The combination of ınterleukin-10 −1082 and tumor necrosis factor α −308 or ınterleukin-6 −174 genes polymorphisms suggests an association with susceptibility to Hashimoto's thyroiditis

Baki, Merve; Akman, Fevziye E.; Vural, Pervin; Doğru‐Abbasoğlu, Semra; Özderya, Ayşenur; Karadağ, Berrin; Uysal, Müjdat

doi:10.1016/j.intimp.2012.02.003

Cited by 18 publications

(10 citation statements)

References 36 publications

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“…The comparative analysis of the IL-10 ¡1082 A/G allele frequencies of the patients and the control group revealed a significantly higher frequency of the homozygous GG genotype in HT patients. Baki et al [32] as well as Hunt et al [28] did not report differences in the G allele frequency in HT patients compared to controls. Inoue et al [33] studied the functional ¡592 A/C polymorphism in the gene encoding IL-10 and reported that the CC genotype, which is associated with higher production of IL-10, was more frequent in patients with severe HT than in those with mild HT.…”

Section: Resultsmentioning

confidence: 91%

Impact of IL-10 and IL-12B single nucleotide polymorphisms on circulating cytokine level in development of Hashimoto's thyroiditis

Gerenova

Manolova

Stanilova

2016

Biotechnology & Biotechnological Equipment

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Hashimoto's thyroiditis (HT) is an organ-specific, mainly Th1 autoimmune disorder. New evidence has accumulated for involvement of Treg-produced cytokines. Interleukin (IL)-12 and IL-10 are immunoregulatory cytokines with antagonistic effect on Th differentiation. This study was designed to investigate the correlation of circulating IL-10 and IL-12p40 with their genotypes in 124 HT patients in different stages of disease. The IL-10 and IL-12p40 circulating level in the serum of HT patients and healthy controls was determined by enzyme-linked immunosorbent assay. Genotyping for the 3'UTR A/C IL12B polymorphism was performed using restriction fragment length polymorphismÀpolymerase chain reaction and genotyping for ¡1082 A/G by amplification refractory mutation system (ARMS)-PCR. The results showed significantly enhanced IL-12p40 serum quantity both in euthyroid and hypothyroid stages compared to controls and insignificantly decreased level after levothyroxine treatment. Regarding the 3'UTR A/C IL12B polymorphism, a significantly higher frequency of the AA genotype was observed in HT patients than in healthy individuals. The serum IL-10 level was significantly increased in hypothyroid HT patients compared to controls and euthyroid patients. Stratification based on the ¡1082 A/G polymorphism revealed a significantly enhanced level of circulating IL-10 in hypothyroid patients with a GG genotype compared to AA and AG genotypes. There were also significant differences between the circulating IL-10 quantity in hypo-and euthyroid patients with GG genotypes. In conclusion, the IL-12p40 and IL-10 serum level in HT patients was dependent on the genotype and HT stages, suggesting that enhanced IL-10 serum level in combination with enhanced IL-12p40 is associated with hypothyroidism in HT development.

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Section: Resultsmentioning

confidence: 91%

Impact of IL-10 and IL-12B single nucleotide polymorphisms on circulating cytokine level in development of Hashimoto's thyroiditis

Gerenova

Manolova

Stanilova

2016

Biotechnology & Biotechnological Equipment

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“…Three studies were excluded because they contained other IL10 polymorphisms, data duplication, or did not contain genotype data. Ten studies met our inclusion criteria . Two of these studies contained data on two different groups .…”

Section: Resultsmentioning

confidence: 99%

Association of Interleukin 10 Gene Polymorphisms with Autoimmune Thyroid Disease: Meta‐Analysis

