The Combination of Duvelisib, a PI3K-δ,γ Inhibitor, and Romidepsin Is Highly Active in Relapsed/Refractory Peripheral T-Cell Lymphoma with Low Rates of Transaminitis: Results of Parallel Multicenter, Phase 1 Combination Studies with Expansion Cohorts

Abstract

Introduction: Most therapies for relapsed/refractory (rel/ref) T-cell lymphomas (TCLs) induce responses in about 25%-30% of pts. Phosphoinositide-3-kinase (PI3K) delta has a role in survival and proliferation of malignant T cells as well as T-cell receptor and cytokine signaling in nonmalignant T cells. Inhibition of PI3K gamma can reprogram macrophages and promote tumor phagocytosis. A phase I study of the PI3K-δ/γ inhibitor duvelisib (D) in pts with rel/ref TCLs showed promising activity, but high rates of grade (Gr) 3/4 ALT elevation at the MTD of 75 mg BID (Horwitz et al, Blood 2018). Based on in vitro evidence of synergy, we initiated a phase I/II study of D combined with either romidepsin (R) or bortezomib (B). Methods: Pts were enrolled into parallel phase I dose escalation arms utilizing a 3+3 design to define the maximum tolerated dose (MTD) of D combined with either R (Arm A) or B (Arm B) in cycle 1. D was dosed at 25 mg, 50mg, or 75 mg BID on days 1-28 with either R (10 mg/m2 on days 1, 8, & 15) or B (1 mg/m2 on days 1, 4, 8, & 11) each on 28-day cycles. Once the MTD was established with each combination, preplanned expansion cohorts were enrolled to further characterize safety and describe subtype-specific efficacy (PTCL and CTCL). Based upon promising safety and efficacy, a total of 39 pts were treated on Arm A (33 at the MTD, D 75 mg BID + R 10 mg/m2). All pts received prophylaxis against Varicella and Pneumocystis. Response assessments were performed q2 cycles for 6 months and then q3 cycles. To assess biomarkers of response and resistance to single-agent D, 10 pts in Arm A (75 mg BID) and 10 pts in Arm B (25 mg BID) were each treated with 1 cycle of D alone, with pretreatment and on-treatment biopsies. Pts who did not achieve CR on D alone at the end of cycle 1 proceeded to combination therapy. Results: The MTD was not reached in Arm A (R+D); thus, dose level 3(DL3); (D 75 mg BID + R 10 mg/m2 days 1, 8, & 15) was deemed the MTD and used for expansion. In Arm B there were no cycle 1 DLTs. However, Gr 3 elevations of ALT or AST following cycle 2 were observed in 3 pts at DL2 (D 50mg BID) and 2 pts at DL3 (D 75mg BID) leading to DL1 (D 25mg BID + B 1mg/m2 days 1, 4, 8, & 11) being accepted as MTD for expansion. Of Arm A pts at the MTD, 21/32 (65%) had adverse events (AEs) ≥Gr 3, possibly related to study drug. Events occurring in ≥10% of pts included: increased ALT/AST (n=5, 15%), neutropenia (n=6, 18%), and hyponatremia (n=4, 12%). Three pts had ≥Gr 3 diarrhea. There were no Gr 5 AEs related to protocol therapy. Strikingly, 4 of 5 pts with elevated transaminases (ALT [4], AST [1]) on combination began on the D-only Lead-In Arm at 75 mg BID. In contrast, only 1 of 22 (4%) pts receiving combination R+D in cycle 1 had Gr 3-4 transaminitis (p=.0242). Of the pts with Gr 3-4 diarrhea, 2 of 3 were on Lead-In (p=.0793). In Arm A, 35/39 pts were evaluable for response. Overall response rate (ORR) across all DLs was 51% (18/35) and CR rate (CR) was 17% (6/35). PTCL, ORR and CR rates were 55% (12/22) and 27% (6/22) respectively. Among CTCL, ORR was 46% (6/13), no CR. Reponses by histology are detailed in Table 1. Of these responders, 3 proceeded to allogeneic stem cell transplantation (allo SCT) with curative intent. Of note, 4 pts were not evaluable for response, described in Table 1. Median PFS for Arm A (all DL) was 8.8 m (PTCL) and 5.4 m (CTCL). Median follow up was 5.8 m, and median duration of response was 9.1 m. Of Arm B pts at the MTD, 10/22 (45%) had AEs ≥Gr 3, possibly related to study drug, of these, only neutropenia (n=4, 18%) occurred in ≥10% of pts at the MTD. There was 1 Gr 5 event, Stevens-Johnson syndrome, possibly related to protocol therapy. In Arm B, the ORR across all DLs was 32% (9/28), the CR rate was 11% (3/28). ORR in PTCL was 36% (5/14), 21% (3/14) achieved CR. ORR in CTCL was 28% (4/14), no CR. Responses by histology are detailed in Table 2. Of these responders, 1 proceeded to allo SCT with curative intent. Median PFS for Arm B (all DL) was 3.5 m (PTCL) and 4.6 m (CTCL). Median follow up was 7.2 m, and median duration of response was 9.3 m. Conclusion: Duvelisib in combination with romidepsin is highly active in pts with PTCL with tolerable side effects. Duvelisib can be safely combined with romidepsin at a 3-fold higher dose than with bortezomib (75 mg BID vs 25 mg BID) with much lower rate of Gr 3-4 transaminitis than single-agent duvelisib at the same dose. The high response rates and safety of Arm A (Duvelisib + Romidepsin) in PTCL appears to be a potential therapeutic advance and warrants further evaluation in a larger study. Disclosures Horwitz: Portola: Consultancy; Innate Pharma: Consultancy; Forty Seven: Consultancy, Research Funding; Kyowa-Hakka-Kirin: Consultancy, Research Funding; ADC Therapeutics: Consultancy, Research Funding; Seattle Genetics: Consultancy, Research Funding; Millennium/Takeda: Consultancy, Research Funding; Mundipharma: Consultancy; Corvus: Consultancy; Celgene: Consultancy, Research Funding; Infinity/Verastem: Consultancy, Research Funding; Aileron Therapeutics: Consultancy, Research Funding; Spectrum: Research Funding; Trillium: Consultancy. Moskowitz:Takeda: Honoraria; Incyte: Research Funding; Seattle Genetics: Consultancy, Honoraria, Research Funding; Bristol Myers-Squibb: Consultancy, Research Funding; Merck: Research Funding; ADC Therapeutics: Research Funding. Jacobsen:Seattle Genetics: Consultancy; Merck: Consultancy. Mehta-Shah:Spectrum: Consultancy; Bristol-Myers Squibb: Research Funding; Celgene: Research Funding; Genetech: Research Funding; Verastem: Research Funding. Khodadoust:Innate Pharma: Research Funding. Fisher:Seattle Genetics Inc.: Membership on an entity's Board of Directors or advisory committees; Genetech: Membership on an entity's Board of Directors or advisory committees; Pharmacyclics: Membership on an entity's Board of Directors or advisory committees. Kim:Medivir: Membership on an entity's Board of Directors or advisory committees; Portola: Research Funding; miRagen: Research Funding; Kyowa-Kirin-Pharma: Membership on an entity's Board of Directors or advisory committees, Research Funding; Neumedicine: Consultancy, Research Funding; Seattle Genetics: Membership on an entity's Board of Directors or advisory committees; Innate Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Tetralogic: Research Funding; Merck: Research Funding; Galderma: Research Funding; Takeda: Membership on an entity's Board of Directors or advisory committees, Research Funding; Horizon Pharma: Consultancy, Research Funding; Forty Seven Inc: Research Funding; Soligenix: Research Funding; Eisai: Membership on an entity's Board of Directors or advisory committees, Research Funding. Weinstock:Travera: Equity Ownership; Astra Zeneca, JAX, Samumed, Regeneron, Sun Pharma, Prescient: Patents & Royalties; Novartis, Dragonfly, Travera, DxTerity, Travera: Consultancy; Novartis, Astra Zeneca, Abbvie, Aileron, Surface Oncology, Daiichi Sankyo: Research Funding; Novartis: Consultancy, Research Funding; Genentech/Roche, Monsanto: Consultancy.

