The Combined Effect of the Organic Modifier Content and pH of the Mobile Phase on the Chromatographic Behavior of Some Arylpropionic and Arylacetic Acids to Optimize Their Liquid Chromatographic Determinations

Secilmis-Canbay, Hale; Çubuk-Demiralay, Ebru; Alsancak, Güleren; Özkan, Síbel A.

doi:10.1007/s10337-012-2255-z

Cited by 7 publications

(2 citation statements)

References 24 publications

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“…Kromatografide alıkonma tahminine yönelik denklemleri kullanmak daha kısa sürede deneyin gerçekleştirilmesi ve bileşiklerin ayrımını sağlamaktadır [11,15]. Hareketli fazda organik çözücünün hacimce yüzdesiyle kapasite faktörü değerinin ilişkisi Eşitlik 1'de verilmiştir.…”

Section: Abstract: Antifungal Drug Active Ingredients Rplc Methods Op...unclassified

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

BAŞAT DERELİ,

ÇUBUK DEMİRALAY,

KONÇE

2023

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

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Bu çalışmada azol türevi antifungal ilaç etken maddeleri klotrimazol, lanokonazol ve klimbazolün ters faz sıvı kromatografi (RPLC) yöntemi kullanılarak geliştirilen metot optimizasyonunda, bileşiklerin kapasite faktörü değerleri ve analizlendikleri hareketli fazdaki metanol derişimi ilişkisi esas alınmıştır. İyonlaşan gruba sahip bu bileşiklerin optimizasyonunda hareketli faz pH değeride optimizasyonda bir diğer etkendir. İzokratik bir ayırma için kapasite faktörü değerlerinin 1-5 aralığında olması tercih edilmektedir. RPLC yöntemiyle gerçekleştirilen klotrimazol, lanokonazol ve klimbazol bileşiklerinin aynı anda tayini için yapılan optimizasyonda %50, %55 ve %60 (v/v) metanol içeren metanol-su ikili karışımları kullanılmıştır. Ayırma için çalışılan ortamlarda iki hareketli faz pH değerindeki (pH 4,0 ve 5,5) alıkonma zamanları belirlenmiştir. Hidrofobik özellikteki bileşiklerin tayini için Zorbax SB-CN (4,6x150mm) kolon tercih edilmiştir. Kolon sıcaklığı 37oC’de ve akış hızı da 1 mL/dakikada sabitlenmiştir. Optimizasyon koşullarının belirlenmesinde logk- % (v/v) metanol ilişkisi ve bileşiklerin ayrılmalarını gösteren seçicilik faktörü (α) parametresinden yararlanılmıştır.

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Section: Abstract: Antifungal Drug Active Ingredients Rplc Methods Op...unclassified

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

BAŞAT DERELİ,

ÇUBUK DEMİRALAY,

KONÇE

2023

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

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“…There are several approaches to predict the retention behavior of an ionisable solute as a function of chromatographic conditions. Because of the ionization properties of these types of solutes, the factors generally selected to optimize the chromatographic separation are the pH of the mobile phase and the percentage of the organic solvent of the eluent [3][4][5][6][7][8][9]. The effect of these parameters on the chromatographic behavior is somewhat more complex since the variation of organic modifier and analyze these compounds.…”

Section: Introductionmentioning

confidence: 99%

Identification of Penicillinase-Resistant Penicillins in Human Urine by RPLC Method

Üstün¹,

Demıralay²,

Nane³

2016

Eurasian J Anal Chem

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In this study, the combined effect of the organic modifier content and mobile phase pH has been used to predict the chromatographic behavior of methicillin, oxacillin, nafcillin, cloxacilin and dicloxacillin. The optimum separation condition of these drugs in acetonitrilewater binary mixture was determined from the mobile phase pH dependence of their retention factor. A Synergy Fusion-RP C18 analytical column (150 mm x 4.6 mm I.D., 4 µm) was preferred to carry out the developed method. Chromatographic measurements were done at 30• C with an eluent flow rate of 1 mL min -1 . In order to validate the optimized conditions, these drugs were applied successfully in spiked samples of human urine. Valsartan was chosen as an internal standard. The described method was linear over a concentration range of 2-32 µg mL -1 and 3-48 µg mL -1 for the assay of methicillin, nafcillin, dicloxacillin and oxacillin, cloxacillin respectively. Good linear relationships over the investigated concentration ranges were observed with values of correlation coefficient higher than 0.999 for all of the drugs. The intra-day and inter-day precisions of this method were evaluated with RSD (%) values less than 0,561 and 1,622%, respectively. Optimized assay method was validated according to United States Pharmacopeia (USP) guidelines to confirm linearity, accuracy and precision.

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Determination of chromatographic dissociation constants of some carbapenem group antibiotics and quantification of these compounds in human urine

Demıralay

Koç

DALDAL

et al. 2013

Biomedical Chromatography

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The dissociation constant values (s (s) pKa ) of some carbapenem group drugs (ertapenem, meropenem, doripenem) in different percentages of methanol-water binary mixtures (18, 20 and 22%, v/v) were determined from the mobile phase pH dependence of their retention factor. Evaluation of these data was performed using the NLREG program. From calculated pKa values, the aqueous pKa values of these subtances were calculated by different approaches. Moreover, the correlation established between retention factor and the pH of the water-methanol mobile phase was used to determine the optimum separation conditions. In order to validate the optimized conditions, these drugs were studied in human urine. The chromatographic separation was realized using a Gemini NX C18 column (250 × 4.6 mm i.d., 5 µm particles) and UV detector set at 220 and 295 nm.

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The Combined Effect of the Organic Modifier Content and pH of the Mobile Phase on the Chromatographic Behavior of Some Arylpropionic and Arylacetic Acids to Optimize Their Liquid Chromatographic Determinations

Cited by 7 publications

References 24 publications

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

Identification of Penicillinase-Resistant Penicillins in Human Urine by RPLC Method

Determination of chromatographic dissociation constants of some carbapenem group antibiotics and quantification of these compounds in human urine

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