et al. 2016

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The aim of this study was to perform a meta-analysis of eligible studies and to derive a precise estimate of the association between interleukin 10 (IL10) polymorphisms and susceptibility to autoimmune thyroid disease (AITD). Meta-analyses were conducted on the associations between AITD and the -1082 G/A (rs1800896), -819 C/T (rs1800871) and -592 C/A (rs1800872) polymorphisms in IL10, and the haplotype of these polymorphisms and AITD. A total of 2903 AITD patients and 3060 controls in 10 eligible studies were included in the meta-analysis. This meta-analysis showed significant associations between IL10 at the -1082 G allele and overall AITD (OR: 1.44, 95% CI 1.13-1.82, P = 0.003), but no association between the IL10 -592 C allele and the -819 C allele and AITD. Subgroup studies demonstrated significant associations between the -1082 G allele and susceptibility to Graves' disease. Ethnicity-specific meta-analysis revealed significant associations between the -1082 G allele and AITD susceptibility in Asian populations; however, in Middle Eastern populations, no association was evident. Meta-analysis of the IL10 haplotype revealed an association between the ATA haplotype and AITD (OR: 1.17, 95% CI 1.00-1.36, P = 0.04). Meta-analysis demonstrates that the IL10 polymorphisms are associated with susceptibility to AITD.

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“…The obtained results in our study for −1082 IL‐10 variant allele frequency (0.39) in healthy controls were similar to previously reported data for Bulgarian population and close to those reported in other Caucasian‐type cohorts in Turkey (0.38), Italy (0.34) and Spain (0.38), but lower than that found by Depboylu et al . (0.45) in a German population (Depboylu et al ., ; Castro‐Santos et al ., ; Scarpelli et al ., ; Miteva & Stanilova, ; Baki et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…The polymorphism at position −1082 in the promoter region of the IL‐10 gene has been studied by Baki and colleagues, who did not find notable risk for HT afflicted by IL‐10 −1082 polymorphism alone (Baki et al ., ). Authors reported no statistically significant difference in G allele frequency in patients with HT compared to controls.…”

Section: Discussionmentioning

confidence: 97%

IL‐12B and IL‐10 gene polymorphisms in the development of Hashimoto's thyroiditis

Gerenova

Stanilova

2016

Int J Immunogenetics

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Functional genetic polymorphisms that altered gene expression of cytokines are candidate genetic factors that could modulate the development and progression of Hashimoto's thyroiditis (HT). IL-12B gene encoded the IL-12p40 subunit, which is included in the pro-inflammatory heterodimeric cytokines IL-12p70 and IL-23. IL-10 is an important Treg cytokine suppressing inflammatory cytokine production and autoimmunity. This study was designed to compare -1082A/GIL-10 and +1188A/C3'UTRIL-12B genotype distribution in 130 patients with HT to a group of 157 healthy controls in attempts to determine an association with HT development. Genotyping for the 3'UTRA/C IL-12B polymorphism was performed using RFLP-PCR and genotyping for -1082A/G IL-10 by ARMS-PCR assay. Patients with HT were divided into euthyroid and hypothyroid stages. There were no significant differences in the genotype and allele frequencies of the IL-12B polymorphism between patients with HT and controls. We observed higher euthyroid HT risk for individuals with CC genotype, unlike to develop hypothyroidism with OR = 1.68. Regarding the polymorphism rs1800896, it was shown the significantly higher frequency of homozygous genotype GG in cases vs controls (OR = 2.19; P = 0.024). Moreover, the combination of genotype AA of 3'UTRIL-12B with GG of -1082IL-10 was associated with a threefold increasing risk (OR = 3.188; P = 0.022) of developing HT compared to individuals with the presence of 3'UTR allele C (AC+CC) simultaneously with AA genotype of -1082IL-10. Our data raise the possibility that the combined effect of polymorphisms from proinflammatory and anti-inflammatory cytokines may be more decisive to HT development.

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The combination of ınterleukin-10 −1082 and tumor necrosis factor α −308 or ınterleukin-6 −174 genes polymorphisms suggests an association with susceptibility to Hashimoto's thyroiditis

Cited by 18 publications

References 36 publications

Impact of IL-10 and IL-12B single nucleotide polymorphisms on circulating cytokine level in development of Hashimoto's thyroiditis

Impact of IL-10 and IL-12B single nucleotide polymorphisms on circulating cytokine level in development of Hashimoto's thyroiditis

Association of Interleukin 10 Gene Polymorphisms with Autoimmune Thyroid Disease: Meta‐Analysis

IL‐12B and IL‐10 gene polymorphisms in the development of Hashimoto's thyroiditis

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