The combination of transcriptome and metabolome reveals the molecular mechanism by which topping and salicylic acid treatment affect the synthesis of alkaloids in
            <i>Nicotiana tabacum</i>
            L.

Zhang, Mengyue; Zhao, Yuanyuan; Yang, Chi; Shi, Hongzhi

doi:10.1080/26895293.2022.2025915

Cited by 5 publications

(7 citation statements)

References 34 publications

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“…contents, which is broadly consistent with the findings of previous studies (Zhao et al, 2018). Topping is an operation that suppresses the apical dominance of plants and promotes the rapid development of lateral branches (Zhang et al, 2022). In addition, topping can have the effects of delaying tobacco leaf senescence and promoting the accumulation of secondary metabolites in leaves (Zhao et al, 2018).…”

Section: Discussionsupporting

confidence: 90%

“…In the two treatment groups in which tobacco served as the scion, we found that with the exception of anabasine, compared with non‐topped plants, the leaves of topped plants had higher alkaloid contents, which is broadly consistent with the findings of previous studies (Zhao et al, 2018 ). Topping is an operation that suppresses the apical dominance of plants and promotes the rapid development of lateral branches (Zhang et al, 2022 ). In addition, topping can have the effects of delaying tobacco leaf senescence and promoting the accumulation of secondary metabolites in leaves (Zhao et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…For qualitative analysis, an ultrahigh‐performance liquid chromatograph coupled with a Q Exactive HF‐X mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) system equipped with an ACQUITYUPLCHSS T3 column (Waters Corporation, Milford, MA, USA) was used to perform the liquid chromatography–mass spectrometry analysis as previously described (Zhang et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Fresh tobacco leaves collected during the course of tobacco plant growth were initially cured at 105 C, and then dried at 60 C. The leaves of mature tobacco plants were cured after harvesting without initial fixation. Alkaloids were extracted from crushed dried leaf material using methyl tertbutyl ether under alkaline conditions (Zhang et al, 2022). A gas chromatograph incorporating a hydrogen flame ionization detector (Agilent 7890A; Agilent Technologies, USA) was used for quantitative analysis of alkaloid content, with n-hexadecane used as an internal standard (Guan et al, 2021).…”

Section: Determination Of Alkaloid Levelsmentioning

confidence: 99%

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Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacumL.)

2023

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In this study, we aimed to determine molecular mechanisms underlying the effects of topping and grafting in tobacco ( Nicotiana tabacum L.) by comparing the alkaloid contents and enrichment pathways of differentially expressed genes (DEGs) among plants subjected to different combinations of topping and grafting treatments. Plants of the tobacco variety “Zhongyan 100” and eggplant ( Solanum melongena L.) were grafted in four combinations as scions and rootstocks, respectively. The four treatment groups were tobacco with topping, tobacco without topping, topped tobacco grafted onto an eggplant rootstock, and non‐topped tobacco grafted onto an eggplant rootstock. Tobacco leaves were collected on the day of topping, at 7 days after topping, and after flue curing, the alkaloid contents of the collected leaves were determined. Leaves of plants subjected to the different treatments were collected for RNA sequencing and screened for DEGs, which were subsequently subjected to functional enrichment analyses. Analyses revealed reductions in the leaf alkaloid contents of tobacco subjected to combined topping and eggplant grafting. Gene annotation indicated that topping influences biological processes such as starch metabolism and stress response, whereas grafting affected the biosynthesis and metabolic pathways of secondary metabolites. Downregulated DEGs between non‐topped tobacco and eggplant‐grafted topped tobacco and between topped and non‐topped tobacco are mainly involved in inositol phosphate metabolic and biosynthetic processes. Downregulated DEGs between different grafting methods (eggplant‐grafted non‐topped tobacco vs. non‐topped tobacco and eggplant‐grafted topped tobacco vs. topped tobacco) are mainly involved in sesquiterpene synthase activity and photosynthesis. The findings of this study provide important insights into the molecular mechanisms underlying the effects of topping and grafting on tobacco plants.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Determination Of Alkaloid Levelsmentioning

confidence: 99%

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Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacumL.)

2023

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“…Similarly, many TFs were found for the biosynthesis of alkaloids, i.e., AP2/ERF, bHLH, MYB-related, WRKY, C2H2, GRAS, NAC, bZIP, C3H, B3, FAR1, GARP-G2-like, and HB-HD-ZIP from leaves and roots with mostly upregulating in the roots. The TFs, i.e., AP2/ERF, WRKY, C2H2, bHLH, and MYB-related, are major regulators for the biosynthesis of IQA, IA and TPPA alkaloids pathways in rice, cotton, tobacco, Fritillaria roylei, Catharanthus roseus, and V. californicum (Yamada et al, 2011;Yamada and Sato, 2013;Augustin et al, 2015;Van Moerkercke et al, 2015;Lu et al, 2022;Zhang et al, 2022). The first TF isolated for having a role in the biosynthesis process of alkaloids was AP2/ERF in response to Jasmonates (JA) (Yamada FIGURE 7 PCA and Correlation analysis of genes and metabolites detected by each differential group of samples from V. mengtzeanum (A) PCA of metabolome and (B) PCA of transcriptome data to visualize the differences between the sample groups of V. mengtzeanum in the transcripts and metabolites.…”

Section: Figurementioning

confidence: 99%

Transcriptome and metabolome profiling of the medicinal plant Veratrum mengtzeanum reveal key components of the alkaloid biosynthesis

Han

et al. 2023

Front. Genet.

