The Combinatorial Nature of Osmosensing in Fishes

Kültz, Dietmar

doi:10.1152/physiol.00014.2012

Cited by 84 publications

(66 citation statements)

References 189 publications

(210 reference statements)

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“…Among other tissues with high bmp2, 4 and 16 expression are the branchial arches, known to be important for processes such as osmoregulation and respiration, where BMP signaling is reported to be involved [76]. Bmp16 expression in this tissue may also suggest a role in cartilage formation and mineralization as already described for BMP2 and BMP4 [77].…”

Section: Discussionmentioning

confidence: 65%

Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives

Marques

Fernández

Viegas

et al. 2015

Cell. Mol. Life Sci.

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BMP2, BMP4 and BMP16 form a subfamily of bone morphogenetic proteins acting as pleiotropic growth factors during development and as a bone inducers during osteogenesis.BMP16 is the most recent member of this subfamily and basic data regarding protein structure and function, and spatio-temporal gene expression is still scarce. In this work, insights on BMP16 were provided through the comparative analysis of structural and functional data for zebrafish BMP2a, BMP2b, BMP4 and BMP16 genes and proteins, determined from threedimensional models, patterns of gene expression during development and in adult tissues, regulation by retinoic acid and capacity to activate BMP signaling pathway. Structures of Bmp2a, Bmp2b, Bmp4 and Bmp16 were found to be remarkably similar, with residues involved in receptor binding being highly conserved All proteins could activate the BMP signaling pathway, suggesting that they share a common function. On the contrary, stage-and tissue-specific expression of bmp2, bmp4 and bmp16 suggested the genes might be differentially regulated (e.g. different transcription factors, enhancers and/or regulatory modules) but also that they are involved in distinct physiological processes, although with the same function. Retinoic acid, a morphogen known to interact with BMP signaling during bone formation, was shown to down-regulate the expression of bmp2, bmp4 and bmp16, although to different extents. Taxonomic and phylogenetic analyses indicated that bmp16 diverged before bmp2 and bmp4, is not restricted to teleost fish lineage as previously reported, and that it probably arose from a whole genomic duplication event that occurred early in vertebrate evolution and disappeared in various tetrapod lineages through independent events.

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Section: Discussionmentioning

confidence: 65%

Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives

Marques

Fernández

Viegas

et al. 2015

Cell. Mol. Life Sci.

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“…neurotransmitter), which binds to a brain cell membrane receptor. Osmotic homeostasis in fish is maintained by multiple osmosensors that activate neural and hormonal signals that are involved in regulating ion and water transport across osmoregulatory tissues (Kültz, 2012). These signaling events, which precede the induction of the MIB pathway in tilapia brain cells, are yet to be investigated in fish.…”

Section: Mib Enzymes In Tilapia Brain Robustly Respond To Plasma Osmomentioning

confidence: 99%

Tilapia (Oreochromis mossambicus) brain cells respond to hyperosmotic challenge by inducingmyo-inositol biosynthesis

Gardell

Yang

Sacchi

et al. 2013

Journal of Experimental Biology

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SUMMARYThis study aimed to determine the regulation of the de novo myo-inositol biosynthetic (MIB) pathway in Mozambique tilapia (Oreochromis mossambicus) brain following acute (25 ppt) and chronic (30, 60 and 90 ppt) salinity acclimations. The MIB pathway plays an important role in accumulating the compatible osmolyte, myo-inositol, in cells in response to hyperosmotic challenge and consists of two enzymes, myo-inositol phosphate synthase and inositol monophosphatase. In tilapia brain, MIB enzyme transcriptional regulation was found to robustly increase in a time (acute acclimation) or dose (chronic acclimation) dependent manner. Blood plasma osmolality and Na + and Cl -concentrations were also measured and significantly increased in response to both acute and chronic salinity challenges. Interestingly, highly significant positive correlations were found between MIB enzyme mRNA and blood plasma osmolality in both acute and chronic salinity acclimations. Additionally, a mass spectrometry assay was established and used to quantify total myo-inositol concentration in tilapia brain, which closely mirrored the hyperosmotic MIB pathway induction. Thus, myo-inositol is a major compatible osmolyte that is accumulated in brain cells when exposed to acute and chronic hyperosmotic challenge. These data show that the MIB pathway is highly induced in response to environmental salinity challenge in tilapia brain and that this induction is likely prompted by increases in blood plasma osmolality. Because the MIB pathway uses glucose-6-phosphate as a substrate and large amounts of myo-inositol are being synthesized, our data also illustrate that the MIB pathway likely contributes to the high energetic demand posed by salinity challenge.

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“…Such species have evolved sophisticated mechanisms of hydromineral balance that regulate the major ions in seawater [14] and this regulation is mainly achieved by the cooperative function of the gills, intestine, and kidney [13,[15][16][17]. The gastrointestinal tract (GI tract) of marine fish plays a critical role in osmoregulation via the process of NaCl coupled fluid absorption [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Fortification of an Aquafeed with Potassium Chloride Does Not Improve Survival of Juvenile Australian Snapper Pagrus auratus Reared in Potassium Deficient Saline Groundwater

Booth¹,

Fielder²

2016

Fishes

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This study was done to determine if fortification of a commercial aquafeed with KCl could improve the survival of juvenile Australian snapper Pagrus auratus reared in K + deficient saline groundwater (KDSGW; <5 mg K + L −1 ). Experiment 1 (Exp. 1) tested whether feeding an aquafeed fortified with zero, 25, or 50 g KCl kg −1 for 6 days affected feed intake and survival of fish transferred immediately from estuarine water to KDSGW of the equivalent salinity (20 g·L −1 ). Experiment 2 (Exp. 2) investigated whether an aquafeed fortified with zero, 10, or 25 g KCl kg −1 affected survival, feed intake, and growth rate (SGR) of snapper reared in KDSGW fortified to have 40% or 100% the [K + ] of equivalent salinity estuarine water (20 g·L −1 ). The results of Exp. 1 demonstrated there was no benefit of fortifying aquafeed with KCl; fish transferred into KDSGW stopped feeding and developed symptoms akin to tetany. Some individuals also died and others became moribund. Exp. 1 was terminated according to animal care and ethics guidelines. The results of Exp. 2 indicated the amount of KCl added to the aquafeed did not affect survival, feed intake, or food conversion ratio (FCR) of snapper, irrespective of water treatment. However, SGR and FCR was better when fish were reared in normal estuarine water and KDSGW fortified to have 100% the [K + ] of equivalent salinity estuarine water. Our results demonstrated that juvenile snapper were unable to utilize the KCl added to the aquafeed and were probably reliant on sequestering K + ions from the water column in order to maintain functions involving hydromineral homeostasis. Fortification of aquafeeds with KCl does not ameliorate the negative effects of KDSWG on the survival of juvenile snapper.

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The Combinatorial Nature of Osmosensing in Fishes

Cited by 84 publications

References 189 publications

Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives

Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives

Tilapia (Oreochromis mossambicus) brain cells respond to hyperosmotic challenge by inducingmyo-inositol biosynthesis

Fortification of an Aquafeed with Potassium Chloride Does Not Improve Survival of Juvenile Australian Snapper Pagrus auratus Reared in Potassium Deficient Saline Groundwater

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