2020
DOI: 10.1101/2020.09.02.280404
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The Combination of Morphology and Surface Chemistry Defines the Biological Identity of Nanocarriers in Human Blood

Abstract: Following intravenous administration, an adsorbed corona of blood proteins immediately forms on the surfaces of nanocarriers to confer a distinct biological identity that dictates interactions with the immune system. While the nanocarrier surface chemistry has long been the focus of protein corona formation, the influence of the nanocarrier structure has remained unclear despite well-documented influences on biodistribution, clearance and inflammation. Here, we present design rules for the combined engineering… Show more

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Cited by 2 publications
(2 citation statements)
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“…We therefore investigated an even earlier timepoint of 2 h, which would both be relevant for these early clearance events as well as allow formation of a reasonably stable albumin-rich protein corona to minimize confounding effects arising from the potential dynamic exchange of albumin at later timepoints. Furthermore, we employed label-free quantitative mass spectrometry (MS) to demonstrate that serum albumin is a major constituent of the Phos PS, OH PS, and MeO PS protein corona formed in pooled human plasma at 2 h and 24 h timepoints in a separate study 49 . Consistent with these findings, serum albumin was also a major constituent of each protein corona formed in fetal bovine serum (FBS), which is indicated by the~67 kDa band observed on silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels 50 (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
“…We therefore investigated an even earlier timepoint of 2 h, which would both be relevant for these early clearance events as well as allow formation of a reasonably stable albumin-rich protein corona to minimize confounding effects arising from the potential dynamic exchange of albumin at later timepoints. Furthermore, we employed label-free quantitative mass spectrometry (MS) to demonstrate that serum albumin is a major constituent of the Phos PS, OH PS, and MeO PS protein corona formed in pooled human plasma at 2 h and 24 h timepoints in a separate study 49 . Consistent with these findings, serum albumin was also a major constituent of each protein corona formed in fetal bovine serum (FBS), which is indicated by the~67 kDa band observed on silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels 50 (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
“…To enhance the therapeutic potential of CQ drug delivery, future work should include investigating other nanocarrier morphologies that can target other IFN-producing cell types such as myeloid cells. The PEG-b-PPS platform is ideal for these studies because by changing the hydrophilic weight fractions of the polymer, they can be self-assembled easily to form diverse morphologies such as spherical (i.e., micelles and polymersomes) (32) and cubic (i.e., bicontinuous cubic nanospheres) (75) nanostructures with distinct cellular biodistribution profiles (76). Overall, this study illustrates the therapeutic potential of drug delivery of CQ for targeting SLE-relevant pathways, immune cells, and organ sites.…”
Section: Discussionmentioning
confidence: 99%