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Veratrum mengtzeanum is the main ingredient for Chinese folk medicine known as “Pimacao” due to its unique alkaloids. A diverse class of plant-specific metabolites having key pharmacological activities. There are limited studies on alkaloid synthesis and its metabolic pathways in plants. To elucidate the alkaloid pathway and identify novel biosynthetic enzymes and compounds in V. mengtzeanum, transcriptome and metabolome profiling has been conducted in leaves and roots. The transcriptome of V. mengtzeanum leaves and roots yielded 190,161 unigenes, of which 33,942 genes expressed differentially (DEGs) in both tissues. Three enriched regulatory pathways (isoquinoline alkaloid biosynthesis, indole alkaloid biosynthesis and tropane, piperidine and pyridine alkaloid biosynthesis) and a considerable number of genes such as AED3-like, A4U43, 21 kDa protein-like, 3-O-glycotransferase 2-like, AtDIR19, MST4, CASP-like protein 1D1 were discovered in association with the biosynthesis of alkaloids in leaves and roots. Some transcription factor families, i.e., AP2/ERF, GRAS, NAC, bHLH, MYB-related, C3H, FARI, WRKY, HB-HD-ZIP, C2H2, and bZIP were also found to have a prominent role in regulating the synthesis of alkaloids and steroidal alkaloids in the leaves and roots of V. mengtzeanum. The metabolome analysis revealed 74 significantly accumulated metabolites, with 55 differentially accumulated in leaves compared to root tissues. Out of 74 metabolites, 18 alkaloids were highly accumulated in the roots. A novel alkaloid compound viz; 3-Vanilloylygadenine was discovered in root samples. Conjoint analysis of transcriptome and metabolome studies has also highlighted potential genes involved in regulation and transport of alkaloid compounds. Here, we have presented a comprehensive metabolic and transcriptome profiling of V. mengtzeanum tissues. In earlier reports, only the roots were reported as a rich source of alkaloid biosynthesis, but the current findings revealed both leaves and roots as significant manufacturing factories for alkaloid biosynthesis.

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Impact of Cold Stress on Leaf Structure, Photosynthesis, and Metabolites in Camellia weiningensis and C. oleifera Seedlings

Huang

Jiang

et al. 2022

Horticulturae

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Camellia weiningensis Y. K. Li. sp. nov. (CW) is an endemic oil-tea species in Guizhou province, distributed in the alpine karst area, which exhibits cold resistance and better economic characters than C. oleifera (CO). The mechanism of cold response in CW seedlings has not been studied in depth. Herein, we performed anatomical, physiological, and metabolic analyses to assess the impact of cold stress on leaf structure, photosynthesis, and metabolites in CW and CO seedlings. Anatomical analysis of leaves showed CW seedlings had greater leaf and palisade thicknesses, tissue structure tightness, and palisade-spongy tissue ratio to enhance chilling stress (4 °C) tolerance, but freezing stress (−4 °C) caused loosening of the leaf tissue structure in both CW and CO seedlings. Photosynthetic analysis showed a reduction in the chlorophyll (Chl) fluorescence (Fv/Fm) and photosynthetic parameters under freezing stress in both CW and CO seedlings. Cold stress increased the abscisic acid (ABA) contents in both the Camellia species, and CW exhibited the highest ABA content under −4 °C treatment. Additionally, the indole-3-acetic acid (IAA) content was also increased in CW in response to cold stress. An obviously distinct metabolite composition was observed for CW and CO under different temperatures, and significantly changed metabolites (SCMs) were enriched under freezing stress. Prenol lipids, organooxygen compounds, and fatty acyls were the main metabolites in the two Camellia species in response to cold stress. The top key SCMs, such as medicoside G, cynarasaponin F, yuccoside C, and methionyl-proline were downregulated under freezing stress in both CW and CO. The contents of some key metabolites associated with sugar metabolism, such as UDP-glucose, UDP-D-apiose, and fructose 6-phosphate, were higher in CW than in CO, which may contribute to enhancing the cold resistance in CW. Our findings are helpful in explaining how CW adapt to alpine karst cold environments, and will provide a reference for cold tolerance improvement and application of stress-resistant breeding of Camellia in alpine and cold areas.

show abstract

The combination of transcriptome and metabolome reveals the molecular mechanism by which topping and salicylic acid treatment affect the synthesis of alkaloids in Nicotiana tabacum L.

Cited by 5 publications

References 34 publications

Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacumL.)

Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Nicotiana tabacumL.)

Transcriptome and metabolome profiling of the medicinal plant Veratrum mengtzeanum reveal key components of the alkaloid biosynthesis

Impact of Cold Stress on Leaf Structure, Photosynthesis, and Metabolites in Camellia weiningensis and C. oleifera Seedlings

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