The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells

Maulucci, Giuseppe; Cohen, Ofir; Daniel, Bareket; Ferreri, Carla; Sasson, Shlomo

doi:10.3390/molecules24203742

Cited by 17 publications

(12 citation statements)

References 81 publications

(104 reference statements)

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“…Of note, family‐level Prevotellaceae , genus‐level Prevotella were highly associated with dystrophic mdx mice. Prevotella has been associated with either good or bad metabolic outcomes and, probably, both depend on the presence of metabolic and inflammatory alterations (Maulucci et al , 2019). Prevotella was more abundant in patients suffering from untreated rheumatoid arthritis than in healthy individuals with a reduction in the abundance of beneficial microbes.…”

Section: Discussionmentioning

confidence: 99%

Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin‐deficient mice

et al. 2022

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Duchenne muscular dystrophy (DMD) is a progressive severe muscle-wasting disease caused by mutations in DMD, encoding dystrophin, that leads to loss of muscle function with cardiac/respiratory failure and premature death. Since dystrophic muscles are sensed by infiltrating inflammatory cells and gut microbial communities can cause immune dysregulation and metabolic syndrome, we sought to investigate whether intestinal bacteria support the muscle immune response in mdx dystrophic murine model. We highlighted a strong correlation between DMD disease features and the relative abundance of Prevotella. Furthermore, the absence of gut microbes through the generation of mdx germfree animal model, as well as modulation of the microbial community structure by antibiotic treatment, influenced muscle immunity and fibrosis. Intestinal colonization of mdx mice with eubiotic microbiota was sufficient to reduce inflammation and improve muscle pathology and function. This work identifies a potential role for the gut microbiota in the pathogenesis of DMD.

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Section: Discussionmentioning

confidence: 99%

Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin‐deficient mice

et al. 2022

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“…Three reviews deal with confocal microscopy-based detection [ 31 ], mechanistic studies of methionine (Met) oxidation [ 32 ], and recent applications of chemiluminescence (CL) [ 33 ]. One of them focuses on confocal microscopy-based detection of profound alterations in the plasma membrane, membranes of insulin granules and lipid droplets in single beta cells under various nutritional load conditions.…”

Section: Brief Overview Of the Two Special Issuesmentioning

confidence: 99%

“…One of them focuses on confocal microscopy-based detection of profound alterations in the plasma membrane, membranes of insulin granules and lipid droplets in single beta cells under various nutritional load conditions. The combination of whole cell lipidomics analysis and single cell confocal imaging of fluidity and micropolarity provides insight into stress-induced lipid turnover in subcellular organelles of pancreatic beta cells [ 31 ].…”

Section: Brief Overview Of the Two Special Issuesmentioning

confidence: 99%

Biomimetic Radical Chemistry and Applications

Chatgilialoglu

2022

Molecules

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Some of the most interesting aspects of free radical chemistry that emerged in the last two decades are radical enzyme mechanisms, cell signaling cascades, antioxidant activities, and free radical-induced damage of biomolecules. In addition, identification of modified biomolecules opened the way for the evaluation of in vivo damage through biomarkers. When studying free radical-based chemical mechanisms, it is very important to establish biomimetic models, which allow the experiments to be performed in a simplified environment, but suitably designed to be in strict connection with cellular conditions. The 28 papers (11 reviews and 17 articles) published in the two Special Issues of Molecules on “Biomimetic Radical Chemistry and Applications (2019 and 2021)” show a remarkable range of research in this area. The biomimetic approach is presented with new insights and reviews of the current knowledge in the field of radical-based processes relevant to health, such as biomolecular damages and repair, signaling and biomarkers, biotechnological applications, and novel synthetic approaches.

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“…Other phospholipids, such as phosphatidylethanolamine, can also participate in the LCAT reaction ( Christiaens et al, 2000 ). Fatty acids in phospholipid membranes and cholesteryl esters from the plasma have a close correlation with the onset of insulin resistance and diabetes ( Maulucci et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Targeted and untargeted metabolomic approach for GDM diagnosis

Burzyńska-Pędziwiatr

Dudzik

Sansone

et al. 2023

Front. Mol. Biosci.

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Gestational diabetes mellitus (GDM) is a disorder which manifests itself for the first time during pregnancy and is mainly connected with glucose metabolism. It is also known that fatty acid profile changes in erythrocyte membranes and plasma could be associated with obesity and insulin resistance. These factors can lead to the development of diabetes. In the reported study, we applied the untargeted analysis of plasma in GDM against standard glucose-tolerant (NGT) women to identify the differences in metabolomic profiles between those groups. We found higher levels of 2-hydroxybutyric and 3-hydroxybutyric acids. Both secondary metabolites are associated with impaired glucose metabolism. However, they are products of different metabolic pathways. Additionally, we applied lipidomic profiling using gas chromatography to examine the fatty acid composition of cholesteryl esters in the plasma of GDM patients. Among the 14 measured fatty acids characterizing the representative plasma lipidomic cluster, myristic, oleic, arachidonic, and α-linoleic acids revealed statistically significant changes. Concentrations of both myristic acid, one of the saturated fatty acids (SFAs), and oleic acid, which belong to monounsaturated fatty acids (MUFAs), tend to decrease in GDM patients. In the case of polyunsaturated fatty acids (PUFAs), some of them tend to increase (e.g., arachidonic), and some of them tend to decrease (e.g., α-linolenic). Based on our results, we postulate the importance of hydroxybutyric acid derivatives, cholesteryl ester composition, and the oleic acid diminution in the pathophysiology of GDM. There are some evidence suggests that the oleic acid can have the protective role in diabetes onset. However, metabolic alterations that lead to the onset of GDM are complex; therefore, further studies are needed to confirm our observations.

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The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells

Cited by 17 publications

References 81 publications

Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin‐deficient mice

Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin‐deficient mice

Biomimetic Radical Chemistry and Applications

Targeted and untargeted metabolomic approach for GDM diagnosis

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