Updates in the Treatment of Peripheral T-Cell Lymphomas

“…Grade 3 or higher adverse events were seen in 65% of patients. 64 These results suggest that romidepsin + duvelisib could be a potential therapeutic strategy to be evaluated in larger studies.…”

Biomarker-driven management strategies for peripheral T cell lymphoma

“…Duvelasib is also being studied in combination with romidepsin and bortezomib in a parallel phase I study in patients with PTCL and CTCL. Duvelasib is given at 75 mg twice daily on days 1-28 on 28-day cycles with either romidepsin 10 mg/m 2 on days 1, 8, and 15 (arm A) or with bortezomib 1 mg/m 2 on days 1, 4, 8, and 11 (arm B) [56,57]. These combinations show activity with ORR and CR rates of 55% and 27% for patients with PTCL treated on arm A, and 36% and 21%, respectively, in patients treated on arm B.…”

Treatment approaches in relapsed or refractory peripheral T-cell lymphomas

“…Horwitz and colleagues described a small, parallel phase I study of RR T-cell lymphoma patients, in which there were 21 evaluable PTCL patients who received romidepsin and duvelisib (ORR 55% and CR 27%) or bortezomib with duvelisib (ORR 36% and CR 21%). Transaminitis limited the tolerability of bortezomib with duvelisib, but, given the tolerability and activity of romidepsin and duvelisib, an expansion of that cohort is planned 60 . Other combinations of novel treatments that are in the early stages of evaluation include pralatrexate with bortezomib 61 and romidepsin with pembrolizumab 62 .…”