andrologia

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In this study, the quality of frozen bull semen was evaluated with the proAKAP4 level test. Sixty straws of frozen bull semen from various batches (n = 30) belonging to six bulls were used in the current study. The frozen bull semen samples were analysed in terms of proAKAP4 levels, sperm morphology and sperm movement parameters at hour 0 and hour 3 after thawing. The semen samples were divided into three groups according to the proAKAP4 levels: low concentration (<25 ng/10x106 spermatozoa), moderate concentration (25 to 39 ng/10x106 spermatozoa) and high concentration (≥40 ng/10x106 spermatozoa). A positive correlation was found between the proAKAP4 level and total motility (TM3), progressive motility (PM3), VSL3 and VCL3 values obtained after the third‐hour thermoresistance test (p < .05). There was a negative correlation between the percentage of sperm abnormal tail and the proAKAP4 level (p < .01). In addition, it was observed that the semen samples with proAKAP4 concentrations of 25 ng/106 spermatozoa and higher preserved the TM3 and PM3 motility characteristics. In conclusion, the proAKAP4 has the potential to become a biomarker protein to evaluate in the quality analysis of frozen‐thawed semen.

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Section: Methodsmentioning

confidence: 99%

Quality assessment of frozen bull semen with the precursor A‐kinase anchor protein 4 biomarker

Baştan

Akçay

2021

Andrologia

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“…As males mature, it can be difficult to predict the ability of spermatozoa to fertilize ova in vivo; that prediction is complicated by many factors including bull breed and sperm characteristics [23] [24]. Pregnancy rates have been associated with sperm hyperactivity [18], and sperm velocity [25]. Our prediction analyses found that velocity characteristics of fresh semen were the most informative predictors of sperm quality following cryopreservation; specifically, VAP was selected in half of the traits predicted.…”

Section: Discussionmentioning

confidence: 85%

Bovine Sperm Motility as Affected by Alpha Tocopherol and Ascorbic Acid during Storage

Page¹,

Rosenkrans²

2019

ARSci

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Our purpose was to determine the impact of ascorbic acid and α-tocopherol on bovine sperm motility following short-term storage or cryopreservation. Semen was collected from mature Angus bulls (n = 4). The experimental design was a randomized complete block, with bull serving as block, and treatments were structured as a 4 × 4 factorial with four concentrations of ascorbic acid (0, 5, 10, 20 mM) and four concentrations of α-tocopherol (0, 0.05, 0.5, 5 mM). Sperm motility characteristics were evaluated using computer assisted sperm analyses at 0, 4, and 8 h of incubation (39˚C) and post-cryopreservation. Initial sperm motility was (79.6% ± 1.6) and decreased to (6.1% ± 1.6%) after cryopreservation. Cryopreserved spermatozoa had lower (P < 0.05) post-thaw qualities when compared with fresh collections and for spermatozoa that had been incubated for 4 h at 39˚C. In contrast, most of the motility characteristics of spermatozoa that were incubated for 8 h at 39˚C were similar to those of post-thaw spermatozoa. Sperm motility, hyperactivity, and velocity characteristics were not affected (P > 0.1) by α-tocopherol. Addition of 20 mM ascorbic acid to storage media decreased (P < 0.05) sperm velocity traits, but the addition of 5 or 10 mM ascorbic acid did not alter sperm velocity. Sperm cell oxidation following cryopreservation/post-thaw was affected by an interaction (P < 0.05) between concentrations of ascorbic acid and α-tocopherol. Stepwise regression models predicted (P < 0.05) post-thaw motility and velocity characteristics of cryopreserved spermatozoa. Our results suggest that adding ascorbic acid and α-tocopherol was not beneficial for short-term storage of spermatozoa; however, our results were inconclusive with regards to inclusion of ascorbic acid and α-tocopherol in egg yolk-based cryopreservation media.

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“…It could lead to questioning as to whether chosen settings were extreme enough to detect any differences? Tested range values were selected to be consistent with the value variations generally observed in published works (Chaveiro et al, 2006;İnanç et al, 2018;Lenz et al, 2011). Some kinematic parameters (such as VSL) increased with the threshold value modifications, but this was not significant.…”

Section: Discussionmentioning

confidence: 87%

The effect of adjusting settings within a Computer-Assisted Sperm Analysis (CASA) system on bovine sperm motility and morphology results

O’Meara¹,

Henrotte

Kupisiewicz³

et al. 2022

Anim. Reprod.

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Semen motility is the most widely recognized semen quality parameter used by Artificial Insemination (AI) centers. With the increasing worldwide export of semen between AI centers there is an increasing need for standardized motility assessment methods. Computer-Assisted Sperm Analysis (CASA) technology is thought to provide an objective motility evaluation; however, results can still vary between laboratories. The aim of present study was to verify the impact of different setting values of the CASA IVOS II on motility, concentration, and morphology of bovine semen samples frozen in an extender with or without egg yolk and then decide on optimal settings for a further validation step across AI centers. Semen straws from 30 different bulls were analyzed using IVOS II with twelve modified settings. No significant changes were observed in semen concentration, percentage of motile sperm or kinetic results for either extender type. However, increasing settings for both STR and VAP progressive (%) from Low, Medium, and High cut-off values significantly (p<0.05) reduced the percentage of detected progressive spermatozoa, in egg yolk extender from 49.5±15.2, 37.2±11.9 to 11.9±5.3%, and in clear extender from 51.9±9.1, 35.8±7.3 to 10.0±2.4%, respectively. In clear extender only, the modification of droplet proximal head length significantly affected the detection of normal sperm percentages (88.0± 4.7 to 95.0±0.6 and 96.0±0.6%) and of the percentage of detected proximal droplets (12.2±4.7, 2.5±2.7 to 0.6±0.2%) for Low, Medium and High values respectively (p<0.05). The identification of sensitivity within the CASA system to changes in set parameters then led to the determination of an optimal IVOS II setting. The existing variability among centers for these phenotypes was reduced when the standardized settings were applied across different CASA units. The results clearly show the importance of applied settings for the final CASA results and emphasize the need for standardized settings to obtain comparable data.

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The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen

Cited by 17 publications

References 25 publications

Quality assessment of frozen bull semen with the precursor A‐kinase anchor protein 4 biomarker

Quality assessment of frozen bull semen with the precursor A‐kinase anchor protein 4 biomarker

Bovine Sperm Motility as Affected by Alpha Tocopherol and Ascorbic Acid during Storage

The effect of adjusting settings within a Computer-Assisted Sperm Analysis (CASA) system on bovine sperm motility and morphology results

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