The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells

Eskandari, Neda; Moghaddam, Meysam Hassani; Atlasi, Mohammad Ali; Mahabadi, Javad Amini; Taherian, Aliakbar; Nikzad, Hossein

doi:10.1016/j.biologicals.2018.10.001

Cited by 11 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spermatogonium and spermatocyte development require RA signalling, while spermatozoa only use endogenous metabolite concentrations. [24][25][26][27][28] The requirement for retinoids in sperm development, and their ability to restore male fertility when topically administered, highlights the importance of these metabolites during spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

A systematic review of retinoic acid in the journey of spermatogonium to spermatozoa: From basic to clinical application

2022

View full text Add to dashboard Cite

Background: Retinoic acid plays an essential role in testicular development and functions, especially spermatogenesis. We have reviewed the role of retinoic acid from basic (molecular) to clinical application. Methods: A search was conducted in the online database including PubMed, Google Scholar, and Scopus for English studies published in the last eight years about this issue. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines in assessing the studies we are going to investigate. Results: Studies indicated that retinoic acid plays an essential role during pluripotent stem cell migration and lineage commitment, cell differentiation, apoptosis, stem cell number regulation, and maturation arrest in spermatogenic cells. Retinoic acid can also affect related protein expression and signaling pathways at different stages of spermatogenesis. Four studies have applied retinoic acid to humans, all of them in the single-arm observational study. The results look promising but need further research with more controlled study methods, randomization, and large samples. Conclusions: This current systematic review emphasizes a novel retinoic acid mechanism that has not been well described in the literature previously on its functions during the first seven days of spermatogenesis, leading to new directions or explanations of male infertility cause and treatments as a part of reproductive health care.

show abstract

Section: Discussionmentioning

confidence: 99%

A systematic review of retinoic acid in the journey of spermatogonium to spermatozoa: From basic to clinical application

2022

View full text Add to dashboard Cite

show abstract

“…RA is a multipurpose factor that controls the meiotic entrance of PGCs in the genital cords by controlling the Cyp26b1 enzyme expression (15). RA activity has also been reported in the ESCs resulting in the increased gene expression levels of several GC genes including FRAGILIS and STRA8 (41). Activity of RA is exerted inside the nucleus during gametogenesis through their binding to the RA receptors (RAR) in several cell types including Sertoli cells (RARα), round spermatids (RARβ), and type A spermatogonia (RARγ) (42).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of DAZL, STRA8, and BOULE Genes and Treatment With BMP4 or Retinoic Acid Modulate the Expression of MSC Overexpressing Germ Cell Genes

et al. 2021

View full text Add to dashboard Cite

In vitro gamete derivation from stem cells has potential applications in animal reproduction as an alternative method for the dissemination of elite animal genetics, production of transgenic animals, and conservation of endangered species. Mesenchymal stem cells (MSCs) may be suitable candidates for in vitro gamete derivation considering their differentiative capacity and their potential for cell therapy. Due to its relevance in gametogenesis, it has been reported that retinoic acid (RA) and bone morphogenetic protein (BMP) 4 are able to upregulate the expression of specific markers associated to the early stages of germ cell (GCs) differentiation in bovine fetal MSCs (bfMSCs). In the present study, we used polycistronic vectors containing combinations of GC genes DAZL, STRA8, and BOULE followed by exposure to BMP4 or RA to induce GC differentiation of bovine fetal adipose tissue-derived MSC (AT-MSCs). Cells samples at Day 14 were analyzed according to the expression of pluripotent genes NANOG and OCT4 and GC genes DAZL, STRA8, BOULE, PIWI, c-KIT, and FRAGILIS using Q-PCR. Fetal and adult testis and AT-MSCs samples were also analyzed for the expression of DAZL, STRA8, and NANOG using immunofluorescence. Increased gene expression levels in the adult testis and cell-specific distribution of DAZL, STRA8, and NANOG in the fetal testis suggest that these markers are important components of the regulatory network that control the in vivo differentiation of bovine GCs. Overexpression of DAZL and STRA8 in bi-cistronic and DAZL, STRA8, and BOULE in tri-cistronic vectors resulted in the upregulation of OCT4, NANOG, and PIWIL2 in bovine fetal AT-MSCs. While BMP4 repressed NANOG expression, this treatment increased DAZL and c-KIT and activated FRAGILIS expression in bovine fetal AT-MSCs. Treatment with RA for 14 days increased the expression of DAZL and FRAGILIS and maintained the mRNA levels of STRA8 in bovine fetal AT-MSCs transfected with bi-cistronic and tri-cistronic vectors. Moreover, RA treatment repressed the expression of OCT4 and NANOG in these cells. Thus, overexpression of DAZL, STRA8, and BOULE induced the upregulation of the pluripotent markers and PIWIL2 in transfected bovine fetal AT-MSCs. The partial activation of GC gene expression by BMP4 and RA suggests that both factors possess common targets but induce different gene expression effects during GC differentiation in overexpressing bovine fetal AT-MSCs.

show abstract

“…no. M4287; Eskandari et al, ; West, Mumaw, Mumaw, Gallegos‐Cardenas, Young, & Stice, ; Wongtrakoongate, Jones, Jones, Gokhale, & Andrews, ) in cell culture flask 25 cm 2 (Orange Scientific). Mouse iPSCs medium contained DMEM/F12 culture medium complemented with mmol/L L‐glutamine (Gibco, cat.…”

Section: Methodsmentioning

confidence: 99%

“…no. E2758‐1G; Eskandari et al, ; Hong, Zhang, Zhang, Sultana, Yu, & Wei, ) and 2 μmol retinoic acid (RA; Sigma, cat. no.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Retinoic acid and 17β‐estradiol improve male germ cell differentiation from mouse‐induced pluripotent stem cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

This research aimed to explore the impacts of retinoic acid (RA)/17β-estradiol (E) induction and embryoid body formation to enhance differentiation of mouse-induced pluripotent stem cells (miPSCs) into male germ cells in vitro. Flow cytometry and qPCR were conducted to describe miPSCs differentiation process. Various temporal expression profiles of germ cell-related genes were traced. Stra8 gene expression increased in the RA group on the 4th day compared to other groups. The RA group experienced a more significant increase than E group. The expression of Sycp3 increased in RA + E group on 4th day compared with other groups. Expression of AKAP3 enhanced in the RA + E group than other groups on day 4. Moreover, miPSCs showed that this gene expression in the RA + E group was increased in comparison to RA and E groups on day 7. AKAP3 gene expression on day 7 of miPSCs decreased in RA and E groups. Flow cytometry data indicated that 3%-8% of the cells in sub-G1 stage were haploid after RA and E induction compared to other groups on day 4. This study showed that miPSCs possess the power for differentiating into male germ cells in vitro via formation of embryoid body by RA with/or E induction.

show abstract

The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells

Cited by 11 publications

References 40 publications

A systematic review of retinoic acid in the journey of spermatogonium to spermatozoa: From basic to clinical application

A systematic review of retinoic acid in the journey of spermatogonium to spermatozoa: From basic to clinical application

Overexpression of DAZL, STRA8, and BOULE Genes and Treatment With BMP4 or Retinoic Acid Modulate the Expression of MSC Overexpressing Germ Cell Genes

Retinoic acid and 17β‐estradiol improve male germ cell differentiation from mouse‐induced pluripotent stem cells

Contact Info

Product

Resources

About