The combination of Corynebacterium pseudotuberculosis recombinant proteins rPLD, rCP01850 and rCP09720 for improved detection of caseous lymphadenitis in sheep by ELISA

Silva, Mara Thais de Oliveira; Bezerra, Francisco Silvestre Brilhante; Pinho, Rodrigo Barros de; Ferreira, Caroline de Santana; Vivas, Wanessa Lordêlo Pedreira; Portela, Ricardo Wagner; Azevedo, Vasco; Borsuk, Sibele

doi:10.1099/jmm.0.001096

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…Currently, there are no reliable tests available to diagnose all CL cases. ELISA, using several antigenic preparations, has already been tested (Sutherland et al 1987;Menzies et al 1994;Sting et al 1998;Carminati et al 2003); however, few formulations use recombinant proteins (Menzies et al 1994; Rezende et al 2016; Barral et al 2019;Silva et al 2019). This strategy could signi cantly increase sensitivity and speci city due to the use of a single puri ed antigen (Rezende et al 2016).…”

Section: Introductionmentioning

confidence: 99%

rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

Farias¹,

Filho

Marchioro

et al. 2020

Preprint

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Caseous lymphadenitis (CL) is a chronic infectious disease that affects sheep and goats. Many serological tests have been developed to detect the disease; one of the most widely used is the enzyme-linked immunosorbent assay (ELISA), due to its advantages. ELISA formulations using recombinant proteins can exhibit significant sensitivity and specificity when using a single purified antigen. DTxR, Trx, TrxR, LexA, SodC, SpaC, NanH, and PknG recombinant proteins can be considered target proteins for ELISA development. Therefore, the objectives of this study were to evaluate the antigenic reactivity of Corynebacterium pseudotuberculosis recombinant proteins in goat and sheep serum. Of eight proteins evaluated, rSodC was selected for validation assays with small ruminant serum samples from the semiarid region of the state of Bahia, Brazil. Validation assays with goat serum samples showed that rSodC presented sensitivity and specificity of 96% and 94%, respectively. Validation assays with sheep serum showed that recombinant SodC exhibited sensitivity and specificity of 95% and 98%, respectively. Analysis of 756 field serum samples showed that rSodC identified 95 positive samples (23%) in goats and 75 positive samples (21%) in sheep. The ELISA with recombinant SodC protein developed in this study discriminated positive and negative serum samples with high levels of sensitivity and specificity. This formulation is promising for epidemiological surveys and CL control programs.

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Section: Introductionmentioning

confidence: 99%

rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

Farias¹,

Filho

Marchioro

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Currently, there are no reliable tests available to diagnose all CL cases. ELISA, using several antigenic preparations, has already been tested (Sutherland et al 1987 ; Menzies et al 1994 ; Sting et al 1998 ; Carminati et al 2003 ); however, few formulations use recombinant proteins (Menzies et al 1994 ; Rezende et al 2016 ; Barral et al 2019 ; Silva et al 2019 ). This strategy could significantly increase sensitivity and specificity due to the use of a single purified antigen (Rezende et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

et al. 2020

View full text Add to dashboard Cite

Caseous lymphadenitis (CL) is a chronic infectious disease that affects sheep and goats. Many serological tests have been developed to detect the disease; one of the most widely used is the enzyme-linked immunosorbent assay (ELISA), due to its advantages, which include acceptable cost-effectiveness, applicability, sensitivity and specificity. ELISA formulations using recombinant proteins can exhibit significant sensitivity and specificity when using a single purified antigen. DTxR, Trx, TrxR, LexA, SodC, SpaC, NanH, and PknG recombinant proteins can be considered target proteins for ELISA development due to its extracellular or on the cell surface location, which allows a better recognition by the immune system. Therefore, the objectives of this study were to evaluate the antigenic reactivity of Corynebacterium pseudotuberculosis recombinant proteins in goat and sheep serum. Of eight proteins evaluated, rSodC was selected for validation assays with small ruminant serum samples from the semiarid region of the state of Bahia, Brazil. Validation assays with goat serum samples showed that ELISA-rSodC presented sensitivity and specificity of 96% and 94%, respectively. Validation assays with sheep serum showed that ELISA-rSodC exhibited sensitivity and specificity of 95% and 98%, respectively. Analysis of 756 field serum samples showed that rSodC identified 95 positive samples (23%) in goats and 75 positive samples (21%) in sheep. The ELISA with recombinant SodC protein developed in this study discriminated positive and negative serum samples with high levels of sensitivity and specificity. This formulation is promising for epidemiological surveys and CL control programs. Trial registration AEC No 4958051018. 12/18/2018, retrospectively registered

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Assessment of rSodC, rPknG, rNanH, and rSpaC as Antigens for Diagnostic Tools Against Caseous Lymphadenitis

et al. 2022

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The combination of Corynebacterium pseudotuberculosis recombinant proteins rPLD, rCP01850 and rCP09720 for improved detection of caseous lymphadenitis in sheep by ELISA

Cited by 4 publications

References 37 publications

rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

Assessment of rSodC, rPknG, rNanH, and rSpaC as Antigens for Diagnostic Tools Against Caseous Lymphadenitis

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